Biochip and method for patterning and measuring biomaterial of the same

• US 6,391,625 B1
• Filed: 06/27/2000
• Issued: 05/21/2002
• Est. Priority Date: 06/28/1999
• Status: Expired due to Term

• Current Assignee: LG Electronics, Inc. (LG Corporation)
• Original Assignee: LG Electronics, Inc. (LG Corporation)
• Inventors: Kim, Tae Han, Park, Je Kyun
• Primary Examiner(s): Siew, Jeffrey
• Application Number:
  US09/604,405
• Time in Patent Office: 693 Days
• Field of Search: 435/5, 435/6, 435/7.2, 435/91.1, 435/91.2, 435/287.2, 536/22.1, 536/23.1, 536/24.3, 536/24.31, 536/24.32, 536/24.33, 369/275.1, 369/275.3
• US Class Current: 435/287.2
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/00536 in the shape of disks
• CPC Class Codes: B01J 2219/00572 Chemical means
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00689 using computers
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00729 Peptide nucleic acids [PNA]
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 70/00 : Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
• CPC Class Codes: G01N 33/54373 : involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

A biochip including a DNA chip and a protein chip and a method for patterning and measuring biomaterial of the same are disclosed. The method for patterning a biomaterial of the biochip including a reflecting layer and an active layer on a substrate includes the steps of: rotating the biochip; successively irradiating pulse type laser beams to the rotating biochip to activate predetermined regions of the active layer; and fixing a biomaterial pattern on the activated predetermined regions. A method for measuring the patterned biomaterial includes the steps of: reacting a biomaterial labeled with at least one dye material with the biochip; rotating the biochip reacted with at least one biomaterial; successively irradiating laser beams to the rotating biochip; and detecting and processing light derived from the biochip as a result of reaction of the biomaterial to measure the biomaterial. Thus, the biochip having reliability and high packing density can be fabricated at low cost and the biomaterial of the biochip can be measured at high speed without using expensive equipments.

Claims

1. A biochip comprising:

a substrate;

a reflecting layer formed on the entire surface of the substrate;

an active layer formed on the reflecting layer;

a pattern of bound biomaterial formed on a predetermined region of the active layer; and

a groove region and a land region, wherein the boundary between the groove region and the land region is of wobble form.

2. The biochip of claim 1, wherein the substrate has a round shape.

3. The biochip of claim 1, wherein the substrate is selected from the group consisting of glass, polycarbonate, polytetrafluoroethylene, polystyrene, silicon oxide, and silicon nitride.

4. The biochip of claim 1, wherein the reflecting layer is of gold or aluminum.

5. The biochip of claim 1, wherein the active layer is of a silicon oxide film formed on the reflecting layer, and a reaction material and a photosensitive material sequentially formed on the silicon oxide film.

6. The biochip of claim 1, wherein the biomaterial is selected from the group consisting of peptides, proteins, antibodies, DNA, PNA and enzymes.

7. A method for patterning a biomaterial on a biochip including a reflecting layer and an active layer on a substrate comprising:

(a) rotating the biochip;

(b) successively irradiating pulse type laser beams to the rotating biochip to activate predetermined regions of the active layer, wherein the laser beams comprise a first beam for sensing a track on the biochip to transmit a position signal for activation and a second beam for activating a spot of the biochip in accordance with the position signal of the first beam;

(c) fixing a pattern of bound biomaterial on the activated predetermined regions; and

(d) sequentially repeating steps (b) and (c).

8. The method of claim 7, wherein the biochip is circular and wherein the laser beams are irradiating the biochip while moving from a central portion of the biochip to its outer circumference, or from the outer circumference to the central portion in a straight line.

9. The method of claim 7, further comprising the step of removing residual biomaterials that are not fixed on the predetermined regions after the step (c).

19. The method of claim 7, wherein the substrate includes a groove region and a land region, wherein the boundary between the groove region and the land region is of wobble form.

10. A method for using a biochip for measuring a biomaterial, wherein the biochip includes a reflecting layer and an active layer on a subsrate, and wherein the substrate includes a groove region and a land region, wherein the boundary between the groove region and the land region is of wobble form comprising:

reacting a biomaterial labeled with at least one dye material with the biochip;

rotating the biochip with at least one bound biomaterial;

successively irradiating laser beams to the rotating biochip; and

detecting and processing light derived from the biochip as a result of reaction of the biomaterial to measure the biomaterial.

11. The method of claim 10, wherein the laser beams have different wavelengths in accordance with dye materials labeled in the biomaterial.

12. The method of claim 10, wherein the dye material is of fluorescent material or infrared ray material.

13. A kit for measuring a biomaterial comprising:

(a) a biochip comprising;

(i) a substrate, wherein the substrate includes a groove region and a land region, wherein the boundary between the groove region and the land region is of wobble form

(ii) a reflecting layer formed on the entire surface of the substrate,

(iii) an active layer formed on the reflecting layer, and

(iv) a pattern of bound biomaterial formed on a predetermined region of the active layer, and

(b) means for labeling a biomaterial with at least one dye material.

14. The kit of claim 13, wherein the substrate has a round shape.

15. The kit of claim 13, wherein the substrate is selected from the group consisting of glass, polycarbonate, polytetrafluoroethylene, polystyrene, silicon oxide, and silicon nitride.

16. The kit of claim 13, wherein the reflecting layer is of gold or aluminum.

17. The kit of claim 13, wherein the active layer is of a silicon oxide film formed on the reflecting layer, and a reaction material and a photosensitive material sequentially formed on the silicon oxide film.

18. The kit of claim 13, wherein the biomaterial is selected from the group consisting of peptides, proteins, antibodies, DNA, PNA and enzymes.