Method and system for overlaying at least three microarray images to obtain a multicolor composite image

• US 6,362,832 B1
• Filed: 09/01/1999
• Issued: 03/26/2002
• Est. Priority Date: 09/01/1999
• Status: Expired due to Term

• Current Assignee: Packard Bioscience Co. (Massachusetts) (Revvity, Inc.)
• Original Assignee: Packard Bioscience Co. (Massachusetts) (Revvity, Inc.)
• Inventors: Yang, Jun, Stephan, Todd J., Noblett, David A.
• Primary Examiner(s): Luu, Matthew
• Assistant Examiner(s): GOOD JOHNSON, MOTILEWA
• Application Number:
  US09/388,204
• Time in Patent Office: 937 Days
• Field of Search: 345/435, 345/629, 345/632, 382/129, 382/284
• US Class Current: 345/629
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G02B 21/004 fixed arrays, e.g. switchable aperture arrays
• CPC Class Codes: G02B 21/0076 arrangements using fluorescence or luminescence
• CPC Class Codes: G02B 21/008 Details of detection or image processing, including general computer control

Abstract

Method and system for overlaying at least three microarray images to obtain a multicolor composite image, which is then displayed on a monitor of a computer system. The microarray images are taken from a microarray scanner of a DNA microarray and can be viewed simultaneously through the use of the image overlays where each image is represented by a different color. Each pixel of the composite image is generated by the OR operator applied to all corresponding pixels of the microarray images. Registration of the microarray images can be altered with a keyboard or mouse of the computer system.

Claims

1. A method for overlaying at least three microarray images to obtain a multicolor composite image, the method comprising:

providing at least three microarray images wherein each of the images includes a plurality of pixels having brightness values;

assigning each image a different color to obtain colorized images;

combining corresponding pixels of each colorized image to obtain overlayed images whereby the overlayed images form a multicolor composite image; and

displaying the multicolored composite image.

2. The method as claimed in claim 1 wherein the overlayed images have a registration and wherein the method further comprises processing the overlayed images to alter the registration of the overlayed images.

3. The method as claimed in claim 2 wherein the step of processing includes the step of receiving a command to move one of the overlayed images relative to the other overlayed images.

4. The method as claimed in claim 1 wherein the step of providing includes the step of scanning a microarray at at least three different wavelengths to obtain the microarray images.

5. The method as claimed in claim 4 wherein the step of assigning includes the step of creating a palette having entries for each of the wavelengths to display the microarray images, the entries for each palette being based on a composite color and the brightness values of the pixels of its respective image.

6. The method as claimed in claim 1 wherein the step of combining includes the step of logically ORing corresponding pixels of each colorized image.

7. The method as claimed in claim 1 further comprising receiving at least one command to select a composite color for each microarray image to be overlayed.

8. A system for overlaying at least three microarray images to obtain a multicolor composite image, the system comprising:

means for providing at least three microarray images wherein each of the images includes a plurality of pixels having brightness values;

a computer programmed to;

assign each image a different color to obtain colorized images; and

combine corresponding pixels of each colorized image to obtain overlayed images whereby the overlayed images form a multicolor composite image; and

a display for displaying the multicolor composite image.

9. The system as claimed in claim 8 wherein the overlayed images have a registration and wherein the computer is further programmed to process the overlayed images to alter the registration of the overlayed images.

10. The system as claimed in claim 9 wherein the computer is programmed to receive a command to move one of the overlayed images relative to the other overlayed images.

11. The system as claimed in claim 8 wherein the means for providing includes a microarray scanner for scanning a microarray at at least three different wavelengths to obtain the microarray images.

12. The system as claimed in claim 11 wherein the computer is programmed to create a palette having entries for each of the wavelengths to display the microarray images, the entries for each palette being based on a composite color and the brightness values of the pixels of its respective image.

13. The system as claimed in claim 8 further comprising a memory buffer and wherein the computer is further programmed to assign each pixel in one of the colorized images to the memory buffer and logically ORing corresponding pixels in the remaining colorized images with the pixels in the memory buffer to obtain the overlayed images.

14. The system as claimed in claim 8 wherein the computer is further programmed to receive at least one command to select a composite color for each microarray image to be overlayed.