Use of wicking agent to eliminate wash steps for optical diffraction-based biosensors
First Claim
1. A method of detecting an analyte in a medium comprising:
- contacting the medium with a sensing device, the sensing device comprising;
a) a polymer film;
b) an analyte-specific receptor layer printed in a pattern onto the polymer film wherein the analyte-specific receptor layer has a receptor material thereon that is specific for the analyte; and
c) a wicking agent on the layer of the analyte-specific receptor layer;
transmitting a light through the wicking agent and the polymer film; and
detecting presence of the analyte by detecting a pattern formed by diffraction of the transmitted light;
wherein the diffraction pattern is caused by binding of the analyte to the patterned analyte-specific receptor layer.
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Accused Products
Abstract
The present invention provides an inexpensive and sensitive system and method for detecting analytes present in a medium. The system comprises a diffraction enhancing element, such as functionalized microspheres, which are modified such that they are capable of binding with a target analyte. Additionally, the system comprises a polymer film, which may include a metal coating, upon which is printed a specific, predetermined pattern of a analyte-specific receptors. Finally, the system includes a wicking agent which permits the system to be a single step system which avoids the necessity of any additional rinsing steps. Upon attachment of a target analyte to select areas of the polymer film, either directly or with the diffraction enhancing element, diffraction of transmitted and/or reflected light occurs via the physical dimensions and defined, precise placement of the analyte. A diffraction image, such as a hologram, is produced which can be easily seen with the eye or, optionally, with a sensing device.
235 Citations
47 Claims
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1. A method of detecting an analyte in a medium comprising:
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contacting the medium with a sensing device, the sensing device comprising;
a) a polymer film;
b) an analyte-specific receptor layer printed in a pattern onto the polymer film wherein the analyte-specific receptor layer has a receptor material thereon that is specific for the analyte; and
c) a wicking agent on the layer of the analyte-specific receptor layer;
transmitting a light through the wicking agent and the polymer film; and
detecting presence of the analyte by detecting a pattern formed by diffraction of the transmitted light;
wherein the diffraction pattern is caused by binding of the analyte to the patterned analyte-specific receptor layer.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A method of detecting an analyte in medium comprising:
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contacting the with a sensing device, the sensing device comprising;
a) a polymer film;
b) an analyte-specific receptor layer printed in a pattern onto the polymer film wherein the analyte-specific receptor layer has a receptor material thereon that is specific for the analyte;
c) a wicking agent on the analyte-specific receptor layer;
reflecting a light source through the wicking agent and off a surface of the metal-coated polymer film; and
detecting presence of the analyte by detecting a pattern formed by diffraction of the reflected light;
wherein the diffraction pattern is caused by binding of the analyte to the patterned analyte-specific receptor layer.- View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47)
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Specification