Method and apparatus for determining chemical properties

• US 6,360,585 B1
• Filed: 03/06/2000
• Issued: 03/26/2002
• Est. Priority Date: 03/06/2000
• Status: Expired due to Fees

• Current Assignee: General Electric Company
• Original Assignee: General Electric Company
• Inventors: Potyrailo, Radislav Alexandrovich, Sivavec, Timothy Mark
• Primary Examiner(s): KWOK, HELEN C
• Application Number:
  US09/519,895
• Time in Patent Office: 750 Days
• Field of Search: 735/79, 735/97, 735/90, 73/240.1, 73/240.6, 310/340, 310/367, 310/369, 310/365, 310/311, 310/321, 422/83, 422/98
• US Class Current: 73/24.06
• CPC Class Codes: G01N 2291/0256 Adsorption, desorption, surface mass change, e.g. on biosensors
• CPC Class Codes: G01N 29/036 by measuring frequency or resonance of acoustic waves

Abstract

A method and apparatus for determining chemical properties. The method is based on the sequential measurement of the variation of the oscillation frequency of a single sensing device when different chemically sensitive film materials are deposited on both sides of a resonator such as a quartz crystal microbalance (QCM). Each of the sides of the resonator is sequentially exposed to an analyte while another side is exposed to a blank gas. In this way, the analyte-dependent signal from the resonator is generated only from a single film. Measurements are further made by switching the analyte stream to expose the sensing film on another side of the resonator previously exposed to a blank.

Claims

1. An apparatus for determining chemical properties of at least one chemical species in a sample of an analyte, the apparatus comprising:

a vessel divided into a plurality of compartments;

at least one resonator comprising a first side coated with a first sensing film comprising a first material and a second side coated with a second sensing film comprising a second material different than said first material, each of said sides of said at least one resonator being exposed to a different one of said plurality of compartments, each of said first and second materials having a respective differentiable affinity toward said at least one chemical species and being capable of inducing a respective differentiable resonant frequency in said at least one resonator upon contact with said at least one chemical species, one of said materials contacting said sample of said analyte when another of said materials contacting a reference chemical, and each of said materials contacting said sample of said analyte at a different time;

an electric power source coupled to said at least one resonator and adapted to place an oscillating electric potential between said first side and said second side of said at least one resonator; and

a frequency detector coupled to said at least one resonator and adapted to detect the frequency of resonation of said at least one resonator;

wherein said at least one resonator generates differing resonant frequencies when each of said materials contacts said sample of said analyte, respectively; and

said differing resonant frequencies provide a determination of said chemical properties of said at least one chemical species.

2. An apparatus according to claim 1, wherein said vessel comprises at least first and second compartments and wherein said first side of said at least one resonator is exposed to said first compartment and said second side of said at least one resonator is exposed to said second compartment.

3. An apparatus according to claim 2, wherein said first sensing film and said second sensing film comprise different chemical compositions.

4. An apparatus according to claim 3, wherein said at least one resonator comprises at least one quartz crystal microbalance resonator.

5. An apparatus according to claim 4, the apparatus further comprising a first wall disposed in said vessel to divide said vessel into a first compartment and a second compartment, said at least one quartz crystal microbalance resonator comprising at least a portion of said first wall.

6. An apparatus according to claim 4, wherein said at least one quartz crystal microbalance resonator comprises an AT-cut quartz crystal substrate that comprises a first side coated with PIB and a second side coated with an amorphous fluoropolymer.

7. An apparatus according to claim 4, wherein said at least one quartz crystal microbalance resonator comprises an AT-cut quartz crystal substrate that comprises a first side coated with a hard-soft block elastomer and a second side coated with PECH.

8. An apparatus according to claim 3, further comprising a second resonator that comprises a first side coated with a third sensing film and a second side coated with a fourth sensing film.

9. An apparatus according to claim 8, wherein said vessel is divided into first, second, and third compartments and said first side of said second resonator is exposed to said second compartment and said second side of said second resonator is exposed to said third compartment.

16. An apparatus according to claim 4, wherein said vessel is divided into at least first and second compartments and wherein a first side of said at least one quartz crystal microbalance resonator is exposed to a first compartment of said vessel, and a second side of said at least one quartz crystal microbalance resonator is exposed to a second compartment of said vessel.

19. An apparatus according to claims 4, further comprising a second quartz crystal microbalance resonator having a first side coated with a third sensing film and a second side coated with a fourth sensing film.

20. An apparatus according to claim 19, wherein said vessel is divided into first, second, and third compartments and said first side of said second quartz crystal microbalance resonator is exposed to said second compartment, and said second side of said second quartz crystal microbalance resonator is exposed to said third compartment.

10. A method for determining chemical composition, the method comprising the steps of:

a first step of exposing a first side of at least one resonator coated with a first sensing film to an analyte and exposing a second side of the at least one resonator coated with a second sensing film to a reference chemical, said first and second sensing films comprising different materials that have respective differentiable affinities toward chemical species of said chemical composition and that are capable of inducing respective differentiable resonant frequencies in said at least one resonator;

a first step of measuring a fundamental frequency of the at least one resonator during the first step of exposing;

a second step of exposing a second side of the at least one resonator to the analyte and exposing the first side of the at least one resonator to the reference chemical;

a second step of measuring the fundamental frequency of the at least one resonator during the second step of exposing; and

determining the chemical composition of the analyte based on the first step of measuring and the second step of measuring.

11. A method according to claim 10, wherein said step of determining comprises determining analyte type by comparing results of said first step of measuring and said second step of measuring with normalized resonator responses.

12. A method according to claim 10, wherein said step of determining comprises determining analyte concentration by using multivariate analysis techniques.

13. An apparatus for determining chemical composition, the apparatus comprising:

first means for measuring a fundamental frequency of at least one resonator, a first side of which is coated with a first sensing film and a second side of which is coated with a second sensing film while the first side of the at least one resonator is exposed to an analyte and the second side is exposed to a reference chemical, said first and second sensing films comprising different materials that have respective differentiable affinities toward chemical species of said chemical composition and that are capable of inducing respective differentiable resonant frequencies in said at least one resonator;

second means for measuring the fundamental frequency of the at least one resonator while the second side of the at least one resonator is exposed to the analyte and the first side of the at least one resonator is exposed to the reference chemical; and

means for determining chemical composition of the analyte based on results obtained by said first means for measuring and said second means for measuring.

14. An apparatus according to claim 13, wherein said means for determining comprises means for determining analyte type by comparing the results obtained by said first means for measuring and said second means for measuring with normalized resonator responses.

15. An apparatus according to claim 13, wherein said means for determining comprises means for determining analyte concentration by using multivariate analysis techniques.

17. An apparatus according to claim 15, wherein said at least one resonator comprises an AT-cut quartz crystal substrate that comprises a first side coated with PIB and a second side coated with an amorphous fluoropolymer.

18. An apparatus according to claim 15, wherein said at least one resonator comprises an AT cut quartz crystal substrate that comprises a first side coated with a hard-soft block elastomer and a second side coated with PECH.