Method of nucleic acid sequencing

• US 6,376,178 B1
• Filed: 11/12/1999
• Issued: 04/23/2002
• Est. Priority Date: 09/03/1993
• Status: Expired due to Fees

• Current Assignee: Duke University
• Original Assignee: Duke University
• Inventors: Sergueev, Dmitri, Porter, Kenneth W., Shaw, Barbara Ramsay
• Primary Examiner(s): Zitomer, Stephanie W.
• Assistant Examiner(s): Wilder, Cynthia B.
• Application Number:
  US09/438,836
• Time in Patent Office: 893 Days
• Field of Search: 435/69.1, 435/6, 435/455, 435/471, 530/350, 536/23.1, 536/25.3, 536/24.5
• US Class Current: 435/6.18
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 2521/319 Exonuclease
• CPC Class Codes: C12Q 2525/113 incorporating modified backbone
• CPC Class Codes: C12Q 2525/125 incorporating agents resulting in resistance to degradation
• CPC Class Codes: C12Q 2525/186 incorporating a non-extendable or blocking moiety
• CPC Class Codes: C12Q 2533/101 Primer extension

Abstract

The present invention relates, in general, to a process of enzymatically synthesizing nucleic acids containing nucleotides that are resistant to degradation. The invention further relates to methods of utilizing such nucleic acids in DNA and RNA amplification and sequencing, gene therapy and molecular detection protocols.

Claims

1. A method of producing a protein comprising introducing into a cell a DNA sequence encoding said protein, which DNA sequence comprises a boronated nucleotide, said introduction being effected under conditions such that said DNA sequence is expressed and said protein thereby produced.

2. A method of producing a protein comprising expressing a DNA sequence encoding said protein, which DNA sequence comprises a boronated nucleotide, said expression being effected under conditions such that said protein is produced.