Human gene encoding human chondroitin 6-sulfotransferase

• US 6,399,358 B1
• Filed: 01/29/1998
• Issued: 06/04/2002
• Est. Priority Date: 03/31/1997
• Status: Expired due to Term

• Current Assignee: Columbia University, Thomas Jefferson University (Main Line Health System)
• Original Assignee: Thomas Jefferson University (Main Line Health System)
• Inventors: Tabas, Ira, Williams, Kevin Jon
• Primary Examiner(s): Prouty, Rebecca E.
• Application Number:
  US09/015,188
• Time in Patent Office: 1,587 Days
• Field of Search: 536/23.2, 435/193, 435/320.1, 435/252.3, 435/325
• US Class Current: 435/252.3
• CPC Class Codes: C12N 9/13 transferring sulfur containing groups (2.8)

Abstract

Genes and polypeptides encoded thereby of human chondroitin 6-sulfotransferase are provided. Vectors and host cells comprising these genes and transgenic animals capable of expressing them are also provided. In addition, methods of identifying polymorphic chondroitin 6-sulfotransferase in humans and activators or inhibitors of this enzyme are provided.

Claims

1. A recombinant polynucleolide segment, comprising a polynucleotide sequence, wherein the polynucleotide sequence (a) is SEQ ID NO:

1, or (b) is the full complement of the entire length of SEQ ID NO;

1.

2. A vector comprising the recombinant polynucleotide segment of claim 1.

3. A host cell transformed with the vector claim 2.

4. A recombinant polynucleotide segment comprising a polynucleotide sequence, wherein the polynucleotide sequence is

(a) nucleotides 125 to 1360 of SEQ ID NO:

1;

b) nucleotides 179 to 1360 of SEQ ID NO;

1;

or

(c) the full complement of the entire length of (a) or (b).

5. A vector comprising the recombinant polynucleotide segment of claim 4.

6. A host cell transformed with the vector of claim 5.

7. A recombinant polynucleotide segment, comprising a polynuclcotidc sequence, wherein the polynucleotide sequence (a) encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:

2, or (b) is the full complement of the entire length of a reference sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO;

2.

8. A vector comprising the recombinant polynucleotide segmcnt of claim 7.

9. The vcctor of claim 8, wherein the recombinant polynuclotide segment encodes the polypeptide.

10. A host cell transformed with the vector of claim 8.

11. A host cell transformed with the polynucleotide segment of claim 7.