Devices and methods for accessing reaction vessels

• US 6,395,235 B1
• Filed: 08/20/1999
• Issued: 05/28/2002
• Est. Priority Date: 08/21/1998
• Status: Expired due to Fees

• Current Assignee: Biotage AB (Biotage)
• Original Assignee: Argonaut Technologies, Inc.
• Inventors: Kilcoin, Christopher, Wasson, James, Hughes, Jan, Long, Terry
• Primary Examiner(s): Drodge, Joseph W.
• Application Number:
  US09/378,664
• Time in Patent Office: 1,012 Days
• Field of Search: 422/99,100,101,103,104,63,129,131,916,937,938 436/43,47,48,174,180 137/597,883,884 73/863.81
• US Class Current: 422/540
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00283 Reactor vessels with top opening
• CPC Class Codes: B01J 2219/00308 interchangeably mounted in racks or blocks
• CPC Class Codes: B01J 2219/00333 Closures attached to the reactor vessels
• CPC Class Codes: B01J 2219/00351 Means for dispensing and evacuation of reagents
• CPC Class Codes: B01J 2219/00369 in multiple or parallel arrangements
• CPC Class Codes: B01J 2219/00389 Feeding through valves
• CPC Class Codes: B01J 2219/00396 Membrane valves
• CPC Class Codes: B01J 2219/00398 in multiple arrangements
• CPC Class Codes: B01J 2219/00418 using pressure
• CPC Class Codes: B01J 2219/00423 using filtration, e.g. through porous frits
• CPC Class Codes: B01J 2219/00484 by shaking, vibrating or oscillating of the reaction vessels
• CPC Class Codes: B01J 2219/00495 Means for heating or cooling the reaction vessels
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 35/10 Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices G01N35/0099 takes precedence
• CPC Class Codes: Y10T 137/87249 Multiple inlet with multiple outlet
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

Devices and methods are provides for delivering fluids into reaction vessels. The present device is an interface head which allows a user to add reagents and wash solvents to a reaction vessel. Typically, the interface head can engage a plurality of these reaction vessels mounted in a cassette or frame and is adapted to removably engage passages leading into the reaction vessel. The interface head allows a user to manually inject materials into the plurality of passageways in the head which are fluidly coupled to the reaction vessel. In one embodiment, the interface head has a septa valve which opens and closes inlets of the plurality of passageways. The septa valve comprises an elongate member with a septum portion and a plurality of septum ports. The elongate member is slidable between a first position wherein at least one inlet of the passageways in the interface head is sealed by the septum portion and a second position wherein said at least one inlet is aligned with one of the ports to allow delivery of materials from the inlet into the reaction vessel. The septum portion is penetrable by needles and thus allows access for needle/syringe type delivery devices.

Claims

1. A device for use with a reaction vessel, said device comprising:

an interface head having a plurality of passageways therein, where said interface head is adapted to removably engage a passage leading into said reaction vessel;

a septa valve on said interface head comprising an elongate member with a septum portion and a plurality of septum ports, said elongate member slidable between a first position wherein at least one inlet of the passageways in the interface head is sealed by said septum portion and a second position wherein said at least one inlet is aligned with one of said ports to allow delivery of materials from the inlet into the reaction vessel.

2. A device as in claim 1 wherein the interface head further comprises a tubular member coupled to one of said passageways and adapted to engage said passage leading into said reaction vessel.

3. A device as in claim 1 wherein said interface head further comprises a plurality of elastomeric sealing members mounted about said inlet of the passageway in slidable contact with said septa valve.

4. A device as in claim 3 wherein the sealing member comprises an O-ring.

5. A device as in claim 1 wherein said elongate member in said second position aligns said plurality of ports of the septa valve with a plurality of inlets of said passageways in the interface head.

6. A device as in claim 1 wherein said elongate member is slidably mounted within a slot on the interface head, said elongate member having stoppers on a distal end and proximal end of the member to limit the range of motion of the member.

7. A device as in claim 1 wherein the septum comprises a layer of sealing material coupled with a layer of chemically inert and protective material.

8. A device as in claim 1 wherein the septum comprises a layer of silicone coupled to a layer of Teflon®

.

9. A device as in claim 1 wherein the septa valve is slidably mounted on said interface head to simultaneously cover a plurality of said inlets.

10. An interface head for use with a reaction vessel, said interface head comprising:

at least one passageway therein, the passageway having an upper end; and

a septa valve, the septa valve comprising a member having a septum portion and at least one septum port, said member slidable between

a first position wherein said upper end of the passageway is sealed by said septum portion and

a second position wherein said upper end of the passageway is unsealed by said septum port,

said interface head adapted to removably engage a passage leading into said reaction vessel.

11. The interface head of claim 10, further configured to allow the transfer of materials between the passageway and the reaction vessel.

12. The interface head of claim 10, wherein the passageway is configured to deliver material toward the reaction vessel.

13. The interface head of claim 10, wherein the passageway is configured to deliver material away from the reaction vessel.

14. The interface head of claim 10 further comprising a pin that mates with a cap of the reaction vessel.

15. The interface head of claim 10, further comprising a port that delivers material to the passageway.

16. The interface head of claim 10, further comprising a port that vents material away from the passageway.

17. The interface head of claim 10, further comprising at least one guide pin to facilitate alignment of the interface head with a housing containing the reaction vessel.

18. The interface head of claim 10, wherein the septum portion is penetrable by a piercing device.

19. The interface head of claim 10, wherein the passageway is configured to receive an instrument for delivering material into, or extracting material from, the passageway when the septa valve is in the second position.

20. The interface head of claim 10, wherein the interface head comprises aluminum.

21. The interface head of claim 10, wherein the interface head comprises polyphenyl sulfide.

22. The interface head of claim 10, further configured to be manually operated.

23. The interface head of claim 10, further configured to be mounted on a robotic manipulator for automated operation.