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Covalent attachment of unmodified nucleic acids to silanized solid phase surfaces

  • US 6,387,626 B1
  • Filed: 08/14/2000
  • Issued: 05/14/2002
  • Est. Priority Date: 06/06/1997
  • Status: Expired due to Fees
First Claim
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1. A method for immobilizing an unmodified nucleic acid molecule to a solid phase, which comprises the steps of:

  • (A) coating said solid phase with a silane and allowing said silane-coated solid phase to cure; and

    (B) coupling unmodified nucleic acid molecules having either a terminal 3′

    OH or a terminal 5′

    OH to said silane-coated solid phase.

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