Methods of making conditioned cell culture medium compositions
DC CAFCFirst Claim
1. A method of making a composition comprising:
- (a) culturing fibroblast cells in three-dimensions in a cell culture medium sufficient to meet the nutritional needs required to grow the cells in vitro until the cell culture medium contains a desired level of extracellular products so that a conditioned medium is formed;
(b) removing the conditioned medium from the cultured cells; and
(c) combining the conditioned medium with a pharmaceutically acceptable carrier to form the composition.
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Abstract
Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. The medium may be conditioned by stromal cells, parenchymal cells, mesenchymal stem cells, liver reserve cells, neural stem cells, pancreatic stem cells and/or embryonic stem cells. Additionally, the cells may be genetically modified. A three-dimensional tissue construct is preferred. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, formulated with a salve or ointment for topical applications, or, for example, made into or added to surgical glue to accelerate healing of sutures following invasive procedures. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.
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Citations
11 Claims
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1. A method of making a composition comprising:
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(a) culturing fibroblast cells in three-dimensions in a cell culture medium sufficient to meet the nutritional needs required to grow the cells in vitro until the cell culture medium contains a desired level of extracellular products so that a conditioned medium is formed;
(b) removing the conditioned medium from the cultured cells; and
(c) combining the conditioned medium with a pharmaceutically acceptable carrier to form the composition. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method of making a composition comprising:
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(a) culturing fibroblast cells in three-dimensions on a framework composed of a biocompatible, non-living material formed into a three-dimensional structure in a cell culture medium sufficient to meet the nutritional needs required to grow the cells into a stromal tissue, said stromal tissue comprising fibroblast cells and connective tissue proteins naturally secreted by the cells attached to and substantially enveloping the framework, and culturing the cells until the medium contains a desired level of extracellular products so that a conditioned medium is formed;
(b) removing the medium from the cultured cells; and
(c) combining the conditioned medium with a pharmaceutically acceptable carrier to form to the composition. - View Dependent Claims (9, 10, 11)
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Specification