Non-planar microstructures for manipulation of fluid samples

• US 6,368,871 B1
• Filed: 08/13/1997
• Issued: 04/09/2002
• Est. Priority Date: 08/13/1997
• Status: Expired due to Term

• Current Assignee: Cepheid (Danaher Corporation)
• Original Assignee: Cepheid (Danaher Corporation)
• Inventors: Christel, Lee Allan, Kovacs, Gregory T. A., Petersen, Kurt E., Northrup, M. Allen, McMillan, William A., Pourahmadi, Farzad
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Chorbaji, Monzer R
• Application Number:
  US08/910,434
• Time in Patent Office: 1,700 Days
• Field of Search: 210/767, 204/450, 204/451, 204/452, 204/453, 204/454, 204/455, 204/456, 204/600, 204/601, 204/602, 204/603, 204/604, 204/605, 366/336, 366/340, 366/341, 422/68.1, 422/100, 422/101, 435/287.2, 435/288.5
• US Class Current: 436/180
• CPC Class Codes: B01F 2025/9171 Parallel flow, i.e. every point of the flow moves in parallel layers where intermixing can occur by diffusion or which do not intermix; Focusing, i.e. compressing parallel layers without intermixing them
• CPC Class Codes: B01F 25/31 in conduits or tubes through which the main component flows
• CPC Class Codes: B01F 25/43231 the channels or tubes crossing each other several times
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01L 2200/0636 Focussing flows, e.g. to laminate flows
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: C07H 1/00 Processes for the preparation of sugar derivatives
• CPC Class Codes: G01N 1/405 by adsorption or absorption
• CPC Class Codes: G01N 30/6095 Micromachined or nanomachined, e.g. micro- or nanosize
• CPC Class Codes: Y10S 366/03 Micromixers: variable geometry from the pathway influences mixing/agitation of non-laminar fluid flow
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

This invention comprises an apparatus and method for the manipulation of materials, including particles, cells, macromolecules, such as proteins, nucleic acids and other moieties, in fluid samples. The apparatus comprises an enclosed chamber on a chip having an internal microstructure with surface area substantially greater than the facial surface area of the internal structure. Generally the internal microstructure comprises a continuous network of channels, each of which has a depth substantially greater than its width. The network may comprise a single channel, a single channel with multiple branches, multiple channels, multiple channels with multiple branches, and any combination thereof. The internal structure may present an inert, non-reactive surface, or be coated with a reactive ligand, or be electrically conductive and optionally be coated with an electrical insulator. Discrete portions of the internal structure may differ in structural and surface properties. Multiple chips may be linked together to create a multiplexed array of chambers, optionally linked to other analytical devices.

Claims

1. A device for the manipulation of fluids, the device having at least first and second channels for carrying first and second fluid streams, respectively, wherein the first and second channels converge into a common channel to provide a contact region for the fluid streams, and wherein at least the common channel has a depth greater than its width;

whereby the fluids flowing in the first and second channels flow into the common channel to form at least two fluid streams flowing side by side in the common channel.

2. The device of claim 1, further comprising pillars disposed in the common channel to increase the stability of the fluid streams.

3. The device of claim 1, wherein the first and second channels merge into a plurality of common channels to provide a corresponding plurality of contact regions for the fluid streams, and wherein each of the common channels has a depth greater than its width.

4. The device of claim 3, wherein the device includes at least 50 contact regions/mm².

5. The device of claim 1, wherein the common channel makes at least one 180 degree turn.

6. The device of claim 1, wherein the common channel has a depth greater than 100 um.

7. The device of claim 1, wherein each of the channels has a depth greater than its width.

8. The device of claim 1, wherein each of the channels has a ratio of internal surface area to facial surface area of at least 5 to 1.

9. The device of claim 1, wherein each of the channels has a ratio of internal surface area to facial surface area of at least 10 to 1.

10. The device of claim 1, wherein each of the channels has a ratio of internal surface area to facial surface area of at least 20 to 1.

11. The device of claim 1, wherein the device includes at least three channels converging into the common channel.

12. The device of claim 1, wherein each of the channels has a depth greater than its width.

13. The device of claim 1, wherein the device includes at least six channels converging into the common channel.

14. The device of claim 1, wherein each of the channels has a depth greater than its width.

15. The device of claim 1, further comprising third and fourth channels diverging from the common channel for carrying the first and second fluid streams, respectively, away from the contact region.

16. The device of claim 1, wherein the surfaces of the common channel are hydrophobic.

17. A method for transferring particles between at least two fluids, the method comprising the steps of:

a) providing a device having at least first and second channels for carrying first and second fluid streams, respectively, wherein the first and second channels converge into a common channel to provide a contact region for the fluid streams, and wherein at least the common channel has a depth greater than its width; and

b) forcing first and second fluids to flow through the first and second channels, respectively, and into the common channel so that the two fluid streams flow side by side in the common channel.

18. The method of claim 17, further comprising the step of forcing the first and second fluids to flow through a plurality of common channels to form at least two fluid streams flowing side by side in each of the common channels, wherein each of the common channels has a depth greater than its width.

19. The method of claim 17, wherein each of the channels has a depth greater than its width.

20. The method of claim 17, wherein the common channel has a depth greater than 100 □

m.

21. The method of claim 17, wherein the device further comprises third and fourth channels diverging from the common channel for carrying the first and second fluid streams, respectively, away from the contact region, and wherein the method further comprises the step of forcing the first and second fluid streams to flow into the third and fourth channels, respectively.

22. The method of claim 21, wherein each of the channels has a depth greater than its width.