Beta-1,3-galactosyltransferase homolog, ZNSSP6

• US 6,361,985 B1
• Filed: 01/12/2000
• Issued: 03/26/2002
• Est. Priority Date: 01/12/1999
• Status: Expired due to Fees

• Current Assignee: Zumogenetics, Inc.
• Original Assignee: Zymogenetics Incorporated (Bristol-Myers Squibb Company)
• Inventors: Yamamoto, Gayle, Jaspers, Stephen R., Gao, Zeren, Conklin, Darrell C.
• Primary Examiner(s): Achutamurthy, Ponnathapura
• Assistant Examiner(s): BRAGDON, KATHLEEN KERR
• Application Number:
  US09/482,180
• Time in Patent Office: 804 Days
• Field of Search: 536/23.1, 536/23.2, 435/320.1, 435/183, 435/252.3, 435/254.11, 435/419, 435/325, 435/193
• US Class Current: 435/193
• CPC Class Codes: C12N 9/1051 Hexosyltransferases (2.4.1)

Abstract

The present invention relates to polynucleotide and polypeptide molecules for znssp6, a novel member of the galactosyltransferase family. The polypeptides, and polynucleotides encoding them, are cell-cell interaction and glycoprotein synthesis modulating and may be used for delivery and therapeutics. The present invention also includes antibodies to the znssp6 polypeptides.

Claims

1. An isolated polynucleotide encoding a polypeptide wherein the polypeptide comprises residues 114 to 370 of SEQ ID NO:

2.

2. The isolated polynucleotide according to claim 1, wherein the polypeptide molecule comprises residues 114 to 378 of SEQ ID NO:

2.

3. The isolated polynucleotide according to claim 2, wherein the polypeptide molecule comprises residues 50 to 378 of SEQ ID NO:

2.

4. The isolated polynucleotide according to claim 3, wherein the polypeptide molecule comprises residues 26 to 378 of SEQ ID NO:

2.

5. The isolated polynucleotide according to claim 2, wherein the polypeptide molecule comprises residues 1 to 378 of SEQ ID NO:

2.

6. The isolated polynucleotide according to claim 1, wherein the polynucleotide comprises a nucleotide sequence from nucleotide 474 to nucleotide 1244 of SEQ ID NO:

1.

7. The isolated polynucleotide according to claim 6, wherein said polynucleotide further comprises nucleotide 474 to nucleotide 1268 of SEQ ID NO:

1.

8. The isolated polynucleotide according to claim 6, wherein said polynucleotide further comprises nucleotide 135 to nucleotide 1244 of SEQ ID NO:

1.

9. The isolated polynucleotide according to claim 6, wherein said polynucleotide further comprises nucleotide 135 to nucleotide 1268 of SEQ ID NO:

1.

10. An expression vector comprising the following operably linked elements:

a transcription promoter;

a DNA segment wherein the DNA segment is a polynucleotide encoding the polypeptide of claim 1; and

a transcription terminator.

11. The expression vector according to claim 10 wherein the DNA segment further encodes an affinity tag.

12. A cultured cell into which has been introduced an expression vector according to claim 10, wherein said cell expresses the polypeptide encoded by the DNA segment.

13. A method of producing a polypeptide comprising culturing a cell according to claim 12, whereby said cell expresses the polypeptide encoded by the DNA segment;

and recovering the polypeptide.