Electronically-indexed solid-phase assay for biomolecules

• US 6,376,187 B1
• Filed: 07/11/2000
• Issued: 04/23/2002
• Est. Priority Date: 11/30/1995
• Status: Expired due to Fees

• Current Assignee: PharmaSeq, Inc.
• Original Assignee: PharmaSeq, Inc.
• Inventors: Mandecki, Wlodek
• Primary Examiner(s): Chin, Christopher L.
• Application Number:
  US09/614,076
• Time in Patent Office: 651 Days
• Field of Search: 436/501, 436/519, 436/520, 436/824, 436/522, 436/535, 436/518, 436/523, 436/527, 436/533, 435/6, 435/7.1, 435/7.92, 435/7.93, 435/7.94, 435/7.95, 435/810, 435/973, 435/975, 340/825.54, 340/825.06, 340/870.11
• US Class Current: 435/6.19
• CPC Class Codes: B01J 2219/00567 Transponder chips
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C40B 70/00 Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
• CPC Class Codes: G01N 15/14 Optical investigation techniques, e.g. flow cytometry
• CPC Class Codes: G01N 33/54313 the carrier being characterised by its particulate form
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 435/973 Simultaneous determination of more than one analyte
• CPC Class Codes: Y10S 435/975 Kit
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property
• CPC Class Codes: Y10S 436/808 Automated or kit
• CPC Class Codes: Y10S 436/824 Immunological separation techniques

Abstract

Disclosed are materials and methods for detecting biomolecules in samples employing transponders associated with the bead(s) used as the solid phase in the assay, and information pertinent to the assay is encoded on the transponders memory elements. A dedicated read/write device is used remotely to encode or remotely to read the information. The invention can be used in direct or competitive ELISA-type assays, or in multiplex assays for the simultaneous assay of several analytes.

Claims

1. A method of performing an assay for a biomolecule in a sample, the biomolecule being a second member of a biomolecular binding pair, comprising:

(a) providing a solid phase comprising a transponder and a first member of the biomolecular binding pair attached directly or indirectly to an outer surface of the transponder wherein,

(i) the transponder comprises of memory elements, a radio-frequency transmitter and one or more photovoltaic cells,

(ii) the one or more photovoltaic cells provide a source of electrical power for the transponder when illuminated by light, and

(iii) the memory elements contain data indicating the characteristics of the first member of the biomolecular binding pair;

(b) contacting the solid phase with the sample to cause the biomolecule to specifically bind to the first member of the biomolecular binding pair attached to the solid phase;

(c) contacting the solid phase with a fluorescent label reagent, the label reagent specifically binding to the first member of the biomolecular binding pair or, directly or indirectly, to the biomolecule;

(d) analyzing the solid phase with the light to detect any fluorescent label reagent attached; and

(e) decoding the memory elements to determine the characteristics of the first member of the biomolecular binding pair.

2. The method of claim 1, wherein the biomolecular binding pair is an antigen-antibody binding pair.

3. The method of claim 1, wherein the biomolecular binding pair is an nucleic acid and another nucleic acid of a complementary sequence.

4. The method of claim 1, wherein the solid phase is particulate.

5. A method of performing an assay for at least two different biomolecules in a sample, each of the biomolecules being a second member of a biomolecular binding pair, comprising:

(a) providing a solid phase for each biomolecule, the solid phase comprising a transponder and a first member of the biomolecular binding pair, of which the biomolecule is the second member, attached directly or indirectly to an outer surface of the transponder wherein,

(i) each transponder comprises of memory elements, a radio-frequency transmitter and one or more photovoltaic cells,

(ii) the one or more photovoltaic cells provide a source of electrical power for the transponder when illuminated by light, and

(iii) the transponder memory elements contain data indicating the characteristics of the first member of a molecular binding pair attached to the solid phase;

(b) combining the solid phases to form a combined solid phase;

(c) contacting the combined solid phase with the sample to cause the biomolecules to specifically bind to the corresponding first member of the biomolecular binding pair attached to the combined solid phase;

(d) contacting the combined solid phase with a fluorescent label reagent, the label reagent specifically binding to the first members of the biomolecular binding pairs or, directly or indirectly, to the biomolecules attached to the combined solid phase;

(e) analyzing the combined solid phase with the light to detect any fluorescent label reagent attached; and

(f) decoding the memory elements of at least some of the combined solid phase to determine the characteristics of the first member of the biomolecular binding pair attached.

6. The method of claim 5, wherein the biomolecular binding pair is an antigen-antibody binding pair.

7. The method of claim 5, wherein the biomolecular binding pair is an nucleic acid and another nucleic acid of a complementary sequence.

8. The method of claim 5, wherein the solid phase is particulate.