Sample cassette having utility for histological processing of tissue samples

• US 6,395,234 B1
• Filed: 02/08/2000
• Issued: 05/28/2002
• Est. Priority Date: 02/08/2000
• Status: Expired due to Fees

• Current Assignee: General Data Company, Inc.
• Original Assignee: Triangle Biomedical Sciences, Inc. (General Data Company, Inc.)
• Inventors: McAvinney, Timothy M., Hunnell, Jack E.
• Primary Examiner(s): Savage, Matthew O.
• Application Number:
  US09/499,890
• Time in Patent Office: 840 Days
• Field of Search: 422/101,102,104 436/177 220/4.22,4.23,831-836,840,841
• US Class Current: 422/554
• CPC Class Codes: G01N 1/36 Embedding or analogous mounting of samples

Abstract

A cassette for containment, storage and processing of material samples, having a top member that is slideably attachable to and detachable from the base member with minimal force and relaxed requirement for precision in the alignment operation. The top member is hingeably connected to the base member when attached and is positively locked to the base member in the attached closed position. The top member is easily opened and closed with one hand when attached to the base member, and facilitates high volume sample processing, e.g., of tissue samples for the purpose of histological determinations.

Claims

1. A cassette having utility for holding a material sample, said cassette comprising:

a base member comprising a liquid-permeable bottom surface and a plurality of wall members defining a sample chamber for holding the sample therein, said wall members including side walls, and each of said side walls having a top edge and an inside surface;

a top member adapted to be supported on the base member and latched thereto, covering the sample chamber to retain said sample in the sample chamber when in a closed position, and a pair of protrusions extending outwardly from opposite edges of the top member,

wherein the top member is slideably attachable to and detachable from the base member through a hinge mechanism at said hinged attachment, without deformation of or damage to the hinge mechanism, so that when said top member is attached to and positively locked to the base member at the point of hinged attachment, said top member and said base member are hingedly coupled to each other, wherein said hinge mechanism includes guide channels leading from the top edge of the inside surface of the side walls, and said guide channels receive said pair of protrusions; and

the top member comprises a plurality of latching means at points distant to the point of hinged attachment, whereby said plurality of latching means affix the top member to the base member when in the closed position, so that the top member covers the sample chamber of the base member.

2. The cassette of claim 1, wherein said hinge mechanism comprises means for guiding the top member during its attachment to and detachment from the base member.

3. The cassette of claim 1, wherein said guide channels communicate with locking chambers defined by locking lips in said side walls, whereby said pair of protrusions are translatable through said guide channels into said locking chambers defined by said locking lips.

4. The cassette of claim 3, wherein said top member is pivotable about said pair of protrusions when said protrusions are disposed in said locking chambers defined by said locking lips.

5. The cassette of claim 1, wherein:

the base member is rectangularly shaped and comprises a bottom surface, two parallel side walls, and parallel front and back walls;

the top member is rectangularly shaped;

the hinge mechanism comprises locking chambers to which said guide channels are connected;

wherein said guide channels and locking chambers are voids on the interior surfaces of each of the two parallel side walls at a position proximate to the back wall with said voids being formed by reduction in the thickness of the two side walls, and with each of said locking chambers being defined by an overhanging engagement locking lip; and

wherein said two engagement protrusions are generally cylindrical, each extending outwardly in a transverse direction from a side edge of the top member, perpendicular to the direction of the two parallel side walls, so that when the top member is reposed over the base member and translationally slid toward the back wall thereof, said engagement protrusions travel along the guide channels into the locking chambers, forming a hinged coupling about which the top member is pivotally openable and closeable over the base member.

6. The cassette of claim 5, wherein the bottom surface of said base member comprises a longitudinal reinforcement member, which does not fully extend to the back wall of the base member, thereby providing a mechanical stop for the rear edge of the top member in a fully open position.

7. The cassette of claim 6, wherein the top member is pivotally opened to a maximum extent in a fully open position, said maximum extent being characterized by an angle between the base member and the pivotally opened top member that is greater than about 90°

and less than 180°

.

8. The cassette of claim 7, wherein said maximum extent is characterized by an angle of about 110°

between the base member and the pivotally opened top member.

9. The cassette of claim 1, wherein:

each guide channel communicates with a locking chamber defined by an overhanging locking lip; and

said guide channels and locking chambers are arranged so that said protrusions are translationally slidable along the guide channels into the locking chambers to be lockingly retained in the locking chambers, so that the top member is matably engaged with the base member.

10. The cassette of claim 1, wherein:

each guide channel communicates with a locking chamber defined by an overhanging locking lip; and

said guide channels and locking chambers are arranged so that said protrusions are translationally slidable along the guide channels into the locking chambers to be lockingly retained in the locking chambers, so that the top member is matably engaged with the base member.

11. The cassett of claim 1, wherein the latching means comprises:

a latching hook downwardly depending from an overhanging edge of the top member, and

a latching structure that is matably engageable therewith on one or more wall members of the base member,

whereby said latching hook retains the top member in a closed position over the base member by matably engaging with said latching structure, which requires an application of force to disengage said latching hook from said latching structure to open the top member from the base member.

12. The cassette of claim 11, wherein the top member is deformable, so that the force necessary to effect disengagement of the latching means and opening of the top member is reduced by the simultaneous application of a downward force directed on a central portion of the upper surface of the top member and an upward force applied to the front edge of the top member, thereby pivotally translating the latching hook away from the base member and the latching structure.

13. The cassette of claim 12, wherein the top member is made of plastic material.

14. The cassette of claim 1, further comprising means for positioning the top member above the base member in a predictable and repeatable orientation, when said top member is attached to the base member and in an open position to facilitate introducing samples into or removing samples from the sample chamber, said means comprising a longitudinal element positioned in the sample chamber to provide a mechanical stop for the top member in a fully open position.

15. The cassette of claim 1, wherein the top member and the base member are fabricated from molded plastic.

16. The cassette of claim 1, wherein the base member comprises a front wall having a face that is angled onto which sample identification information can be written and displayed.

17. The cassette of claim 1, wherein the base member and the top member are made of plastic materials.

18. The cassette of claim 1, wherein the top member and the base member have a plurality of apertures formed therein to effect fluid flow through the cassette.

19. A cassette for holding a sample, and comprising:

a base member of generally rectangular shape defining a compartment bounded by front, back, and side walls and a liquid-permeable bottom surface, with each side wall on an interior face thereof having formed therein a guide channel communicating with a locking chamber, wherein said locking chamber is bound by a locking lip; and

a cover member including engagement protrusions engageable with the guide channels of respective side walls and translatable therealong into the locking chamber to position the engagement protrusions in the respective locking chambers so that the cover member is pivotally moveable between open and closed states.

20. A cassette for holding a sample, and comprising:

a base member of generally rectangular shape defining a compartment bounded by front, back, and side walls and a liquid-permeable bottom surface, with each side wall on an interior face thereof having formed therein a guide channel communicating with a locking chamber;

said locking chamber is bound by a locking lip, with a locking slot at a front portion of the base member, and with a latch depression in each side wall at a front portion thereof;

a cover member including (a) engagement protrusions engageable with the guide channels of respective side walls and translatable therealong into the locking chambers to position the engagement protrusions in the respective locking chambers so that the cover member is pivotally moveable between open and closed states, (b) a latch hook downwardly depending at a front portion of the cover member and engageable with the locking slot on the base member, (c) downwardly extending edge members at the sides of the cover member, each having on an outside surface thereof a detent enagageable with a corresponding side wall latch depression of the base member, and (d) a frontally protruding finger tab for application of manual finger pressure in opening and closing of the cover member.