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Methods and compositions for organ decellularization

  • US 6,376,244 B1
  • Filed: 12/29/1999
  • Issued: 04/23/2002
  • Est. Priority Date: 12/29/1999
  • Status: Expired due to Term
First Claim
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1. A method for producing a decellularized kidney scaffold comprising:

  • mechanically agitating an isolated kidney in distilled water to disrupt cell membranes without destroying the interstitial structure of the kidney;

    treating the isolated kidney in an alkaline solution having a detergent at a concentration effective to extract cellular material without dissolving the interstitial structure of the kidney; and

    washing the isolated kidney in distilled water to remove cellular debris without removing the interstitial structure of the kidney until the kidney is substantially free of the cellular material, to thereby produce a decellularized kidney scaffold.

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