Diagnostic microarray apparatus

• US 6,395,562 B1
• Filed: 09/04/1998
• Issued: 05/28/2002
• Est. Priority Date: 04/22/1998
• Status: Expired due to Fees

• Current Assignee: California Institute of Technology, Universidad Politecnica De Cataluna
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Kido, Horacio, Hammock, Bruce D., Maquieira, Angel
• Primary Examiner(s): NGUYEN, BAO THUY L
• Application Number:
  US09/148,642
• Time in Patent Office: 1,362 Days
• Field of Search: 422/50,55,64,67,82.05,82.11,99 435/6,7.1,7.5 436/518,523,172,805,809,46,165 204/406
• US Class Current: 436/518
• CPC Class Codes: G01N 33/543 with an insoluble carrier for immobilising immunochemicals
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays
• CPC Class Codes: Y10T 436/112499 with sample on test slide

Abstract

A compact assay system having a solid support has at least one capture binding agent on the support surface. By applying a combination of different binding agents on the support surface, the present invention can conduct multiple chemical reactions on the support solid support to detect analytes of interest. The specific reagents, or capture binding agents, are preferably immobilized on the solid support by means of a computer controlled, miniaturized printing system. Specifically, the reagents can be applied onto the solid support using a commercial available printhead of an ink-jet printer. In addition, the support surface also includes areas adapted to be digitally readable by laser to store information concerning binding between capture agents and analytes. The assay system is useful as a sample array holder for performing a variety of chemical analyses, such as matrix assisted laser desorption ionization mass spectrometry analyses.

Claims

1. An apparatus, useful for detecting at least one analyte of interest, comprising:

a solid support, the solid support having a pair of opposed surfaces with at least one capture binding agent on one of the opposed surfaces and informational surfaces on the other of the opposed surfaces, the at least one capture binding agent being capable of binding with the at least one analyte of interest, the informational surfaces adapted to be digitally readable by laser via a plurality of microscopic pit and land formations when information is carried thereon.

2. The apparatus as in claim 1 wherein the support is a compact disc having information readable or recordable at the informational surfaces.

3. The apparatus as in claim 1 wherein the informational surfaces store information readable by laser concerning binding between the at least one capture binding agent and the at least one analyte of interest.

4. The apparatus as in claim 1 wherein the informational surfaces store information readable by laser by which the presence or quantity of the at least one analyte of interest in a liquid sample is indicated.

5. The apparatus as in claim 1 wherein the at least one capture binding agent is in an array on the one surface.

6. The apparatus as in claim 5 wherein the array on the support is on selectively defined areas.

7. The apparatus as in claim 5 wherein each array has an average dimension of about 100 μ

m.

8. The apparatus as in claim 7 wherein the array is a longitudinally extending line or curve.

9. The apparatus as in claim 1 wherein the at least one capture binding agent is in a selected concentration.

10. The apparatus as in claim 1 wherein the at least one capture binding agent is immobilized on the support as microdots.

11. The apparatus as in claim 10 wherein each microdot has an area of less than about 1 mm².

12. The apparatus as in claim 1 wherein the at least one capture binding agent is an immunoreagent.

13. The apparatus as in claim 1 wherein the at least one capture binding agent is an antigen or an antibody.

14. The apparatus as in claim 1 wherein the support has a plurality of different capture binding agents thereon, the capture binding agents being disposed in spaced apart arrays.

15. The apparatus as in claim 1 wherein the at least one capture binding agent is admixed with a matrix assisted laser desorption ionization mass spectrometry reagent.

16. A sample array holder, useful in performing chemical analyses, comprising:

a disk with opposed surfaces, one surface having microscopic pit and land formations, the pit and land formations carrying digital data, the other surface being substantially planar, an array of capture binding agents capable of binding with analytes of interest being deposited on the planar surface.

17. The sample array holder as in claim 16 wherein the capture binding agents are admixed with a matrix suitable for absorbing ultraviolet in performing matrix assisted laser desorption ionization mass spectrometry.