Oxidized styrenic polymers for DNA binding

• US 6,391,655 B1
• Filed: 07/29/1998
• Issued: 05/21/2002
• Est. Priority Date: 07/30/1997
• Status: Expired due to Term

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Hersh, Leroy S., Bookbinder, Dana C., Xie, Xinying
• Primary Examiner(s): CEPERLEY, MARY
• Application Number:
  US09/124,352
• Time in Patent Office: 1,392 Days
• Field of Search: 436/531, 435/6, 530/391.1
• US Class Current: 436/531
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

A method of immobilizing aminated DNA to a substrate surface is provided for use in DNA hybridization assays. The surface can be widely employed in a variety of molded laboratory products including multiwell plates, cell culture dishes, and biological containers. The method comprises the steps of providing a polystyrene substrate having a surface content of between 0.3 and 4.0% atomic percent oxygen, and attaching amine modified oligonucleotide to the surface of the substrate. A resultant product comprising a polystyrene substrate having a surface oxygen content of between 0.3 and 4.0% atomic percent, and a plurality of amine modified oligonucleotides directly attached to the surface of the substrate, is also disclosed.

Claims

1. A method of immobilizing DNA to a surface consisting essentially of:

a) providing a styrenic polymer substrate having a surface oxygen content of between 0.2 and 8.0 atomic percent, and

b) attaching at least one aminated DNA molecule directly to the surface of the substrate, said aminated DNA molecule comprising a primary amine attached to a terminal end of a DNA molecule via a linker arm.

2. The method of claim 1 wherein said DNA is an oligonucleotide.

3. The method of claim 1 wherein said DNA is cDNA.

4. The method of claim 1 wherein said styrenic polymer substrate has a surface oxygen content of between 0.3 and 4.0 atomic percent.

5. The method of claim 1 wherein said styrenic polymer substrate has a surface oxygen content of between 0.3 and 2.0 atomic percent.

6. A product for use in chemical and biological analysis consisting essentially of:

a styrenic polymer substrate having a surface oxygen content of between 0.2 and 8.0 atomic percent; and

a plurality of aminated DNA molecules directly attached to the surface of said substrate, said aminated DNA molecule comprising a primary amine attached to a terminal end of a DNA molecule via a linker arm.

7. The product of claim 6 wherein said DNA molecules are oligonucleotides.

8. The product of claim 6 wherein said DNA molecules are cDNA.

9. The product of claim 6 wherein said styrenic polymer substrate has a surface oxygen content of between 0.3 and 4.0 atomic percent.

10. The product of claim 6 wherein said styrenic polymer substrate has a surface oxygen content of between 0.3 and 2.0 atomic percent.

11. The product of claim 6 wherein said substrate is a multiwell plate.

12. The product of claim 6 wherein said substrate is a strip of wells.