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Osteocyte cell lines

  • US 6,358,737 B1
  • Filed: 09/11/1998
  • Issued: 03/19/2002
  • Est. Priority Date: 07/31/1996
  • Status: Expired due to Term
First Claim
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1. A method for the production of an osteocyte cell line, said cell line having the following properties:

  • available to passage more than 20 times, a stellate shape with dendritic processes and expresses increased levels of osteocalcin compared to mouse osteoblast or osteoblast-like cell lines, and said method comprising the steps of;

    (a) isolating bones from a transgenic mouse whose genome contains an osteocalcin promoter driven T-antigen transgene and preparing bone pieces from the bones;

    (b) digesting the bone pieces with collagenase solution and harvesting cells into fetal and adult calf serum supplemented medium; and

    (c) plating the harvested cells and isolating said cell line by selecting a single colony.

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