Minimally invasive methods for measuring analtes in vivo
First Claim
1. A method for detecting an analyte contained in the interstitial fluid of a body comprising the steps of:
- (a) providing at least one sensor particle comprising a substance with a receptor and a signal fluor, wherein the receptor preferentially recognizes the analyte, and the binding of the receptor to the analyte makes the substance to undergo a photoinduced electron transfer (PET) whereby a detectable analyte signal is generated in response to the analyte concentration of the body, wherein the sensor particle is selected from the group consisting of;
a hydrophilic particle with the receptor bound to the inside of the particle, hydrophobic particle with the receptor captured inside the particle, and hydrophobic insoluble particle with the receptor coupled to the surface of the particle;
(b) placing the sensor particle into the skin of the body for allowing the sensor particle to be in contact with the interstitial fluid of the body to undergo a PET and to generate the detectable analyte signal;
(c) detecting the generated analyte signal; and
(d) determining the concentration of the analyte contained in the interstitial fluid.
1 Assignment
0 Petitions
Accused Products
Abstract
Minimally invasive methods for measuring an analyte, such as glucose, contained in tile interstitial fluid of a body are provided. The methods include the steps of.
(a) providing at least one sensor particle capable of generating a detectable analyte signal in responding to the analyte concentration of the body,
(b) placing the sensor particle into the skin of the body for allowing the sensor particle to be in contact with the interstitial fluid of the body to generate the detectable analyte signal,
(c) detecting the generated analyte signal, and
(d) determining the concentration of the analyte contained in the interstitial fluid.
The sensor particles may be made to be responsive to an analyte such as glucose concentration contained in a body fluid by including a photo-induced electron transfer receptor specific for the analyte in the sensor particle.
-
Citations
31 Claims
-
1. A method for detecting an analyte contained in the interstitial fluid of a body comprising the steps of:
-
(a) providing at least one sensor particle comprising a substance with a receptor and a signal fluor, wherein the receptor preferentially recognizes the analyte, and the binding of the receptor to the analyte makes the substance to undergo a photoinduced electron transfer (PET) whereby a detectable analyte signal is generated in response to the analyte concentration of the body, wherein the sensor particle is selected from the group consisting of;
a hydrophilic particle with the receptor bound to the inside of the particle, hydrophobic particle with the receptor captured inside the particle, and hydrophobic insoluble particle with the receptor coupled to the surface of the particle;
(b) placing the sensor particle into the skin of the body for allowing the sensor particle to be in contact with the interstitial fluid of the body to undergo a PET and to generate the detectable analyte signal;
(c) detecting the generated analyte signal; and
(d) determining the concentration of the analyte contained in the interstitial fluid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 24)
-
-
13. A method for detecting an analyte contained in the interstitial fluid of a body comprising the steps of:
-
(a) providing at least one sensor particle comprising a substance capable of undergoing a photoinduced electron transfer (PET) and generating a detectable analyte signal in response to the analyte concentration of the body;
(b) placing the sensor particle into the skin of the body by tattooing technique, whereby the sensor particle is contacted with the interstitial fluid of the body to undergo a PET and to generate the detectable analyte signal;
(c) detecting the generated analyte signal; and
(d) determining the concentration of the analyte contained in the interstitial fluid.
-
-
17. A method for detecting an analyte contained in the interstitial fluid of a body comprising the steps of:
-
(a) providing at least one sensor particle comprising at least two different types of receptors with signal fluors, wherein the receptors preferentially recognize the analyte;
(b) placing the sensor particle into the skin of the body for allowing the sensor particle to be in contact with the interstitial fluid of the body to bind analyte and undergo a photoinduced electron transfer (PET) whereby a detectable analyte signal generated in response to the analyte concentration in the body;
(c) detecting the generated analyte signal; and
(d) determining the concentration of the analyte contained in the interstitial fluid. - View Dependent Claims (18, 19, 20, 21)
-
-
22. A method for detecting an analyte contained in the interstitial fluid of a body comprising the steps of:
-
(a) providing at least one sensor particle comprising a first substance capable of generating an analyte signal that is responsive to the analyte concentration of the body and a second substance capable of generating a reference signal that is independent of the analyte concentration of the body, wherein the first substance is a collection of different receptors with signal fluors and the receptors preferentially recognize the analyte;
(b) placing the sensor particle into the skin of the body for allowing the sensor particle to be in contact with the interstitial fluid of the body to generate the detectable analyte signal;
(c) detecting the generated analyte signal; and
(d) determining the concentration of the analyte contained in the interstitial fluid. - View Dependent Claims (23, 25, 26, 27)
-
-
28. A method for detecting an analyte contained in the interstitial fluid of a body comprising the steps of:
-
(a) providing at least one sensor particle comprising a substance capable of undergoing a photoinduced electron transfer (PET) and generating a detectable analyte signal in response to the analyte concentration of the body, wherein the sensor particle is made of a bio-resorbable polymer, wherein the bio-resorbable polymer is selected from a group consisting of polyglycoic acid (PGA), poly-DL-lactide-co-glycolide (PLGA), starch, gelatin, and the like;
(b) placing the sensor particle into the skin of the body for allowing the sensor particle to be in contact with the interstitial fluid of the body to undergo a PET and to generate the detectable analyte signal;
(c) detecting the generated analyte signal; and
(d) determining the concentration of the analyte contained in the interstitial fluid.
-
-
29. A method for detecting an analyte contained in the interstitial fluid of a body comprising the steps of:
-
(a) providing at least one sensor particle comprising a receptor with a signal fluor, wherein the receptor preferentially recognizes the analyte, and the receptor that is bound to the analyte is a bound receptor and the receptor that is not bound to the analyte is a free receptor;
(b) placing the sensor particle into the skin of the body for allowing the sensor particle to be in contact with the interstitial fluid of the body, whereby the signal fluor contained in the bound receptor generates an analyte signal that is responsive to the concentration of the analyte contained in the interstitial fluid, the signal fluor contained in the free receptor generates a reference signal that is distinguishable from the analyte signal;
(c) detecting the generated analyte signal and the reference signal; and
(d) determining the concentration of the analyte contained in the interstitial fluid. - View Dependent Claims (30, 31)
-
Specification