Internally referenced competitive assays
First Claim
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1. A method for the detection of at least one analyte, which comprises:
- a) applying a sample solution suspected of containing an analyte to a particle zone of a device that can detect such an analyte, the device comprising a solid support containing (1) a particle zone that contains particles containing a first member of an inverse ligand-receptor pair, a first member of a direct ligand-receptor pair, and a signal reagent; and
(2) a first and a second signal zone wherein the first signal zone contains a second member of the inverse ligand-receptor pair and the second signal zone contains a second member of the direct ligand-receptor pair wherein;
the analyte is a member of the inverse ligand-receptor pair or an analog thereof and is not a member of the direct ligand-receptor pair or analog thereof;
b) flowing the solution sequentially through the particle zone and then the signal zones; and
c) determining the ratio of signals between the first and second signal zones.
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Abstract
The present invention provides internally calibrated competitive assays for use on a solid support. Additionally, the invention provides a method of using such assays.
120 Citations
5 Claims
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1. A method for the detection of at least one analyte, which comprises:
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a) applying a sample solution suspected of containing an analyte to a particle zone of a device that can detect such an analyte, the device comprising a solid support containing (1) a particle zone that contains particles containing a first member of an inverse ligand-receptor pair, a first member of a direct ligand-receptor pair, and a signal reagent; and
(2) a first and a second signal zone wherein the first signal zone contains a second member of the inverse ligand-receptor pair and the second signal zone contains a second member of the direct ligand-receptor pair wherein;
the analyte is a member of the inverse ligand-receptor pair or an analog thereof and is not a member of the direct ligand-receptor pair or analog thereof;
b) flowing the solution sequentially through the particle zone and then the signal zones; and
c) determining the ratio of signals between the first and second signal zones. - View Dependent Claims (2, 3, 4, 5)
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Specification
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Current AssigneeMark S. Geisberg
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Original AssigneeMark S. Geisberg
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InventorsGeisberg, Mark S.
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Primary Examiner(s)Chin, Christopher L.
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Assistant Examiner(s)Do, Pensee T.
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Application NumberUS09/578,139Time in Patent Office685 DaysField of Search436/518, 436/514, 436/517, 436/523, 436/526, 436/531, 436/533, 436/544, 436/545, 436/546, 422/55, 422/57, 422/68.1, 422/69, 422/70, 422/82.05, 435/4, 435/7.1, 435/7.5, 435/14, 435/7.94, 435/7.92, 435/7.93, 435/25, 435/28, 435/281.2, 435/288.7, 435/287.9US Class Current436/518CPC Class CodesG01N 33/54306 Solid-phase reaction mechan...G01N 33/54388 based on lateral flowG01N 33/558 using diffusion or migratio...
