Peptides which mimic candida carbohydrate epitopes and their use in a vaccine

• US 6,391,587 B1
• Filed: 06/17/1999
• Issued: 05/21/2002
• Est. Priority Date: 04/28/1997
• Status: Expired due to Fees

• Current Assignee: Montana Inc.
• Original Assignee: Montana State University
• Inventors: Cutler, Jim E., Glee, Pati M., Granger, Bruce L.
• Primary Examiner(s): DUFFY, PATRICIA ANN
• Application Number:
  US09/334,921
• Time in Patent Office: 1,069 Days
• Field of Search: 514/44, 424/184.1, 536/23.1, 435/320.1, 435/69.1, 435/70.1, 435/71.1, 435/71.2, 435/325, 435/252.3, 435/254.11, 435/69.3, 530/328, 530/300
• US Class Current: 435/69.3
• CPC Class Codes: A61K 2039/505 comprising antibodies
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61K 39/0002 Fungal antigens, e.g. Trichophyton, Aspergillus, Candida
• CPC Class Codes: A61K 9/127 Liposomes
• CPC Class Codes: A61K 9/1271 Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers liposome as conjugate A61K47/6911
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C07K 14/40 from Candida
• CPC Class Codes: C07K 16/14 against material from fungi, algea or lichens
• CPC Class Codes: C07K 7/06 having 5 to 11 amino acids
• CPC Class Codes: C07K 7/08 having 12 to 20 amino acids gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68

Abstract

A composition, pharmaceutical composition, vaccine and method for the treatment of disseminated candidiasis due to infection by C. albicans. The composition includes phosphomannan of C. albicans, peptide mimotopes of phosphomannan epitopes, or polynucleotides encoding the peptide mimotopes. Monoclonal antibodies for use in passive immunization against candida infections are also provided.

Claims

1. An isolated polynucleotide encoding a peptide mimitope that binds to an antibody that binds to the same epitope as monoclonal antibody B6.1, American Type Culture Collection Accession Number HB11925.

2. The isolated polynucleotide of claim 1, wherein the peptide mimitope comprises the sequence WVPPGSWY, which corresponds to residues one through nine of SEQ ID NO:

2.

3. The isolated polynucleotide of claim 1, wherein the peptide mimitope consists of the sequence WVPPGSWY, which corresponds to residues one through nine of SEQ ID NO:

2.

4. The isolated polynucleotide of claim 1, wherein the peptide mimitope comprises the sequence YRQFVTGFW, which corresponds to residues one through nine of SEQ ID NO:

3.

5. The isolated polynucleotide of claim 1, wherein the peptide mimitope consists of the sequence YRQFVTGFW, which corresponds to residues one through nine of SEQ ID NO:

3.

6. The isolated polynucleotide of claim 1, wherein the peptide mimitope comprises the sequence HYKTYGGYW, which corresponds to residues one through nine of SEQ ID NO:

4.

7. The isolated polynucleotide of claim 1, wherein the peptide mimitope consists of the sequence HYKTYGGYW, which corresponds to residues one through nine of SEQ ID NO:

4.

8. The isolated polynucleotide of claim 1, wherein the peptide mimitope comprises the sequence SYLTTGGFW, which corresponds to residues one through nine of SEQ ID NO:

5.

9. The isolated polynucleotide of claim 1, wherein the peptide mimitope consists of the sequence SYLTTGGFW, which corresponds to residues one through nine of SEQ ID NO:

5.

10. The isolated polynucleotide of claim 1, wherein the peptide mimitope comprises the sequence SWYEGLRLI, which corresponds to residues one through nine of SEQ ID NO:

6.

11. The isolated polynucleotide of claim 1, wherein the peptide mimitope consists of the sequence SWYEGLRLI, which corresponds to residues one through nine of SEQ ID NO:

6.

12. The isolated polynucleotide of claim 1, wherein the peptide mimitope comprises SEQ ID NO:

8.

13. The isolated polynucleotide of claim 1, wherein the peptide mimitope consists of SEQ ID NO:

8.

14. An expression vector comprising the polynucleotide of any of claims 1 to 13.

15. An isolated host cell comprising the expression vector of claim 14.

16. A method of recombinantly producing a peptide mimitope comprising the step of incubating the host cell of claim 15, wherein the peptide mimitope is expressed.