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Arrays of protein-capture agents and methods of use thereof

  • US 6,365,418 B1
  • Filed: 05/18/2000
  • Issued: 04/02/2002
  • Est. Priority Date: 07/14/1998
  • Status: Expired due to Term
First Claim
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1. An array device comprisinga substrate having a surface;

  • and, one or more protein immobilization regions on said surface, said protein immobilization regions each comprising;

    (i) an ordered hydrophobic monolayer formed of alkyl chains having proximal ends which are chemisorbed or physisorbed to said surface within said immobilization regions, and opposite hydrophobic distal ends, (ii) a hydrophilic monolayer attached to said ordered hydrophobic monolayer, said hydrophilic monolayer comprising hydrophilic chains, each hydrophilic chain having a proximal end by which said hydrophilic chain is linked to an alkyl chain distal end, and an opposite hydrophilic distal end, and, (iii) a plurality of functional groups attached to said hydrophilic/hydrophobic monolayers for covalently attaching protein-capture agents to said hydrophilic/hydrophobic monolayers within said immobilization regions;

    wherein said hydrophobic monolayer and hydrophilic monolayers together are effective to resist non-specific protein binding.

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