Computer controlled video microscopy system

• US 6,396,532 B1
• Filed: 10/01/1999
• Issued: 05/28/2002
• Est. Priority Date: 10/01/1999
• Status: Expired due to Fees

• Current Assignee: Hoover, Robert G., Rex Hoover
• Original Assignee: Hoover, Robert G., Rex Hoover
• Inventors: Hoover, Rex, Hoover, Robert G.
• Primary Examiner(s): LEE, Y YOUNG
• Application Number:
  US09/410,749
• Time in Patent Office: 970 Days
• Field of Search: 348/79 359/372,373,379,381,382
• US Class Current: 348/79
• CPC Class Codes: G02B 21/362 Mechanical details, e.g. mountings for the camera or image sensor, housings G02B21/364 takes precedence

Abstract

A microscopy system, comprising a stage for supporting a sample; a means for directing a vertical beam of light through the sample; a means for observing an image carried by the beam of light; and an optical system. The optical system features:

i) a plurality of light-transmitting objective lenses arranged in a linear array on a first plate, including at least a first objective lens positioned in the path of the vertical beam of light and a second objective lens not positioned in the path of the vertical beam of light; and

ii) a lens system adapted to receive the vertical beam of light after it has been transmitted through the first objective lens and focus the vertical beam of light onto the imaging plane of the video camera.

A second plate which is rigidly connected with the lens system is slidably fixed to the first plate by means of linear bearings, allowing the first plate to move along the second plate until the first objective lens moves out of the path of the vertical beam of light and the second objective lens moves into the path of the vertical beam of light. The first plate may be caused to move along the second plate until the first objective lens moves out of the path of the vertical beam of light and the second objective lens moves into the path of the vertical beam of light. Exchange of objective lenses in this fashion is normally carried out under computer control.

Claims

1. A microscopy system, comprising:

a) a stage for supporting a sample;

b) a means for directing a vertical beam of light through the sample;

c) a video camera having a defined imaging plane; and

d) an optical system, comprising;

i) a plurality of light-transmitting objective lenses arranged in a linear array on a first plate, including at least a first objective lens positioned in the path of the vertical beam of light and a second objective lens not positioned in the path of the vertical beam of light; and

ii) a lens system positioned in a tube having an axis which is parallel to the vertical beam of light, said lens system being adapted to receive the vertical beam of light after it has been transmitted through the first objective lens and focus the vertical beam of light onto the imaging plane of the video camera;

e) a second plate, rigidly fixed to the tube containing the lens system, where the first plate is slidably fixed to the second so that the first plate may move along the second plate until the first objective lens moves out of the path of the vertical beam of light and the second objective lens moves in to the path of the vertical beam of light; and

f) a means for moving the first plate along the second plate until the first objective lens moves out of the path of the vertical beam of light and the second objective lens moves into the path of the vertical beam of light under computer control.

2. The system of claim 1, wherein the means for moving the first plate along the second plate comprises:

a) a stepper motor rigidly connected with the second plate;

b) a threaded screw rotated by the stepper motor;

c) a threaded nut driven by the screw, said nut being rigidly connected with the first plate; and

d) a computer;

wherein the computer is adapted to either (a) signal the stepper motor to rotate the screw at a predetermined rate in a forward direction for a period of time sufficient to cause the screw to drive the nut for a distance sufficient to move the first plate from a position in which the first objective lens is positioned in the path of the vertical beam of light to a position in which the second objective lens is positioned in the path of the vertical beam of light;

or (b) signal the stepper motor to rotate the screw at a predetermined rate in a reverse direction for a period of time sufficient to cause the screw to drive the nut for a distance sufficient to move the second plate from a position in which the second objective lens is positioned in the path of the vertical beam of light to a position in which the first objective lens is positioned in the path of the vertical beam of light.

3. The system of claim 1, further comprising:

a memory means adapted to record the image focused onto the imaging plane of the video camera and store the recorded image; and

a means for transmitting the recorded image to a video monitor.

4. The system of claim 1, wherein the means for moving the first plate along the second plate is under computer control.

5. The system of claim 1, further comprising a means for adjusting the focus of the image on the imaging plane of the video camera.

6. The system of claim 5, wherein the focus-adjustment means comprises a means for altering the distance traveled by the vertical light beam to reach the objective lens positioned in the path of the vertical beam of light.

7. The system of claim 6, wherein the focus-adjustment means comprises a rack-and-pinion system, comprising:

a vertical rack mounted on a horizontal support plate;

a rotatable pinion engaging the rack, said pinion being connected with the tube containing the lens system; and

a computer-controlled means for rotating said pinion.

8. The system of claim 5, wherein the focus-adjustment means comprises:

a threaded vertical screw rotatably mounted on a rigid vertical support plate;

a nut driven by the threaded vertical screw, said nut being connected with the tube containing the lens system; and

a means for rotating the vertical screw;

wherein rotation of said vertical screw causes said nut and the objective lens turret attached thereto to move vertically.

9. The system of claim 8, wherein the means for rotating the vertical screw is under computer control.

10. The system of claim 1, wherein the means for supporting a sample is a light-transmitting stage adapted to move the sample in a horizontal plane.

11. The system of claim 10, wherein the stage comprises:

a) a first light-transmitting horizontal support plate;

b) a second light-transmitting horizontal plate mounted on the first support plate, said second plate being adapted to reversibly move horizontally in a first direction under computer control; and

c) a third light-transmitting horizontal plate mounted on the second plate, said third plate being adapted to reversibly move horizontally in a second direction under computer control, said second direction being orthogonal to the first direction.

12. The system of claim 11, wherein the stage additionally comprises:

d) a means for rotating the sample.

13. The system of claim 1, wherein the means for producing a vertical beam of light comprises a second lens system adapted to transmit a beam of light from a light source to a condenser lens system, said condenser lens system being adapted to concentrate the beam of light transmitted from the light source and direct the concentrated beam of light upwards through the stage, said stage being light-transmitting.

14. The system of claim 13, wherein the condenser lens system is a single fixed lens.

15. The system of claim 13, wherein the condenser lens comprises a first lens positioned in the path of the beam of light from the light source, a second lens positioned in the path of the beam of light from the light source between the first fixed lens and the light-transmitting stage, and a means for reversibly moving the second lens out of the path of the beam of light from the light source under computer control.

16. The system of claim 15, wherein the condenser lens system comprises:

a first fixed lens in the path of the beam of light from the light source, said first fixed lens being mounted in a first frame which is adapted to move reversibly in a vertical direction under computer control;

a second lens mounted in a second frame which is rigidly attached to a bar, said bar being movably attached to the rigid frame by means of a hinge;

wherein, when the first frame moves vertically, a shaped surface on the bar interacts with an immobile pin so as to cause the bar to swing from a first bar position where the second lens is positioned in the path of the beam of light from the light source to a second bar position where the second lens is not positioned in the path of the beam of light from the light source.

17. A microscopy system, comprising:

a) a stage for supporting a sample;

b) a means for directing a vertical beam of light through the sample;

c) a means for observing an image carried by the beam of light; and

d) an optical system, comprising;

i) a plurality of light-transmitting objective lenses arranged in a linear array on a first plate, including at least a first objective lens positioned in the path of the vertical beam of light and a second objective lens not positioned in the path of the vertical beam of light; and

ii) a lens system adapted to receive the vertical beam of light after it has been transmitted through the first objective lens and focus the vertical beam of light onto the imaging plane of the video camera;

e) a second plate, rigidly connected with the lens system, where the first plate is slidably fixed to the second plate so that the first plate may move along the second plate until the first objective lens moves out of the path of the vertical beam of light and the second objective lens moves into the path of the vertical beam of light; and

f) a means for moving the first plate along the second plate until the first objective lens moves out of the path of the vertical beam of light and the second objective lens moves into the path of the vertical beam of light under computer control.