Optical chemical/biochemical sensor

• US 6,395,558 B1
• Filed: 08/05/1999
• Issued: 05/28/2002
• Est. Priority Date: 08/29/1996
• Status: Expired due to Fees

• Current Assignee: Zeptosens AG (Bayer AG)
• Original Assignee: Zeptosens AG (Bayer AG)
• Inventors: Duveneck, Gert Ludwig, Kraus, Gerolf, Dubendorfer, Jurg, Kunz, Rino Ernst
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Gakh, Yelena G.
• Application Number:
  US09/242,983
• Time in Patent Office: 1,027 Days
• Field of Search: 436/172 422/82.11,82.05 250/227.14 385/12
• US Class Current: 436/172
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide

Abstract

The invention relates to a planar ATR and evanescently exited luminescence optical sensor platform, consisting of a transducer and a recognition layer, wherein changes in the effective refractive index of the recognition layer are converted into a measurable variable in accordance with the integrated-optical light pointer principle. The invention relates also to the use of the method and to the method itself using the sensor platform, for example in label-free biosensory analysis.

Claims

1. A planar optical sensor platform, comprising a support material (a), and at least one thin waveguiding layer (b), the refractive index of the support material (a) being lower than the refractive index of the waveguiding layer (b), and the sensor platform being provided with a coupling grating for coupling in radiation, characterized in that (1) the coupling grating has a structure which is produced on the support material and reproduced in the waveguiding layer, or the coupling grating has a structure which is produced in the waveguiding layer, (2) a recognition layer (c) is located on the waveguiding layer, and (3) said coupling grating has a grating period that is non-constant perpendicular to the direction of the mode propagation, or (4) said coupling grating has a constant grating period and the layer thickness of the waveguiding layer changes perpendicularly to the direction of propagation of the guided mode.

2. A platform according to claim 1, wherein the support material (a) is transparent.

3. A platform according to claim 1, which has, physically separated from the coupling-in grating, a second coupling grating for coupling out the wave guided in the waveguiding layer.

4. A platform according to claim 3, wherein the coupling-out grating has a grating period that is non-constant perpendicular to the direction of propagation of the guided mode.

5. A platform according to claim 1, wherein the thickness of the waveguiding layer (b) is from 30 to 200 nm.

6. A platform according to claim 3, which has, physically separated from the coupling-in grating, a further coupling grating for coupling out the wave guided in the waveguiding layer, the gratings being located to the right and the left of the coupling-in grating.

7. A platform according to claim 1, wherein the recognition layer contains recognition elements, which are immobilized by hydrophobic absorption or covalent bonding directly on the surface of the waveguiding layer, which is unmodified or chemically modified.

8. A platform according to claim 7, wherein the recognition elements are specific chemical or biochemical binding partners for an analyte.

9. A platform according to claim 8, wherein the recognition elements are selected from the group consisting of antibodies for antigens, binding proteins A or G for immunoglobulins, receptors for ligands, oligonucleotides, and single strands of RNA or DNA for their complementary strands, avidins for biotin, enzymes for enzyme substrates, enzyme cofactors or inhibitors, and lectins for carbohydrates.

10. A platform according to claim 1, wherein the recognition layer consists of a membrane, or a membrane which contains chemical or biochemical recognition elements.

11. A method for an optical determination of an analyte which comprises contacting a recognition layer of the planar optical sensor platform according to claim 1 with a sample, coupling in through the grating light from a light source, and measuring the change of the effective refractive index in the region of a recognition layer by recording the position of light points with a position sensitive detection method.

12. A method according to claim 11, wherein CCD rows or arrays are used for the detection of light points.

13. A method according to claim 11, wherein in addition luminescence radiation, which is guided in the waveguide, is coupled out by a second grating provided in the platform, or that amount of luminescence radiation that is excited in the evanescent field but emitted into the halfspace above or below the platform is fed to a determination system.

14. A method according to claim 11, wherein the sample is a body fluid or constituents thereof, surface water, a soil or plant extract or a liquor from a biological or synthesizing process.

15. A method according to claim 13 for the simultaneous determination of pH and ionic strength.

16. A method according to claim 11, wherein coupling-in of light occurs at an angle between −

1° and

+1°

with respect to the normal of the sensor platform, so that two waves propogate in opposite directions towards right and left of the coupling-in grating.