Method of operating an optical sensor adapted for selective analyte-sensing contact with a plurality of samples
First Claim
1. A method of operating an optical sensor, comprising the steps of:
- (a) providing an optical sensor including;
i) a substrate web of predetermined length, the substrate web being substantially gas impermeable and optically transparent in a predetermined spectral range;
ii) a plurality of elongated sensor stripes extending in parallel, spaced relation along the length of said web, each one of said plurality of sensor stripes adapted for providing an optically discernible response to presence of at least one of a plurality of discrete analytes;
iii) said optical sensor adapted for selective analyte-sensing contact with a plurality of samples, wherein each one of the plurality of samples are selectively superimposable with each one of said plurality of elongated sensor stripes at one of a plurality of discrete sample positions along the lengths thereof;
iv) said optically discernible response being substantially identical at said plurality of discrete sample positions along the length thereof;
v) wherein the plurality of samples comprises at least one unknown sample and at least one calibration sample, the optical sensor adapted for being calibrated upon disposition of the calibration sample in said analyte-sensing contact with said optical sensor at one of said discrete sample positions distinct from that of said at least one unknown sample;
(b) placing the calibration sample in said analyte-sensing contact with the optical sensor at one of said plurality of discrete sample positions along the lengths of the sensor stripes;
(c) measuring optical response of the optical sensor at the one of the plurality of discrete sample positions;
(d) obtaining calibration data utilizing the optical response of the one of the plurality of discrete sample positions;
(e) placing the at least one unknown sample in said analyte-sensing contact with the optical sensor at another of the plurality of discrete sample positions along the lengths of the sensor stripes;
(f) measuring optical response of the other of the plurality of discrete sample positions;
(g) utilizing the calibration data obtained for the one of the plurality discrete sample positions for calibration of the optical response of the other of the plurality of discrete sample positions.
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Accused Products
Abstract
A multiple single use optical sensor includes a series of continuous sensor stripes deposited on a substrate web. At least one sample chamber is adapted to extend transversely across a discrete portion of the series of sensor stripes to facilitate analysis of a sample disposed therein. The sample chamber may be moved, or additional sample chambers provided to enable subsequent measurements of additional samples at unused discrete portions of the sensor stripes. The continuous nature of the sensor stripes provides consistency along the lengths thereof to enable calibration data obtained from one discrete portion of the sensor stripes to be utilized for testing an unknown sample an other discrete portion of the sensor stripes. This advantageously eliminates the need for any particular discrete portion of the sensor stripes to be contacted by more than one sample, for improved sensor performance.
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Citations
15 Claims
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1. A method of operating an optical sensor, comprising the steps of:
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(a) providing an optical sensor including;
i) a substrate web of predetermined length, the substrate web being substantially gas impermeable and optically transparent in a predetermined spectral range;
ii) a plurality of elongated sensor stripes extending in parallel, spaced relation along the length of said web, each one of said plurality of sensor stripes adapted for providing an optically discernible response to presence of at least one of a plurality of discrete analytes;
iii) said optical sensor adapted for selective analyte-sensing contact with a plurality of samples, wherein each one of the plurality of samples are selectively superimposable with each one of said plurality of elongated sensor stripes at one of a plurality of discrete sample positions along the lengths thereof;
iv) said optically discernible response being substantially identical at said plurality of discrete sample positions along the length thereof;
v) wherein the plurality of samples comprises at least one unknown sample and at least one calibration sample, the optical sensor adapted for being calibrated upon disposition of the calibration sample in said analyte-sensing contact with said optical sensor at one of said discrete sample positions distinct from that of said at least one unknown sample;
(b) placing the calibration sample in said analyte-sensing contact with the optical sensor at one of said plurality of discrete sample positions along the lengths of the sensor stripes;
(c) measuring optical response of the optical sensor at the one of the plurality of discrete sample positions;
(d) obtaining calibration data utilizing the optical response of the one of the plurality of discrete sample positions;
(e) placing the at least one unknown sample in said analyte-sensing contact with the optical sensor at another of the plurality of discrete sample positions along the lengths of the sensor stripes;
(f) measuring optical response of the other of the plurality of discrete sample positions;
(g) utilizing the calibration data obtained for the one of the plurality discrete sample positions for calibration of the optical response of the other of the plurality of discrete sample positions. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
placing (b) and measuring (c) are undertaken substantially simultaneously with said steps of placing (e) and measuring (f), respectively.
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4. The method as set forth in claim 1, wherein said step of placing (e), further comprises the step of placing the at least one unknown sample in said analyte-sensing contact with the optical sensor adjacent the at least one of the plurality of discrete sample positions.
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5. The method as set forth in claim 1, wherein:
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said step of placing (b) includes placing a calibration sample at a predetermined number of the plurality of discrete sample positions along the lengths of said sensor stripes; and
said step of obtaining (d) includes obtaining calibration data utilizing the optical response of the predetermined number of the plurality of discrete sample positions.
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6. The method as set forth in claim 1, wherein:
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said step of placing (b) includes placing a calibration sample at at least two of the plurality of discrete sample positions along the lengths of the sensor stripes, the two being disposed on opposite sides of the other of the plurality of discrete sample positions along the lengths of the sensor stripes; and
said step of obtaining (d) includes obtaining calibration data utilizing the optical response of the two of the plurality of discrete sample positions along the lengths of the sensor stripes for calibration of the optical response of the other of the plurality of discrete sample positions along the lengths of the sensor stripes.
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7. The method as set forth in claim 1, further comprising the steps of:
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placing other ones of the plurality of samples in analyte-sensing contact with other ones of the plurality of discrete sample positions along the lengths of the sensor stripes, proximate the at least one of the plurality of discrete sample positions;
measuring optical response of the other ones of the plurality of discrete sample positions; and
utilizing the calibration data obtained for the at least one discrete sample position for calibration of the optical response of the other ones of the plurality of discrete sample positions.
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8. The method as set forth in claim 7, wherein:
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said step of placing (b) includes placing a calibration sample at a predetermined number of the plurality of discrete sample positions along the lengths of said sensor stripes; and
said step of obtaining (d) includes obtaining calibration data utilizing the optical response of the predetermined number of the plurality of discrete sample positions.
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9. The method as set forth in claim 1, wherein:
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said step of providing (a) includes providing an optical sensor assembly including the optical sensor, a plurality of sample chambers superimposed in parallel, spaced relation on said web and being superimposed with said plurality of elongated sensor stripes at a plurality of discrete sample positions along the lengths thereof, wherein each of the plurality of sample chambers is adapted for alternately maintaining individual ones of the plurality of samples in said analyte-sensing contact;
said step of placing (b) includes placing a calibration sample in a first one of the plurality of sample chambers;
said step of measuring (c) includes measuring optical response of the optical sensor at the first one of the plurality of sample chambers;
said step of placing (e) includes placing an unknown sample in a second one of the plurality of sample chambers, the second one of the plurality of sample chambers being disposed adjacent the first one of the plurality of sample chambers;
said step of measuring (f) includes measuring optical response of the optical sensor at the second one of the plurality of sample chambers; and
said step of utilizing (g) includes utilizing the calibration data obtained from the first one of the plurality of sample chambers for calibration of the optical response obtained from the second one of the plurality of sample chambers disposed adjacent thereto.
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10. The method as set forth in claim 9, wherein:
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said step of placing (b) includes placing a calibration sample at a plurality of first ones of the plurality of sample chambers; and
said step of obtaining (d) includes obtaining calibration data utilizing the optical response of the plurality of first ones of the plurality of sample chambers.
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11. The method as set forth in claim 9, wherein:
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said step of placing (b) includes placing a calibration sample at at least two of a plurality of first ones of the plurality of sample chambers, the at least two being disposed on opposite sides of a second one of the plurality of sample chambers; and
said step of obtaining (d) includes obtaining calibration data utilizing the optical response of the at least two of a plurality of first ones for calibration of the optical response of the second one of the plurality of sample chambers.
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12. The method as set forth in claim 11, wherein said step of placing (b) includes placing a calibration sample at at least two sample chambers disposed on opposite sides and adjacent a second one of the plurality of sample chambers.
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13. The method as set forth in claim 9, wherein said steps of
placing (b) and measuring (c) are undertaken substantially simultaneously with said steps of placing (e) and measuring (f), respectively. -
14. The method as set forth in claim 9, wherein:
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said step of placing (b) further includes placing a calibration sample in each of a plurality of first ones of the plurality of sample chambers;
said step of measuring (c) further includes measuring optical response of the optical sensor at each of the plurality of first ones of the plurality of sample chambers;
said step of placing (e) further includes placing an unknown sample in respective second ones of the plurality of sample chambers, each of said second ones of the plurality of sample chambers being disposed adjacent one of said first ones of said plurality of sample chambers;
said step of measuring (f) further includes measuring optical response of the optical sensor at each of the plurality of second ones of said plurality of sample chambers; and
said step of utilizing (g) further includes utilizing the calibration data from each of the first ones to analyze the optical response of the second ones of the plurality of sample chambers disposed adjacent thereto.
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15. The method as set forth in claim 14, wherein said steps of
placing (b) and measuring (c) are undertaken substantially simultaneously, respectively, with the steps of placing (e) and measuring (f), for each pair of adjacent first ones and second ones.
Specification