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Method for using unequal primer concentrations for generating nucleic acid amplification products

  • US 6,391,544 B1
  • Filed: 05/15/1998
  • Issued: 05/21/2002
  • Est. Priority Date: 05/15/1998
  • Status: Expired due to Term
First Claim
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1. In a method for amplifying and detecting a target nucleic acid sequence in a test sample comprising the steps of:

  • (a) forming an amplification mixture comprising a test sample, a first and a second primer sequence, and amplification reagents, (b) amplifying the target sequence to generate copies of the target sequence comprising an amplification product from the first and second primers; and

    (c) detecting the copies of the target sequence as an indication of the presence of the nucleic sequence in the test sample;

    wherein the improvement comprises providing the first primer sequence in 15% to 250% excess over the second primer and wherein a probe is hybridized to the amplification product from the first primer to form a hybrid complex and the hybrid complex is detected as an indication of the presence of the nucleic acid sequence in the test sample.

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