Antisense inhibition of ras gene with chimeric and alternating oligonucleotides
First Claim
1. An oligonucleotide comprising from 8 to 30 nucleotide units specifically hybridizable with selected DNA or mRNA deriving from the human H-ras gene and containing a first region having at least one nucleosidic unit modified at the 2′
- position with a 2′
-O-methoxyethyl substituent and a second region having at least one phosphorothioate linkage.
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Accused Products
Abstract
Compositions and methods are provided for the modulation of expression of the human ras gene in both the normal and activated forms. Oligonucleotides are provided that have methylene(methylimino) linkages alternating with phosphorothioate or phosphodiester linkages. Further oligonucleotides are provide that have a first region having a methylene(methylimino) linkage alternating with a phosphorothioate or phosphodiester linkage and a second region having phosphorothioate linkages. Such oligonucleotides can be used for diagnostics as well as for research purposes including methods for diagnosis, detection and treatment of conditions arising from the activation of the H-ras gene.
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Citations
8 Claims
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1. An oligonucleotide comprising from 8 to 30 nucleotide units specifically hybridizable with selected DNA or mRNA deriving from the human H-ras gene and containing a first region having at least one nucleosidic unit modified at the 2′
- position with a 2′
-O-methoxyethyl substituent and a second region having at least one phosphorothioate linkage. - View Dependent Claims (2, 3, 4, 5, 6)
- position with a 2′
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7. An oligonucleotide comprising from 8 to 30 nucleotide units hybridizable with selected DNA or mRNA deriving from the human H-ras gene and containing at least one nucleosidic unit modified at the 2′
- position with a 2′
-O-methoxyethyl substituent. - View Dependent Claims (8)
- position with a 2′
Specification