Photo-induced electron transfer fluorescent sensor molecules
First Claim
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1. A modular fluorescence sensor having the following general formula:
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wherein;
Fl is a fluorophore;
N is a nitrogen atom;
Bd1 and Bd2 are independently selected binding groups, wherein the binding groups are capable of binding an analyte molecule to form a stable 1;
1 complex;
Sp is an aliphatic spacer;
An is an anchor group for attaching the sensor to a solid substrate; and
n, m, x and y are integers, where n=1 or 2, m=1 or 2, and y=1 or 2, x is an integer.
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Abstract
Disclosed is a modular fluorescence sensor having the following general formula:
where Fl is a fluorophore, N is a nitrogen atom, Bd1 and Bd2 are independently selected binding groups, Sp is an aliphatic spacer, and An is an anchor group for attaching the sensor to solid substrates. n=1 or 2, m=1 or 2, x is an integer, and y=1 or 2. The binding groups are capable of binding an analyte molecule to form a stable 1:1 complex. In a preferred embodiment, the Bd1 is R1—B(OH)2 and Bd2 is R2—B(OH)2. R1 and R2 are aliphatic or aromatic functional groups selected independently from each other and B is a boron atom. The present invention also provides methods of synthesizing a modular fluorescence sensor and its use in labeling solid substrates.
50 Citations
28 Claims
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1. A modular fluorescence sensor having the following general formula:
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wherein; Fl is a fluorophore;
N is a nitrogen atom;
Bd1 and Bd2 are independently selected binding groups, wherein the binding groups are capable of binding an analyte molecule to form a stable 1;
1 complex;
Sp is an aliphatic spacer;
An is an anchor group for attaching the sensor to a solid substrate; and
n, m, x and y are integers, where n=1 or 2, m=1 or 2, and y=1 or 2, x is an integer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A modular fluorescence sensor having the following general formula:
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wherein; Fl is a fluorophore;
N is a nitrogen atom and B is a boron atom;
R1 and R2 are aliphatic or aromatic functional groups which allow covalent binding of an analyte to the hydroxyl groups forming a stable 1;
1 complex, wherein R1 and R2 are selected independently from each other and;
An is an anchor group for attaching the sensor to a solid substrate; and
x is an integer. - View Dependent Claims (15, 16, 17, 18, 19, 20)
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21. A modular fluorescence sensor of the following formula:
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22. A composition comprising:
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a modular fluorescence sensor having the following general formula;
wherein; Fl is a fluorophore;
N is a nitrogen atom;
Bd1 and Bd2 are independently selected binding groups, wherein the binding groups are capable of binding an analyte molecule to form a stable 1;
1 complex;
Sp is an aliphatic spacer;
An is an anchor group for attaching the sensor to a solid substrate; and
n, m, x and y are integers, were n=1 or 2, m=1 or 2, and y=1 or 2, x is an integer; and
an analyte bound to the sensor. - View Dependent Claims (23, 24, 25, 26)
wherein; B is a boron atom; and
R1 and R2 are aliphatic or aromatic functional groups which allow covalent binding of an analyte to the hydroxyl groups forming a stable 1;
1 complex, wherein R1 and R2 are selected independently from each other.
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26. The composition of claim 22, wherein the analyte is glucose and the modular fluorescence sensor has the following general formula:
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27. A composition comprising:
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a modular fluorescence sensor having the following general formula;
wherein; Fl is a fluorophore;
N is a nitrogen atom;
B is a boron atom;
R1 and R2 are aliphatic or aromatic functional groups which allow covalent binding of an analyte to the hydroxyl groups forming a stable 1;
1 complex, wherein R1 and R2 are selected independently from each other;
An is an anchor group for attaching the sensor to a solid substrate; and
x is an integer; and
glucose bound to the sensor. - View Dependent Claims (28)
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Specification
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Current AssigneeBaxter Healthcare SA (Baxter International Inc.), Baxter International Inc.
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Original AssigneeBeckman Coulter Incorporated (Danaher Corporation)
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InventorsJames, Tony D., Arimori, Susumu
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Primary Examiner(s)Jones, W. Gary
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Assistant Examiner(s)FORMAN, BETTY J
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Application NumberUS09/729,332Time in Patent Office526 DaysField of Search436/501, 436/172, 436/546, 252/301.16, 252/301.22, 435/183US Class Current435/183CPC Class CodesG01N 33/533 with fluorescent labelY10T 436/143333 Saccharide [e.g., DNA, etc.]
