Coaxial-drive centrifuge providing tilt control relative to centrifugal force

• US 6,361,486 B1
• Filed: 06/08/2000
• Issued: 03/26/2002
• Est. Priority Date: 02/29/2000
• Status: Expired due to Fees

• Current Assignee: Agilent Technologies Europe BV (Agilent Technologies, Inc.)
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Gordon, Gary B.
• Primary Examiner(s): Cooley, Charles E.
• Application Number:
  US09/590,934
• Time in Patent Office: 656 Days
• Field of Search: 494/1, 494/7-9, 494/10, 494/19, 494/31, 494/33, 494/84, 366/217, 422/72
• US Class Current: 494/19
• CPC Class Codes: B01J 2219/0049 by centrifugation
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00707 separated from the reactor apparatus
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B04B 5/02 Centrifuges consisting of a plurality of separate bowls rotating round an axis situated between the bowls
• CPC Class Codes: B04B 9/10 Control of the drive; Speed regulating for solid bowl screw centrifuges B04B1/2016
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries

Abstract

A centrifuge includes a centrifuge rotor that rotates to provide supergravity conditions and tilt rotors that provide for sample agitation under the supergravity conditions. The centrifuge rotor is integral with a hollow centrifuge drive shaft. A tilt-drive shaft extends through the hollow and rotates coaxially of the centrifuge rotor. The tilt-drive shaft has a pinion that engages the tilt rotors, which thus are made to rotate about tilt axes that are spaced from and parallel to the centrifuge axis. A centrifuge motor is mechanically coupled to the centrifuge drive shaft to rotate the centrifuge rotor. A tilt motor is mechanically coupled to the tilt drive shaft for imparting a rocking motion of the tilt rotors (and thus of mounted sample reaction cells) relative to the centrifuge rotor. Both motors are controlled by a servo that receives orientation information from orientation encoders associated with the motors; the tilt motor is phase locked to the centrifuge motor to ensure precise relative motion control. The centrifuge motor is typically rotated to achieve a centrifugal force at the tilt rotors of about 1000 G. The tilt motor is controlled differentially relative to the centrifuge motor so that the tilt rotors rotate controllably, at, above and/or below the centrifuge rotation rate to achieve the desired rotation relative to the centrifuge. This centrifuge provides a robust and precise method of controlling fluid motion within a sample container.

Claims

1. A centrifuge for tilting a sample container under supergravity conditions, said centrifuge comprising:

a centrifuge rotor, said centrifuge rotor being rotatable about a centrifuge axis;

a tilt rotor, said tilt rotor being rotatably coupled to said centrifuge rotor so as to be rotatable about a tilt axis not coincident with said centrifuge axis;

centrifuge drive means for rotating said centrifuge rotor; and

tilt-drive means including a tilt motor for rotating said tilt rotor, said tilt-drive means including a tilt-drive shaft extending along said centrifuge axis and being rotatable about said centrifuge axis; and

servo-control means for controlling the orientation of said tilt rotor by controlling said tilt motor in part as a function of the orientation of said centrifuge rotor.

2. A centrifuge as recited in claim 1 wherein said centrifuge drive means includes a centrifuge motor that controls the orientation of said centrifuge rotor, said tilt-drive means controlling the orientation of said tilt rotor via said tilt-drive shaft.

3. A centrifuge as recited in claim 2 wherein said centrifuge motor and said tilt motor are stationary.

4. A centrifuge as recited in claim 1 further comprising centrifuge orientation encoder means for providing indications of the orientation of said centrifuge rotor, said centrifuge orientation encoder means being coupled to said servo-control means for providing said indications thereto.

5. A centrifuge as recited in claim 1 further comprising encoder means for indicating the orientations of said tilt rotor and said centrifuge rotor to said servo-control means.

6. A centrifuge as recited in claim 1 wherein said tilt rotor includes means for coupling said sample container rigidly thereto.

7. A centrifuge as recited in claim 1 wherein said tilt axis is more parallel to said centrifuge axis than orthogonal to it.