Methods and compositions for organ decellularization
First Claim
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1. A method for producing a decellularized kidney scaffold comprising:
- mechanically agitating an isolated kidney in distilled water to disrupt cell membranes without destroying the interstitial structure of the kidney;
treating the isolated kidney in an alkaline solution having a detergent at a concentration effective to extract cellular material without dissolving the interstitial structure of the kidney; and
washing the isolated kidney in distilled water to remove cellular debris without removing the interstitial structure of the kidney until the kidney is substantially free of the cellular material, to thereby produce a decellularized kidney scaffold.
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Abstract
The invention is directed to methods for producing a decellularized organ or part of an organ. A decellularized organ is produced using an isolated organ mechanically agitated to remove cellular membranes surrounding the isolated organ without destroying the interstitial structure of the organ. After the cellular membrane is removed, the isolated organ is exposed to a solubilizing fluid that extracts cellular material without dissolving the interstitial structure of the organ. A washing fluid is used to remove the solubilized components, leaving behind a decellularized organ.
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8 Claims
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1. A method for producing a decellularized kidney scaffold comprising:
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mechanically agitating an isolated kidney in distilled water to disrupt cell membranes without destroying the interstitial structure of the kidney;
treating the isolated kidney in an alkaline solution having a detergent at a concentration effective to extract cellular material without dissolving the interstitial structure of the kidney; and
washing the isolated kidney in distilled water to remove cellular debris without removing the interstitial structure of the kidney until the kidney is substantially free of the cellular material, to thereby produce a decellularized kidney scaffold. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification