Method to detect Dirofilaria immitis infection
First Claim
1. A method to detect D. immitis infection in a non-adapted host comprising:
- (a) contacting a bodily fluid collected from said host with a formulation comprising a recombinant D. immitis Di33 protein under conditions sufficient to form an immunocomplex between said recombinant D. immitis Di33 protein and anti-Di33 antibodies;
(b) measuring immunocomplex formation between said recombinant D. immitis Di33 protein and anti-Di33 antibodies, if any, in said fluid, wherein the presence of said immunocomplex indicates that said host is or has recently been infected with D. immitis, wherein said recombinant D. imminitis Di33 protein is not cross-reactive with scrum from a host infected with an organism selected from the group consisting of Taenia taeniaeformis, Toxocara cati, and Ancylostoma tubaeforme at 10 weeks post-infection, and wherein said method detects D. immitis infection resulting in the maturation of a single adult heartworm.
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Abstract
The present invention includes a method to detect D. immitis infection in a host animal using a D. immitis Di33 protein to detect anti-D. immitis Di33 antibodies in a bodily fluid of the animal. Also included is a method to detect D. immitis infection in a host animal using a D. immitis anti-Di33 protein to detect Di33 proteins in a bodily fluid of the animal. The present invention also relates to D. immitis detection kits that include either a Di33 protein or an anti-Di33 antibody; such kits also include a composition to detect an immunocomplex between the anti-Di33 antibody and D. immitis Di33 protein. The present invention also includes Di33 proteins, nucleic acid molecules encoding such proteins, as well as recombinant molecules and recombinant cells comprising such nucleic acid molecules, and anti-Di33 antibodies. Also included are methods to produce such proteins, nucleic acid molecules and antibodies.
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Citations
17 Claims
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1. A method to detect D. immitis infection in a non-adapted host comprising:
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(a) contacting a bodily fluid collected from said host with a formulation comprising a recombinant D. immitis Di33 protein under conditions sufficient to form an immunocomplex between said recombinant D. immitis Di33 protein and anti-Di33 antibodies;
(b) measuring immunocomplex formation between said recombinant D. immitis Di33 protein and anti-Di33 antibodies, if any, in said fluid, wherein the presence of said immunocomplex indicates that said host is or has recently been infected with D. immitis, wherein said recombinant D. imminitis Di33 protein is not cross-reactive with scrum from a host infected with an organism selected from the group consisting of Taenia taeniaeformis, Toxocara cati, and Ancylostoma tubaeforme at 10 weeks post-infection, and wherein said method detects D. immitis infection resulting in the maturation of a single adult heartworm. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method to detect D. immitis infection in a non-adapted host animal, said method comprising:
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(a) contacting a bodily fluid collected from said animal with a formulation comprising a recombinant D. immitis Di33 protein under conditions sufficient to form an immunocomplex between said recombinant D. immitis Di33 protein and anti-Di33 IgE antibodies; and
(b) measuring immunocomplex formation between said recombinant D. immitis Di33 protein and anti-Di33 IgE antibodies, if any, in said fluid, wherein the presence of said immunocomplex indicates that said animal is or has recently been infected with D. immitis, wherein said recombinant D. immitis Di33 protein is not cross-reactive with scrum from a host infected with an organism selected from the group consisting of Taenia taeniaeformis, Toxocara cati, and Ancylostoma tubaeforme at 10 weeks post-infection and wherein said method detects D. immitis infection resulting in the maturation of a single adult heartworm. - View Dependent Claims (16, 17)
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Specification