Up-converting reporters for biological and other assays using laser excitation techniques
First Claim
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1. An apparatus for determining the presence of at least one target analyte in a liquid sample comprising:
- a lateral surface for receiving a liquid sample and transporting the liquid sample to a capture area on the lateral surface, wherein a portion of the lateral surface contains a mobilizable labeled probe comprised of an up-converting phosphor microparticle label attached to a probe which binds specifically to the target analyte to form a labeled probe-analyte complex; and
wherein the capture area has immobilized thereon a capture probe capable of binding specifically to the labeled probe-analyte complex.
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Abstract
The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits radiation at one or more higher frequencies, providing enhanced signal-to-noise ratio and the essential elimination of background sample autofluorescence. The methods, compositions, and apparatus are suitable for the sensitive detection of multiple analytes and for various clinical and environmental sampling techniques.
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Citations
18 Claims
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1. An apparatus for determining the presence of at least one target analyte in a liquid sample comprising:
a lateral surface for receiving a liquid sample and transporting the liquid sample to a capture area on the lateral surface, wherein a portion of the lateral surface contains a mobilizable labeled probe comprised of an up-converting phosphor microparticle label attached to a probe which binds specifically to the target analyte to form a labeled probe-analyte complex; and
wherein the capture area has immobilized thereon a capture probe capable of binding specifically to the labeled probe-analyte complex.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. In an apparatus for determining the presence of at least one target analyte in a liquid sample comprising:
- a lateral surface for receiving a liquid sample and transporting the liquid sample to a capture area on the lateral surface, wherein a portion of the lateral surface contains a mobilizable labeled probe which binds specifically to the target analyte to form a labeled probe-analyte complex; and
wherein the capture area has immobilized thereon a capture probe capable of binding specifically to the labeled probe-analyte complex,the improvement characterized in that the label is an up-converting phosphor label.
- a lateral surface for receiving a liquid sample and transporting the liquid sample to a capture area on the lateral surface, wherein a portion of the lateral surface contains a mobilizable labeled probe which binds specifically to the target analyte to form a labeled probe-analyte complex; and
Specification