Process for the preparation of mineralized collagen fibrils and their uses as bone substitute material

• US 6,384,196 B1
• Filed: 03/24/1999
• Issued: 05/07/2002
• Est. Priority Date: 03/24/1998
• Status: Expired due to Term

• Current Assignee: Biomet Deutschland GmbH (Zimmer Biomet Holdings, Inc.)
• Original Assignee: Merck Patent GmbH (Merck & Co., Inc.)
• Inventors: Bradt, Jens, Pompe, Wolfgang, Weis, Karl
• Primary Examiner(s): Low, Christopher S. F.
• Assistant Examiner(s): Mohamed, Abdel A.
• Application Number:
  US09/275,397
• Time in Patent Office: 1,140 Days
• Field of Search: 530/356, 530/402, 514/2, 514/21, 514/801, 435/69.1
• US Class Current: 530/356
• CPC Class Codes: A61L 27/425 of phosphorus containing material, e.g. apatite
• CPC Class Codes: C08L 89/06 derived from leather or skin , e.g. gelatin
• CPC Class Codes: D01F 1/10 Other agents for modifying properties
• CPC Class Codes: D01F 4/00 Monocomponent artificial filaments or the like of proteins; Manufacture thereof

Abstract

The invention relates to a process for the preparation of mineralized collagen fibrils, where the fibril formation and mineralization take place in one process step, and to the use thereof as bone substitute material. The collagen is recombinant collagen.

Claims

1. A process for the preparation of mineralized collagen fibrils, comprising mixing an acidic solution of uncrosslinked collagen molecules, wherein the solution comprises a calcium-based mineral, with a neutral buffer solution, wherein mineralization of the fibrils begins at an acid pH, thereby forming the fibrils and mineralizing them in one step,

wherein the collagen recombinant collagen.

2. The process according to claim 1, wherein the fibril formation starts before the mineralization.

3. The process according to claim 1, comprising

a) mixing an acidic collagen solution which comprises dissolved calcium salt with a buffer solution which comprises phosphate and sodium chloride, and

b) incubating these components at a suitable temperature, thereby forming fibrils and mineralizing them with calcium phosphate.

4. The process according to claim 1, wherein said collagen fibrils have a diameter of 20-500 nm.

5. The process according to claim 1, further comprising adding during the process step one or more of the polycarboxylates, polyaspartate, polyglutamate or polyphosphoserine, or adding one or more non-collagenous proteins or phosphoprotein, or adding a mixture thereof.

6. Mineralized collagen fibrils, prepared by the process according to claim 1.

7. A process for the preparation of a composite comprising collagen and calcium phosphate, comprising embedding mineralized collagen fibrils prepared by a process according to claim 1 in a calcium phosphate cement,

wherein the ratio of collagen to calcium phosphate cement is 1:

200 to 1;

1 by weight.

8. The process according to claim 7, wherein the embedding of the mineralized collagen fibrils is not oriented.

9. The process according to claim 7, wherein the oriented embedding of the mineralized collagen fibrils is effected by laminates of oriented fibrils.

10. The process according to claim 7, wherein the calcium phosphate cement is prepared from tetracalcium phosphate, calcium hydrogen phosphate, calcium hydroxyapatite, calcium dihydrogen phosphate, calcium carbonate, or sodium phosphate, or mixtures thereof.

11. A method of producing bone substitute material, comprising preparing mineralized collagen fibrils by mixing an acidic solution of uncrosslinked collagen molecules, wherein the solution comprises a calcium-based mineral, with a neutral buffer solution, wherein mineralization of the fibril begins at an acid pH, thereby forming the fibrils and mineralization of them in a process step, wherein the collagen is recombinant collagen.