Resonant bio-assay device and test system for detecting molecular binding events

• US 6,376,258 B2
• Filed: 01/10/2000
• Issued: 04/23/2002
• Est. Priority Date: 02/02/1998
• Status: Expired due to Fees

• Current Assignee: Mds Sciex
• Original Assignee: Signature BioScience, Inc.
• Inventors: Hefti, John
• Primary Examiner(s): Chin, Christopher L.
• Application Number:
  US09/480,846
• Publication Number:
  US 20020009723A1
• Time in Patent Office: 834 Days
• Field of Search: 435/6, 435/4, 435/7.1, 435/7.92, 435/287.1, 435/287.2, 436/517, 436/518, 436/149, 436/150, 436/151, 436/805, 436/806, 436/524, 436/525
• US Class Current: 436/518
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property

Abstract

Systems and methods are presented for detecting molecular binding events and other environmental effects using the unique dielectric properties of the bound molecular structure or structures. A molecular binding region is coupled along the surface of a signal path. A test signal is propagated along the signal path, whereby the test signal couples to the molecular binding region, and in response, exhibits a signal response.

Claims

1. A bio-assay device configured to detect a molecular binding event between a ligand and an antiligand, the bio-assay device comprising:

(i) a signal path operable to support the propagation of a test signal at one or more frequencies between 10 MHz and 1000 GHz, said signal path comprising;

an electrically conductive transmission line,

a ground element, and

a dielectric layer interposed between said electrically conductive transmission line and said ground element; and

(ii) a molecular binding region electromagnetically coupled to said signal path, said molecular binding region comprising said antiligand operable to bind said ligand;

wherein said signal path and said molecular binding region form part of a resonant structure, wherein said resonant structure has a first resonant frequency f_res1 in the absence of a molecular binding event between said ligand and said antiligand and a second resonant frequency f_res2 in the presence of said molecular binding event, and

wherein said molecular binding region is located between at least a portion of said electrically conductive transmission line and at least a portion of said ground element.

2. The bio-assay device of claim 1, further comprising:

an input port coupled to said signal path, said input port comprising;

a first signal conductor coupled to said transmission line; and

a first ground conductor coupled to said ground element; and

an output port coupled to said signal path, said output port comprising;

a second output signal conductor coupled to said transmission line; and

a second ground conductor coupled to said ground element.

3. The bio-assay device of claim 1, wherein said molecular binding region is formed along at least a portion of said electrically conductive transmission line.

4. The bio-assay device of claim 1, wherein said molecular binding region is formed along at least a portion of said ground element.

5. The bio-assay device of claim 1, wherein said transmission line is physically separated from said ligand.

6. The bio-assay device of claim 1, wherein said test signal comprises a signal at one or more frequencies from 45 MHz to 20 GHz.

7. The bio-assay device of claim 1, wherein said electrically conductive transmission line comprises a derivatized surface and said antiligand is attached to said derivatized surface.

8. A bio-assay test system configured to detect the presence or absence of a molecular binding event between a ligand and an antiligand, the system comprising:

(i) a signal source operable to provide a test signal at one or more frequencies between 10 MHz and 1000 GHz;

(ii) a bio-assay device, comprising;

(a) a signal path operable to support the propagation of said test signal, said signal path comprising;

an electrically conductive transmission line,

a ground element, and

a dielectric layer interposed between said electrically conductive transmission line and said ground element; and

(b) a molecular binding region electromagnetically coupled to said signal path, said molecular binding region comprising said antiligand operable to bind to said ligand; and

(iii) a signal detector coupled to said bio-assay device,

wherein said signal path and said molecular binding region form a resonant structure having a first resonant frequency f_res1 in the absence of a molecular binding event between said ligand and said antiligand and a second resonant frequency f_res2 in the presence of said molecular binding event, and

wherein said molecular binding region is located between at least a portion of said electrically conductive transmission line and at least a portion of said ground element.

9. The bio-assay test system of claim 8, wherein said signal source and said signal detector are included within a vector network analyzer.

10. The bio-assay test system of claim 8, wherein said signal source and said signal detector are included within a scalar network analyzer.

11. The bio-assay test system of claim 8, wherein said signal source and said signal detector are included within a time domain reflectometer.