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Monolayer and electrode for detecting a label-bearing target and method of use thereof

  • US 6,387,625 B1
  • Filed: 06/19/2000
  • Issued: 05/14/2002
  • Est. Priority Date: 06/27/1995
  • Status: Expired due to Term
First Claim
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1. An electrode, comprising:

  • (a) a substrate having a conductive working surface thereon; and

    (b) a non-conductive self-assembled monolayer on said conductive working surface, said monolayer comprising phosphonate molecules having at the minimum at least one phosphonate group and at least one R1 group, wherein the R1 group is covalently bound to a member of a binding pair, and through which monolayer a transition metal complex can freely move from reactants immobilized on the monolayer to the conductive working surface to transfer electrons to the conductive working surface;

    and wherein the conductive working surface comprises an ITO surface.

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