Rupestris stem pitting associated virus nucleic acids, proteins, and their uses

• US 6,399,308 B1
• Filed: 11/07/2000
• Issued: 06/04/2002
• Est. Priority Date: 05/20/1997
• Status: Expired due to Fees

• Current Assignee: ProfiGen Inc.
• Original Assignee: ProfiGen Inc.
• Inventors: Gonsalves, Dennis, Meng, Baozhong
• Primary Examiner(s): Zitomer, Stephanie W.
• Assistant Examiner(s): Tung, Joyce
• Application Number:
  US09/707,780
• Time in Patent Office: 574 Days
• Field of Search: 435/6, 435/91.2, 536/22.1, 536/24.32, 800/295, 800/301
• US Class Current: 435/6.12
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 15/8283 for virus resistance
• CPC Class Codes: C12N 2770/26022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12Q 1/701 Specific hybridization probes
• CPC Class Codes: Y10S 424/801 involving antibody or fragment thereof produced by recombinant dna technology

Abstract

The present invention relates to an isolated protein or polypeptide corresponding to a protein or polypeptide of a Rupestris stem pitting associated virus. The encoding DNA molecule, either alone in isolated form, in an expression system, a host cell, or a transgenic grape plant, is also disclosed. Other aspects of the present invention relate to a method of imparting Rupestris stem pitting associated virus resistance to grape plants by transforming them with the DNA molecule of the present invention, and a method of detecting the presence of a Rupestris stem pitting associated virus, such as RSPaV-1, in a sample.

Claims

1. Plant material identified according to a method comprising the steps of:

(a) providing a sample from a plant suspected of being infected with Rupestris stem pitting associated virus,

(b) contacting the sample with a nucleic acid probe comprising a Rupestris stem pitting virus nucleic acid molecule under conditions that allow for the hybridization of the probe to a Rupestris stem pitting associated virus nucleic acid molecule, wherein an undetectable hybridization signal indicates that the plant is not infected with a Rupestris stem pitting associated virus, and

(c) selecting a plant that is not infected with a Rupestris stem pitting associated virus.

2. The plant material of claim 1, wherein said plant material is grape plant material.

3. The plant material of claim 2, wherein said grape plant material comprises a rootstock or scion.

4. The plant material of claim 1, wherein said plant material comprises propagated, Rupestris stem pitting associated virus-free material.

5. The plant material of claim 4, wherein said propagated plant material comprises grape plant material.

6. The plant material of claim 5, wherein said grape plant material comprises a rootstock or scion.

7. Grapes produced from the plant material of claim 2.

8. Grapes produced from the propagated plant material of claim 5.