Method for transdermal sampling of analytes
First Claim
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1. A method for measuring an analyte in an individual'"'"'s body comprising the steps of:
- a. contacting a region of the skin from the individual with a liposomal composition comprising negatively charged lipids;
b. applying at least one electric pulse to the region of the skin where the liposomal composition has been contacted;
c. applying suction to said region of the skin where the electric pulse has been applied to extract fluid from the individual;
d. measuring the analyte in the extracted fluid, and e. correlating the analyte level in the extracted fluid to the level of the analyte in the individuals body.
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Abstract
The present invention discloses a method for transdermal extraction and measurement of analytes in an individual'"'"'s body comprising the steps of permeabilizing the skin by electroporation in the presence of liposomes comprising negatively charged lipids, and extracting the extracellular fluid through the permeabilized skin by using suction. Analytes may be quantitated in the extracted extracellular fluid.
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Citations
22 Claims
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1. A method for measuring an analyte in an individual'"'"'s body comprising the steps of:
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a. contacting a region of the skin from the individual with a liposomal composition comprising negatively charged lipids;
b. applying at least one electric pulse to the region of the skin where the liposomal composition has been contacted;
c. applying suction to said region of the skin where the electric pulse has been applied to extract fluid from the individual;
d. measuring the analyte in the extracted fluid, and e. correlating the analyte level in the extracted fluid to the level of the analyte in the individuals body. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method for measuring an analyte in an individual'"'"'s body comprising the steps of:
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(a) applying at least one electric pulse in the presence of a liposomal composition to a region of the skin of the individual, wherein the liposomal composition comprises negatively charged lipids;
(b) applying suction to said region of the skin to extract fluid from the individual;
(c) measuring the analyte in the extracted fluid; and
(d) correlating the level of the analyte in the extracted fluid to the level in the individual'"'"'s body. - View Dependent Claims (8, 9, 10, 11, 12)
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13. A method for extracting extracellular fluid through the skin of an individual comprising the steps of:
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(a) placing a negative electrode and appositive electrode on the skin, wherein the negative electrode also contains a reservoir, wherein the reservoir contains a liposomal composition comprising negatively charged lipids;
(b) applying at least one electric pulse through the electrodes;
(c) applying suction to the region of the skin where electric pulse has been applied to collect the extracellular fluid. - View Dependent Claims (14, 15, 16, 17)
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18. A method of detecting the presence of an analyte in an individual'"'"'s body comprising the steps of:
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a. contacting a region of the skin from the individual with a liposomal composition comprising negatively charged lipids;
b. applying at least one electric pulse to the region of the skin where the liposomal composition has been contacted;
c. applying suction to said region of the skin where the electric pulse has been applied to extract fluid from the individual;
d. detecting the presence of the analyte in the extracted fluid, wherein the presence of the analyte in the extracted fluid is an indication of the presence of the analyte in the individual'"'"'s body. - View Dependent Claims (19, 20, 21, 22)
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Specification