Devices incorporating filters for filtering fluid samples

• US 6,391,265 B1
• Filed: 08/26/1996
• Issued: 05/21/2002
• Est. Priority Date: 08/26/1996
• Status: Expired due to Term

• Current Assignee: Quidel Cardiovascular, Inc. (Quidel Corporation)
• Original Assignee: Biosite Diagnostics Incorporated (Abbott Laboratories)
• Inventors: Batt, Richard Roger, Buechler, Kenneth Francis
• Primary Examiner(s): ALEXANDER, LYLE
• Application Number:
  US08/704,804
• Time in Patent Office: 2,094 Days
• Field of Search: 422/101, 422/58, 422/61, 436/169, 436/170, 436/177, 210/435, 210/446, 210/496, 210/505, 210/506, 210/508, 210/509
• US Class Current: 422/534
• CPC Class Codes: B01D 61/18 Apparatus therefor
• CPC Class Codes: B01D 61/20 Accessories; Auxiliary operations
• CPC Class Codes: B01D 63/081 Manufacturing thereof
• CPC Class Codes: B01D 65/003 Membrane bonding or sealing
• CPC Class Codes: B01D 69/00 Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
• CPC Class Codes: B01D 69/02 characterised by their properties
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/084 Passive control of flow resistance
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: G01N 1/34 Purifying; Cleaning processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003
• CPC Class Codes: G01N 1/4077 by other techniques involving separation of suspended solids
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

Devices and use thereof, the device comprising a filter and a means for region specific compression of the filter. Alternatively, a single step assay device whereby fluid movement through the device occurs substantially solely due to action of capillary force, the device comprising a filter; a region containing the filter; a fluid access port to the region containing the filter; a means for retarding movement of particles through a peripheral filter surface; a fluid egress port from the region containing the filter; a lateral fluid flow path through the filter connecting the fluid access port to the fluid egress port, whereby sample fluid substantially devoid of particulate matter is released from the filter through the egress port; and, an exit region fluidly connected to the egress port.

Claims

1. A device comprising:

a housing comprising a lid, a base substantially parallel to the lid, and a fluid access port in the lid;

a fluid permeable filter within the housing comprising a region for fluid access fluidly connected to the fluid access port, and a region for fluid egress, wherein the filter comprises a substantially smaller depth dimension in comparison to its length and width dimensions, wherein the length/width plane of the filter is oriented within the housing substantially parallel to the lid and base, and wherein said filter is compressed in one specific region, but not in all regions of said filter, whereby fluid and particulate matter flow in said compressed region is retarded;

a fluid flow path through the filter that is a lateral flow path from the region for fluid access through the filter to the region for fluid egress; and

a fluid egress port in the base or between the lid and base comprising a capillary space fluidly connected to the region of fluid egress from the filter, wherein fluid flows from said filter to said capillary space.

2. The device of claim 1, wherein one or more peripheral surfaces of the filter are sealed in a fluid tight manner.

3. The device of claim 1, further comprising a dead space defined by a peripheral surface of the filter and a region of the device.

4. The device of claim 1, wherein said at least one compressed region of the filter is compressed using fluid-impermeable glue, a sealant, or a pressure adhesive tape.

5. The device of claim 1, wherein said at least one compressed region of the filter is compressed between two or more solid surfaces.

6. The device of claim 1 wherein the capillary space fluidly connected to the fluid egress region comprises a means for producing an assay result.

7. The device of claim 1 further comprising a sample reservoir fluidly connected to the fluid access region of the filter.

8. The device of claim 7 wherein the sample reservoir comprises a capillary space.

9. A method for assaying fluid samples, the method comprising adding fluid to the fluid access port of the device of claim 7.

10. The device of claim 1, wherein movement of any particulate matter in the filter through a peripheral surface of the filter is retarded.

11. The device of claim 10, wherein the filter is sealed in a particle-tight manner on all peripheral surfaces, whereby for a sample comprising particulate matter, any particulate matter in the filter is retained in the filter and any fluid in a capillary space along a peripheral surface of the filter is free of particulate matter.

12. The device of claim 11, wherein the filter is sealed in a liquid-tight manner on all peripheral surfaces.

13. The device of claim 11, wherein the filter is sealed in a fluid-tight manner on all peripheral surfaces.

14. A device comprising:

a housing comprising a lid, a base substantially parallel to the lid, and a fluid access port within the lid;

a filter within the housing capable of separating particulate matter from liquid matter in a fluid sample, said filter comprising a fluid access region fluidly connected to the fluid access port and a fluid egress region, wherein the filter is compressed in one specific region, but not in all regions of said filter, whereby fluid and particulate matter flow in said compressed region is retarded;

a fluid egress port within the base or between the lid and base fluidly connected to the region of fluid egress from the filter; and

a fluid flow path through the filter that is in a lateral path from the fluid access region through the filter to the fluid egress region, wherein fluid flows from said filter to said fluid egress port.

15. The device of claim 14, wherein the lateral flow path has a distance greater than or equal to a transverse flow path distance through the filter.

16. The device of claim 14, wherein particulate matter in a fluid sample is retarded from moving from the filter and into a capillary space external to the filter and adjacent to a peripheral filter surface through the use of a fluid-impermeable glue, a sealant, or a pressure adhesive tape.

17. The device of claim 14, wherein said at least one compressed region of the filter is compressed between two or more solid surfaces.

18. The device of claim 14, wherein said at least one compressed region of the filter is compressed by about 1-50% of the native thickness of the filter.