Method and device for preparing samples for detecting a nucleotide sequence
First Claim
1. A device for preparing one or more samples for detecting a nucleotide sequence by means of polymerase chain reaction (PCR) where each sample is contacted with an analysis solution, said device comprising a support (1) with at least one cavity (2), a lid (3) which is configured complementary to the cavity (2) and which can be placed on the support (1) such that at least some of the analysis solution taken up by the cavity (2) can be displaced into a gap (2) formed between the cavity (2) and the lid (3) and with a seal (5, 12), arranged adjacent an opening of the cavity (2) for sealing the gap (G), wherein the cavity (2) has a surrounding lateral wall (LW) which widens conically toward the opening and the gap (G) has a width (W) of not more than 1 mm, an electrode (6) being integrally molded into the lid (3) and a primer being bound to an internal face (I) of the lid (3), which faces the cavity (2).
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Abstract
The invention relates to a method for preparing samples for detecting a nucleotide sequence by polymerase chain reaction (PCR), according to which a) an analysis solution is filled into at least one cavity (2) provided for in a support (1); b) a lid (3) configured complementary to the shape of the cavity (2) is placed onto the support (1) in such a way that the analysis solution is pushed at least partly into a gap (S) formed between the cavity (2) and the lid (3); and c) the gap (S) is sealed by means of at least one seal (5, 12) provided for near an opening in the cavity (2).
9 Citations
31 Claims
- 1. A device for preparing one or more samples for detecting a nucleotide sequence by means of polymerase chain reaction (PCR) where each sample is contacted with an analysis solution, said device comprising a support (1) with at least one cavity (2), a lid (3) which is configured complementary to the cavity (2) and which can be placed on the support (1) such that at least some of the analysis solution taken up by the cavity (2) can be displaced into a gap (2) formed between the cavity (2) and the lid (3) and with a seal (5, 12), arranged adjacent an opening of the cavity (2) for sealing the gap (G), wherein the cavity (2) has a surrounding lateral wall (LW) which widens conically toward the opening and the gap (G) has a width (W) of not more than 1 mm, an electrode (6) being integrally molded into the lid (3) and a primer being bound to an internal face (I) of the lid (3), which faces the cavity (2).
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29. A kit for preparing samples for detecting a nucleotide sequence by means of polymerase chain reaction (PCR) where the sample is contacted with an analysis solution, said kit comprising:
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a) a support (1) with at least one cavity (2), b) a lid (3) configured complementary to the cavity (2), said lid being placed onto the support (1) such that at least some of the analysis solution taken up by the cavity (2) can be displaced into a gap (G) formed between the cavity (2) and the lid (3), said lid having an electrode (6) integrally molded therein, and c) an analysis solution comprising at least one first primer. - View Dependent Claims (30, 31)
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Specification