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Methods and systems for monitoring intracellular binding reactions

  • US 6,379,884 B2
  • Filed: 12/28/2000
  • Issued: 04/30/2002
  • Est. Priority Date: 01/06/2000
  • Status: Expired due to Term
First Claim
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1. A method of detecting intracellular binding interactions, comprising:

  • providing a biological cell having at least a first component of a binding reaction disposed therein;

    contacting the cell with a second component of the binding reaction whereby the second component is internalized within the biological cell, the second component comprising a non-protein molecule, at least one of the first and second components having a fluorescent label associated therewith; and

    determining an amount of binding between the first and second components within the cell by measuring a level of polarized fluorescence emitted from within the biological cell.

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