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Multiplexed fluorescent detection in microfluidic devices

  • US 6,399,952 B1
  • Filed: 05/12/2000
  • Issued: 06/04/2002
  • Est. Priority Date: 05/12/1999
  • Status: Expired due to Term
First Claim
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1. An optical detection and orientation system for irradiating a fluorescent sample in a microchannel and detecting light emanating from a solid substrate and fluorescence emitted from said channel, wherein said microchannel is in a solid substrate, said system comprising:

  • a movable optical train comprising a source of excitation light and means for directing said light onto said solid substrate;

    means for receiving said light emanating from said solid substrate and transferring said emanating light for analysis; and

    means for moving said optical train and excitation light across the surface of said solid substrate comprising said microchannel and centering said optical train over said microchannel in response to the changes in light emanating from said solid substrate.

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