Multi-valving sample injection apparatus

• US 6,382,035 B1
• Filed: 04/02/2001
• Issued: 05/07/2002
• Est. Priority Date: 04/02/2001
• Status: Expired due to Term

• Current Assignee: Idex Health & Science LLC (RELX PLC)
• Original Assignee: Rheodyne, L.P.
• Inventors: Nichols, Jon A.
• Primary Examiner(s): RAEVIS, ROBERT R
• Application Number:
  US09/824,865
• Time in Patent Office: 400 Days
• Field of Search: 738/637.2, 738/637.3, 738/648.3, 738/648.4, 73/615.5, 73/615.6, 422/103
• US Class Current: 73/863.72
• CPC Class Codes: G01N 30/16 Injection G01N30/24 takes precedence
• CPC Class Codes: G01N 30/20 using a sampling valve
• CPC Class Codes: G01N 30/24 Automatic injection systems
• CPC Class Codes: G01N 35/1097 characterised by the valves valves in general F16K

Abstract

A multi-function valve apparatus for use with a Probe-In-Loop (PIL) architecture sample injection assembly which enables both Partial-Fill and Complete-Fill injections. The rotor element is rotatable about a rotation axis relative the stator between: a Load Position, an Overfill Position, and an Injection Position. In the Load Position, a first bridge channel fluidly couples a metering syringe to the sample loop assembly enabling the probe to aspirate one of a discrete volume of sample into the probe, during a Partial-Fill Mode, and a second volume of sample into the probe, during a Complete-Fill Mode. In the Overfill Position, in the Complete-Fill Mode, a second bridge channel fluidly couples a downstream loop portion to a waste port, and the first bridge channel fluidly couples the metering syringe to an upstream loop portion of the sample loop assembly. This enables the metering pump to dispense the sample from the probe into the downstream loop portion and out the waste port to completely fill the downstream loop portion and the second bridge channel with a substantially precise known volume. In the Injection Position, the second bridge channel fluidly couples the downstream loop portion to the analyzing device to inject the sample into the analyzing device, in either the Partial-Fill Mode or and the Complete-Fill Mode.

Claims

1. A multi-function valve apparatus for use with a Probe-In-Loop (PIL) architecture sample injection assembly, having a metering pump and an injection pump, to direct a Partial-Fill or a Complete-Fill of sample into a sample loop assembly, and to inject the sample from the sample loop into an analyzing device, said sample loop assembly including an upstream loop portion coupled between the valve apparatus and a probe configured to aspirate and dispense the sample into a dock, and a downstream loop portion having an interior volume and coupled between the dock and the valve apparatus, said valve apparatus comprising:

a stator element having a stator face defining a metering port fluidly coupled to the metering pump;

an injection pump port fluidly coupled to the injection pump, a loop upstream port fluidly coupled to the upstream loop portion, a loop downstream port fluidly coupled to the downstream loop portion, an exit port fluidly coupled to the analyzing device and a waste port; and

a rotor including a rotor face in fluid-tight contact against said stator face, said rotor face having a first bridge channel, a second bridge channel defining a discrete volume with the stator face, and a third bridge channel, said rotor face being rotatable about a rotation axis relative the stator between;

a Load Position wherein said first bridge channel fluidly couples the metering port to the sample loop upstream port enabling the probe to aspirate a discrete volume of sample into the probe, during a Partial-Fill Mode, or a second volume of sample into the probe, during a Complete-Fill Mode;

an Overfill Position, in the Complete-Fill Mode, wherein the second bridge channel fluidly couples the sample loop downstream port to the waste port, and the first bridge channel fluidly couples the metering port to the sample loop upstream port enabling the metering pump, when the probe is docked in the dock, to dispense the sample from the probe into the downstream loop portion, out of the downstream port, into the second bridge channel and out the waste port to completely fill the downstream loop portion and the second bridge channel with a substantially precise known volume; and

an Injection Position wherein the second bridge channel fluidly couples the sample loop downstream port to the exit port, and the third bridge channel fluidly couples the injection pump port to the sample loop upstream port enabling the injection pump, when the probe is docked in the dock, to inject the discrete volume of sample, in the Partial-Fill Mode, or the precise known volume of sample, in the Complete-Fill Mode, into the analyzing device.

2. The valve apparatus as defined by claim 1, wherein

said waste port, said upstream port and the exit port lie on a first imaginary circle that is concentric with said rotation axis, and

the ends of said second bridge channel further lying on said imaginary circle such that, in the Overfill Position, one end fluidly connects to the waste port and the other end fluidly connects to the loop downstream port, and such that, in the Injection Position, the other end fluidly connects to the loop downstream port and the one end fluidly connects to the exit port.

3. The valve apparatus as defined by claim 2, wherein

said second bridge channel includes a relatively small transverse cross-sectional area to minimize dispersion.

4. The valve apparatus as defined by claim 3, wherein

said second bridge channel is of a transverse cross-sectional area from about 0.05 mm² to about 0.07 mm².

5. The valve apparatus as defined by claim 2, wherein

said second bridge channel lies in said first imaginary circle.

6. The valve apparatus as defined by claim 2, wherein

said stator face further defines a first communication channel having one end in fluid communication with the exit port and the other end lying on the first imaginary circle such that, in the Injection Position, said other end being in fluid communication with the other end of the second bridge channel.

7. The valve apparatus as defined by claim 6, wherein

said second bridge channel and the first communication channel lie in said first imaginary circle.

8. The valve apparatus as defined by claim 7, wherein

said second bridge channel and said first communication channel have relatively small transverse cross-sectional areas to minimize dispersion.

9. The valve apparatus as defined by claim 6, wherein

said third bridge channel fluidly couples the injection pump port to the exit port enabling the injection pump to purge the analyzing device in a fourth valve position.

10. The valve apparatus as defined by claim 9, wherein

said first communication channel lies in said first imaginary circle,

said injection pump port lies in a second imaginary circle,

said stator face further defines a second communication channel lying in said second imaginary circle and having one end in fluid communication with the injection port, and

said third bridge channel extending radially from said rotational axis and fluidly coupling said first communication channel to said second communication channel to enable the injection pump to purge the analyzing device continuously during rotational movement of the valve apparatus from the Overfill Position, the Load Position and to the fourth valve position.

11. The valve apparatus as defined by claim 10, wherein

in said Injection Position, said third bridge channel fluidly couples said second communication channel to said loop upstream port.

12. The valve apparatus as defined by claim 10, wherein

in a fifth valve position, said third bridge channel fluidly couples said injection pump port to said waste port.

13. The valve apparatus as defined by claim 12, wherein

in the fifth valve position, said first bridge channel fluidly couples said metering port to a pump seals port to fluidly couple the metering pump to the pump seals of the injection pump for cleaning thereof.

14. The valve apparatus as defined by claim 1, wherein

in a fifth valve position, said first bridge channel fluidly couples said metering port to a pump seals port to fluidly couple the metering pump to the pump seals of the injection pump for cleaning thereof.

15. The valve apparatus as defined by claim 14, wherein

said metering port is centrally positioned substantially at said rotational axis, and

said first bridge channel extends radially outward from said rotational axis.

16. The valve apparatus as defined by claim 1, wherein

in the Load Position, said loop downstream port is fluidly sealed from both the waste port and the exit port.

17. The valve apparatus as defined by claim 1, wherein

said metering port is centrally positioned substantially at said rotational axis, and

said first bridge channel extends radially outward from said rotational axis.

18. The valve apparatus as defined by claim 17, wherein

in the Injection Position, said first bridge channel fluidly couples the said metering port to a fluid reservoir port to fluidly couple the metering pump to a fluid reservoir of fluid to fill the metering pump.

19. A Probe-In-Loop (PIL) architecture sample injection assembly comprising:

a rotor valve assembly including a stator element having a stator face defining an injection pump port, a metering port, a loop upstream port, a loop downstream port, an exit port and a waste port, and a rotor element having a rotor face in fluid-tight contact against said stator face, and defining at least one bridge channel;

an injection pump fluidly coupled to the stator injection pump port for supplying mobile phase fluid;

a metering pump fluidly coupled to the stator metering port for supplying or withdrawing metered mobile phase fluid;

an analyzing device fluidly coupled to the stator exit port;

a PIL sample loop assembly having a docking station, a probe, an upstream loop portion fluidly coupling the loop upstream port of the stator element to the probe to aspirate sample into the probe and dispense the aspirated sample from the probe into the docking station, and a downstream loop portion fluidly coupling the docking station to the loop downstream port of the stator element, said downstream loop portion having a discrete interior volume between the docking station and the loop downstream port;

said rotor element being rotatable about a rotation axis to rotate the rotor face relative the stator face between;

a Load Position fluidly coupling the metering port to the sample loop upstream port enabling the probe to aspirate one of a discrete volume of sample into the probe, during a Partial-Fill Mode, and a second volume of sample into the probe, during a Complete-Fill Mode;

an Overfill Position, in the Complete-Fill Mode, fluidly coupling the sample loop downstream port to the waste port, and the metering port to the sample loop upstream port enabling the metering pump, when the probe is docked in the docking station, to dispense the aspirated sample from the probe into the downstream loop portion, and out of the loop downstream port toward the waste port to completely fill the downstream loop portion a substantially precise known volume; and

an Injection Position fluidly coupling the sample loop downstream port to the stator exit port, and the stator injection pump port to the sample loop upstream port enabling the injection pump, when the probe is docked in the docking station, to inject the discrete volume of sample, in the Partial-Fill Mode, or the precise known volume of sample, in the Complete-Fill Mode, into the analyzing device.

20. The sample injection assembly as defined by claim 19, wherein

said rotor face having a first bridge channel wherein, in the Load Position, said first bridge channel fluidly couples the metering port to the sample loop upstream port.

21. The sample injection assembly as defined by claim 20, wherein

said rotor face having a second bridge channel defining a discrete volume with the stator face, wherein, in the Overfill Position the second bridge channel fluidly couples the sample loop downstream port to the waste port, and the first bridge channel fluidly couples the metering port to the sample loop upstream port enabling the metering pump, when the probe is docked in the dock, to further dispense the sample from the probe into the downstream loop portion, out of the loop downstream port, into the second bridge channel and out the waste port to completely fill the downstream loop portion and the second bridge channel with the substantially precise known volume.

22. The sample injection assembly as defined by claim 21, wherein

said rotor face having a third bridge channel wherein, in Injection Position, the second bridge channel fluidly couples the sample loop downstream port to the stator exit port, and the third bridge channel fluidly couples the stator injection pump port to the sample loop upstream port.

23. The sample injection assembly as defined by claim 22, wherein

said waste port, said upstream port and the exit port lie on a first imaginary circle that is concentric with said rotation axis, and

the ends of said second bridge channel further lying on said imaginary circle such that, in the Overfill Position, one end fluidly connects to the waste port and the other end fluidly connects to the loop downstream port, and such that, in the Injection Position, the one end fluidly connects to the loop downstream port and the other end fluidly connects to the exit port.

24. The sample injection assembly as defined by claim 19, wherein

said waste port, said upstream port and the exit port lie on a first imaginary circle that is concentric with said rotation axis, and

the ends of said second bridge channel further lying on said imaginary circle such that, in the Overfill Position, one end fluidly connects to the waste port and the other end fluidly connects to the loop downstream port, and such that, in the Injection Position, the one end fluidly connects to the loop downstream port and the one end fluidly connects to the exit port.

25. The sample injection assembly as defined by claim 24, wherein

said stator face further defines a first communication channel having one end in fluid communication with the exit port and the other end lying on the first imaginary circle such that, in the Injection Position, said other end being in fluid communication with the other end of the second bridge channel of the rotor.

26. The sample injection assembly as defined by claim 25, wherein

said second bridge channel and the first communication channel lie in said first imaginary circle.

27. The sample injection assembly as defined by claim 26, wherein

said second bridge channel and said first communication channel have relatively small transverse cross-sectional areas to minimize dispersion.

28. The sample injection assembly as defined by claim 25, wherein

said third bridge channel fluidly couples the injection pump port to the exit port enabling the injection pump to purge the analyzing device in a fourth valve position.

29. The sample injection assembly as defined by claim 28, wherein

said first communication channel lies in said first imaginary circle,

said injection pump port lies in a second imaginary circle,

said stator face further defines a second communication channel lying in said second imaginary circle and having one end in fluid communication with the injection port, and

said third bridge channel extending radially from said rotational axis and fluidly coupling said first communication channel to said second communication channel to enable the injection pump to purge the analyzing device continuously during rotational movement of the sample injection assembly from the Overfill Position, the Load Position and to the fourth valve position.

30. The sample injection assembly as defined by claim 29, wherein

in a fifth valve position, said third bridge channel fluidly couples said injection pump port to said waste port.

31. The sample injection assembly as defined by claim 22, wherein

in a fifth valve position, said first bridge channel fluidly couples said metering port to a pump seals port to fluidly couple the metering pump to the pump seals of the injection pump for cleaning thereof.

32. The sample injection assembly as defined by claim 31, wherein

said metering port is centrally positioned substantially at said rotational axis, and

said first bridge channel extends radially outward from said rotational axis.

33. The sample injection assembly as defined by claim 22, wherein

said metering port is centrally positioned substantially at said rotational axis, and

said first bridge channel extends radially outward from said rotational axis.

34. The sample injection assembly as defined by claim 33, further including:

a fluid reservoir fluidly coupled to a fluid reservoir port on said stator face, and

in the Injection Position, said first bridge channel fluidly couples said metering port to fluid reservoir port, fluidly coupling the metering pump to the fluid reservoir, to fill the metering pump with fluid therefrom.

35. The sample injection assembly as defined by claim 33, further including:

a wash station adapted for receipt of the tip of the probe therein when unlocked from the dock, and fluidly coupled to a fluid wash port on said stator face, and

in a fourth valve position, said first bridge channel fluidly couples said metering port to the fluid wash port, fluidly coupling the metering pump to the wash station to flow fluid past the tip of the probe therein.

36. The sample injection assembly as defined by claim 28, further including:

a wash station adapted for receipt of the tip of the probe therein when unlocked from the dock, and fluidly coupled to a fluid wash port on said stator face, and

in the fourth valve position, said first bridge channel fluidly couples said metering port to the fluid wash port, fluidly coupling the metering pump to the wash station to flow fluid past the tip of the probe therein.

37. A method of transferring sample from a sample source to an analyzing device using multi-function valve apparatus for use with a Probe-In-Loop (PIL) architecture sample injection assembly to direct a Partial-Fill or a Complete-Fill of the sample into a sample loop assembly, and to inject the sample from the sample loop assembly into the analyzing device, said sample loop assembly including an upstream loop portion coupled between the valve apparatus and a probe configured to aspirate and dispense the sample into a dock, and a downstream loop portion having an interior volume and coupled between the dock and the valve, said method comprising:

providing a stator element having a stator face defining a loop upstream port fluidly coupled to the end of the upstream loop portion, a loop downstream port fluidly coupled to the end of the downstream loop portion, an exit port fluidly coupled to the analyzing device and a waste port;

providing a rotor including a rotor face in fluid-tight contact against said stator face, and having a second bridge channel defining a discrete volume with the stator face, said rotor face being rotatable about a rotation axis relative the stator;

loading a discrete volume of sample through the probe, in a Partial-Fill Mode, and a second volume of sample through the probe, in a Complete-Fill Mode;

in the Complete-Fill Mode when the probe is docked in the dock, fluidly coupling the sample loop downstream port to the waste port through the second bridge channel of the rotor, and dispensing a portion of the sample from the probe though the dock and into the downstream loop portion, out of the downstream port, into the second bridge channel and out the waste port to completely fill the downstream loop portion and the second bridge channel with a substantially precise known volume;

fluidly coupling the sample loop downstream port to the exit port; and

injecting the discrete volume of sample, in the Partial-Fill Mode, or the precise known volume of sample, in the Complete-Fill Mode, into the analyzing device.