Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction
First Claim
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1. A modified DNA polymerase that is a modified form of a DNA polymerase obtainable from Carboxydothermus hydrogenoformans, wherein said modified DNA polymerasea. exhibits reverse transcriptase activity in the presence of magnesium ions and/or manganese ions;
- b. has reduced or no 5′
-3′
exonuclease activity;
c. has substantially no RnaseH activity;
d. exhibits reverse transcriptase activity in the substantial absence of manganese ions;
e. has a molecular weight of about 64 to about 71 kDa as determined by SDS polyacrylamide electrophoresis; and
f. has amino acid sequence SEQ ID NO;
11.
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Accused Products
Abstract
A purified DNA polymerase exhibiting reverse transcriptase activity in the presence of magnesium ions and/or manganese ions having reduced or no 5′-3′-exonuclease activity and substantially no RNaseH activity and obtainable from Carboxydothermus hydrogenoformans.
110 Citations
5 Claims
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1. A modified DNA polymerase that is a modified form of a DNA polymerase obtainable from Carboxydothermus hydrogenoformans, wherein said modified DNA polymerase
a. exhibits reverse transcriptase activity in the presence of magnesium ions and/or manganese ions; -
b. has reduced or no 5′
-3′
exonuclease activity;
c. has substantially no RnaseH activity;
d. exhibits reverse transcriptase activity in the substantial absence of manganese ions;
e. has a molecular weight of about 64 to about 71 kDa as determined by SDS polyacrylamide electrophoresis; and
f. has amino acid sequence SEQ ID NO;
11.- View Dependent Claims (2)
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- 3. A modified DNA polymerse comprising the amino acid sequence SEQ ID NO:
Specification
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Current AssigneeRoche Diagnostics GmbH (Roche Holding AG)
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Original AssigneeRoche Molecular Systems (Roche Holding AG)
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InventorsEbenbichler, Christine, Ankenbauer, Waltraud, Markau, Ursula, Achhammer, Gunthar
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Primary Examiner(s)Prouty, Rebecca E.
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Assistant Examiner(s)HUTSON, RICHARD G
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Application NumberUS09/204,208Time in Patent Office1,281 DaysField of Search435/91.1, 435/91.2, 435/91.5, 435/194, 435/15, 536/23.2, 536/23.1US Class Current435/15CPC Class CodesC12N 9/1252 DNA-directed DNA polymerase...
