Detecting and mapping of inflamed zones in a living tissue

• US 6,393,315 B1
• Filed: 04/05/2000
• Issued: 05/21/2002
• Est. Priority Date: 09/12/1997
• Status: Expired due to Fees

• Current Assignee: Communaute Europeene
• Original Assignee: Communaute Europeene
• Inventors: Whelan, Maurice, Aprahamian, Pierre Marc, Lucia, Alfredo, Miehe, Joseph-Albert, Sowinska, Malgorzata, Heisel, Francine
• Primary Examiner(s): Lateef, Marvin M.
• Assistant Examiner(s): Lin, Jeoyuh
• Application Number:
  US09/508,408
• Time in Patent Office: 776 Days
• Field of Search: 600/310, 600/312, 600/473, 600/476, 382/128, 606/1, 606/2, 606/3, 607/1, 607/88, 607/89, 607/91, 607/9.2, 356/51, 356/345, 356/346, 356/300, 356/402, 356/403, 356/432, 356/213
• US Class Current: 600/476
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/417 the bone marrow

Abstract

A method for detecting and mapping inflamed zones in living tissues and a device for implementing the method. The method includes subjecting the tissues to be analyzed to a luminous excitation with a predetermined spectral domain, acquiring at least the raw fluorescence signal of the porphyrins for a plurality of measurement points, and in determining, for each measurement point, the intensity of the fluorescence for the wavelengths characteristic of endogenous porphyrins.

Claims

1. Process for the detection and mapping of inflamed zones of living tissues, which comprises:

subjecting the tissues to be analyzed to a luminous excitation with a spectral field;

acquiring an unprocessed fluorescent signal of endogenous porphyrins at a plurality of measurement points;

acquiring, for the same tissues to be analyzed, fluorescence signals in the spectral bands centered respectively on about 600 nm and on at least one of about 630 nm and 680-690 nm, and on at least one of about 470 nm and 510-520 nm, for each of the measurement points;

forming for each spectral band a fluorescence image from values of fluorescence densities taken in the spectral band in question for the different measurement points,

using a normalization factor of a given value for images collected at at least one of 470 nm and 510 nm and another normalization factor of a different value for images collected at at least one of 600 nm, 630 nm, and 690 nm, the values of these two factors being defined so as to obtain an optimum gradation of the colors or levels of gray on the final image after processing; and

determining, for each measurement point, the fluorescence intensity for wavelengths characteristic of the endogenous porphyrins.

2. The process according to claim 1, wherein the tissues to be analyzed are from the duodenal-pancreatic region.

3. The process according to claim 1, wherein the fluorescence image or the tissues to be analyzed on which at least one inflamed zone appears is formed by using values of the fluorescence intensity suitable for endogenous porphyrins for the different measurement points.

4. The process according to claim 1, further comprising determining a gradation of the inflammatory character of the zones or lesions by correlation with the fluorescence intensity for the wavelengths characteristic of porphyrins.

5. The process according to claim 1, characterized in that the luminous excitation has a power density of delivered energy at most equal to 0.5 W/cm², and constituted of two wavelengths or two spectral bands, namely one about 590 nm or centered on 590 nm adapted for the excitation of porphyrins and the other about 400 nm or centered on 400 nm, adapted for the excitation of the other endogenous chromophores.

6. The process according to claim 1, further comprising carrying out a normalization of the mean values of the intensities of each image obtained and carrying out a point by point processing of the image or images obtained for the spectral band or bands centered on at least one of about 630 nm and about 680-690 nm by using the data contained in the normalized image obtained for the pass-band centered on 600 nm, and a supplemental processing by using information contained in the image or images obtained for the spectral band or bands centered on at least one of about 470 nm and about 510-520 nm.

7. The process according to claim 6, further comprising carrying out a subtraction point by point, or pixel by pixel, of the normalized image intensities collected at 600 nm, from those of the normalized image or images collected at at least one of 630 and 690 nm.

8. The process according to claim 7, further comprising carrying out, after subtraction of the normalized image intensities collected at 600 nm, a point by point or pixel by pixel division of the image intensities or of the normalized images collected at at least one of 630 and 690 nm, with intensities of the normalized image collected at 470 nm or 510 nm.

9. The process according to claim 7, further comprising carrying out, after subtraction of the normalized image intensities collected at 600 nm, a point by point or pixel by pixel division of the image densities or the normalized images collected at at least one of 630 nm and 690 nm, by intensities of the image obtained by point by point subtraction of the intensities of the normalized image collected at 510 nm from those of the normalized image collected at 470 nm.

10. The process according to claim 6, further comprising making the ratio, pixel to pixel, between the fluorescence image of the endogenous porphyrins:

(I₆₃₀+I₆₈₀−

background noise at 600 nm) and that of a NADPH fluorescence I₄₇₀; and

carrying out a multiplication of the obtained image ratio;

(I₆₃₀+I₆₈₀−

background noise)/I₄₇₀) by the fluorescence image collected at 510-520 nm.

11. Device for the detection and mapping of inflamed zones of living tissues, which comprises:

a luminous excitation unit having an intensity equal to at most 0.5 W/cm², in the spectral bands centered on about 400 nm and on about 590 nm;

a filtering module comprising a set of pass-band filters adapted for the selection of phosphorescences specific to endogenous porphyrins;

means for acquiring unprocessed fluorescent signal of the endogenous porphyrins at a plurality of measurement points;

a unit for detecting and recording images of the fluorescence of the surface of the tissues to be analyzed;

means for acquiring, for the same tissues to be analyzed, fluorescence signals in the spectral bands centered respectively on about 600 nm and on at least one of about 630 nm and 680-690 nm, and on at least one of about 470 nm and 510-520 nm, for each of the measuring points;

means for forming for each spectral band a fluorescence image from values of fluorescence densities taken in the spectral band in question for the different measurement points;

a data processing unit for the point by point or pixel by pixel processing of images collected and the control and management of the assembly of the device, associated with means for storing and editing the images, to detect and map the inflamed zones of the tissues;

said data processing unit using a normalization factor of a given value for images collected at at least one of 470 nm and 510 nm and another normalization factor of a different value for images collected at 600 nm and at at least one of 630 nm and 690 nm, the values of these two factors being defined so as to obtain an optimum gradation of the colors or levels of gray on the final image after processing; and

means for determining, for each measurement point, the fluorescence intensity for wavelengths characteristic of the endogenous porphyrins.

12. The device according to claim 11, wherein the unit for detecting and recording images comprises a digital camera CCD, and the excitation unit, the filtering module and the unit for detecting and recording images, are adapted to be operatively associated with optical means used in endoscopy.

13. The device according to claim 12, wherein the digital camera CCD includes an image intensifier with variable gain.

14. The device according to claim 12, further comprising means for carrying out a normalization of the mean values of the intensities of each image obtained and carrying out a point by point processing of the image or images obtained for the spectral band or bands centered on at least one of about 630 nm and about 680-690 nm by using the data contained in the normalized image obtained for the pass-band centered on 600 nm, and a supplemental processing by using information contained in the image or images obtained for the spectral band or bands centered on at least one of about 470 nm and about 510-520 nm.

15. The device according to claim 14, wherein the data processing unit carries out a subtraction point by point, or pixel by pixel, of the normalized image intensities collected at 600 nm, from those of the normalized image or images collected at at least one of 630 and 690 nm.

16. The device according to claim 15, wherein the data processing unit carries out, after subtraction of the normalized image intensities collected at 600 nm, a point by point or pixel by pixel division of the image intensities or of the normalized images collected at at least one of 630 and 690 nm, with intensities of the normalized image collected at 470 nm or 510 nm.

17. The device according to claim 15, wherein the data processing unit carries out, after subtraction of the normalized image intensities collected at 600 nm, a point by point or pixel by pixel division of the image densities or the normalized images collected at at least one of 630 and 690 nm, by intensities of the image obtained by point by point subtraction of the intensities of the normalized image collected at 510 nm from those of the normalized image collected at 470 nm.

18. The device according to claim 14, wherein the data processing unit effectuates a ratio, pixel to pixel, between the fluorescence image of the endogenous porphyrins:

(I₆₃₀+I₆₈₀−

background noise at 600 nm) and that of a NADPH fluorescence I₄₇₀, and carries out a multiplication of the obtained image ratio;

(I₆₃₀+I₆₈₀−

background noise)/I₄₇₀) by the fluorescence image collected at 510-520 nm.