Compositions for enhancing delivery of nucleic acids to cells

• US 6,392,069 B2
• Filed: 07/08/1998
• Issued: 05/21/2002
• Est. Priority Date: 01/08/1996
• Status: Expired due to Term

• Current Assignee: Canji Inc. (Merck & Co., Inc.), Schering Corporation (Merck & Co., Inc.)
• Original Assignee: Canji Inc. (Merck & Co., Inc.)
• Inventors: Engler, Heidrun, Nagabhushan, Tattanahalli L., Youngster, Stephen Kenneth
• Primary Examiner(s): Wilson, Michael C.
• Application Number:
  US09/112,074
• Publication Number:
  US 20010006946A1
• Time in Patent Office: 1,413 Days
• Field of Search: 514/44, 424/93.1, 552/509, 536/5
• US Class Current: 552/509
• CPC Class Codes: A61K 31/56 Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
• CPC Class Codes: A61K 31/7008 Compounds having an amino group directly attached to a carbon atom of the saccharide radical, e.g. D-galactosamine, ranimustine
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C07J 41/0061 one of the carbon atoms being part of an amide group
• CPC Class Codes: C07J 9/005 containing a carboxylic function directly attached or attached by a chain containing only carbon atoms to the cyclopenta[a]hydrophenanthrene skeleton
• CPC Class Codes: C12N 15/87 Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Abstract

This invention provides methods and compositions for enhancing transfer of an agent into a cell. The agents can include polypeptides, polynucleotides such as genes and antisense nucleic acids, and other molecules. In some embodiments, the agents are modulating agents that can modulate a cellular activity or function when introduced into the cell. The methods and compositions are useful for introducing agents into individual cells, as well as cells that are present as a tissue or organ.

Claims

1. A compound of Formula III:

2. A compound of Formula IV:

3. A compound of Formula V:

4. A delivery enhancing compound of Formula II:

wherein;

X₁ and X₂ are selected from the group consisting of

and X₃ is a saccharide group.

5. The compound according to claim 4, wherein X₁ and X₂ are both

and X₃ is a glucose group.