Method for delivering bioactive agents using cochleates

• US 6,403,056 B1
• Filed: 03/31/2000
• Issued: 06/11/2002
• Est. Priority Date: 03/21/1997
• Status: Expired due to Term

• Current Assignee: Cerevast Medical, Inc.
• Original Assignee: Imarx Therapeutics, Inc. (Sycamore Entertainment Group, Inc.)
• Inventors: Unger, Evan C.
• Primary Examiner(s): Hartley, Michael G.
• Application Number:
  US09/540,448
• Time in Patent Office: 802 Days
• Field of Search: 424/9.52, 424/9.51, 424/450, 424/489, 424/1.21, 424/9.321, 424/812, 424/400, 424/502, 514/937, 514/44
• US Class Current: 424/9.51
• CPC Class Codes: A61K 41/0028 Disruption, e.g. by heat or ultrasounds, sonophysical or sonochemical activation, e.g. thermosensitive or heat-sensitive liposomes, disruption of calculi with a medicinal preparation and ultrasounds
• CPC Class Codes: A61K 47/544 Phospholipids
• CPC Class Codes: A61K 49/223 Microbubbles, hollow microspheres, free gas bubbles, gas microspheres
• CPC Class Codes: A61K 9/1271 Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers liposome as conjugate A61K47/6911
• CPC Class Codes: Y10T 428/2984 Microcapsule with fluid core [includes liposome]

Abstract

The present invention is directed to charged lipids, compositions comprising charged lipids, and the use of these compositions in drug delivery, targeted drug delivery, therapeutic imaging and diagnostic imaging, as well as their use as contrast agents.

Claims

1. A method of delivering a bioactive agent to a patient comprising:

administering to the patient, by intravenous or intra-arterial infusion, a composition comprising cochleate vesicles comprising an anionic lipid, a cationic counter ion, a lipid covalently bonded to a polymer and a bioactive agent; and

applying therapeutic ultrasound to the patient to facilitate delivery of the bioactive agent in a desired region of the patient, wherein said cochleate vesicles are under 2 μ

m in size.

2. The method of claim 1, further comprising imaging the patient to monitor the location of the composition prior to applying the therapeutic ultrasound.

3. The method of claim 1, wherein the anionic lipid is a fluorinated anionic lipid.

4. The method of claim 1, wherein the lipid covalently bonded to the polymer is a fluorinated lipid covalently bonded to the polymer.

5. The method of claim 1, wherein the anionic lipid is selected from the group consisting of a phosphatidic acid, a phosphatidyl glycerol, a phosphatidyl glycerol fatty acid ester, a phosphatidyl ethanolamine anandamide, a phosphatidyl ethanolamine methanandamide, a phosphatidyl serine, a phosphatidyl inositol, a phosphatidyl inositol fatty acid ester, a cardiolipin, a phosphatidyl ethylene glycol, an acidic lysolipid, a sulfolipid, a sulfatide, a saturated free fatty acid, an unsaturated free fatty acid, a palmitic acid, a stearic acid, an arachidonic acid, an oleic acid, a linolenic acid, a linoleic acid, and a myristic acid.

6. The method of claim 1, wherein the cationic counter ion is selected from the group consisting of Be²⁺, Mg²⁺, Ca²⁺, Sr²⁺, Ba²⁺, Al³⁺, Ga³⁺, Ge³⁺, Sn⁴⁺, Pb²⁺, Pb⁴⁺, Ti³⁺, Ti⁴⁺, V²⁺, V³⁺, Cr²⁺, Cr³⁺, Mn²⁺, Mn³⁺, Fe²⁺, Fe³⁺, Co²⁺, Co³⁺, Ni²⁺, Ni³⁺, Cu²⁺, Zn²⁺, Zr⁴+, Nb³+, Mo²⁺, Mo³⁺, Cd²⁺, In³⁺, W²⁺, W⁴⁺, Os²⁺, Os³⁺, Os⁴⁺, Ir²⁺, Ir³⁺, Ir⁴⁺, Hg²⁺, Bi³⁺, La³⁺, and Gd³⁺.

7. The method of claim 6, wherein the cationic counter ion is selected from the group consisting of Ca²⁺, Mg²⁺, Zn²⁺, Mn²⁺ and Gd³⁺.

8. The method of claim 7, wherein the cationic counter ion is Ca²⁺.

9. The method of claim 1, wherein, in the lipid covalently bonded to the polymer, the polymer is selected from the group consisting of polyethylene glycol, polyvinyl pyrrolidone, polyvinyl alcohol, polypropylene glycol, a polyvinylalkylether, a polyacrylamide, a polyalkyloxazoline, a polyhydroxyalkyloxazoline, a polyphosphazene, a polyoxazolidine, a polyaspartamide, a polymer of sialic acid, a polyhydroxyalkyl(meth)acrylate and a poly(hydroxyalkylcarboyxlic acid).

10. The method of claim 9, wherein, in the lipid covalently bonded to the polymer, the polymer is polyethylene glycol.

11. The method of claim 10, wherein the polyethylene glycol has a molecular weight of from about 1,000 to about 10,000.

12. The method of claim 1, wherein the lipid covalently bonded to the polymer is selected from the group consisting of dipalmitoylphosphatidylethanolamine-polyethylene glycol, dioleoylphosphatidylethanolamine-polyethylene glycol and distearylphosphatidylethanolamine-polyethylene glycol.

13. The method of claim 1, wherein the anionic lipid is dipalmitoylphosphatidic acid, the cationic counter ion is Ca²⁺ and the lipid covalently bonded to the polymer is dipalmitoylphosphatidylethanolamine-polyethylene glycol.

14. The method of claim 1, wherein the composition further comprises at least one lipid having a neutral charge.

15. The method of claim 14, wherein the lipid having a neutral charge is a fluorinated lipid having a neutral charge.

16. The method of claim 14, wherein the lipid having a neutral charge is selected from the group consisting of a phosphocholine, a sphingolipid, a glycolipid, a glycosphingolipid, a phospholipid and a polymerized lipid.

17. The method of claim 1, wherein the bioactive agent is genetic material.

18. The method of claim 17, wherein the genetic material is a nucleic acid, RNA, DNA, recombinant RNA, recombinant DNA, antisense RNA, antisense DNA, hammerhead RNA, a ribozyme, a hammerhead ribozyme, an antigene nucleic acid, a ribooligonucleotide, a deoxyribooligonucleotide, or an antisense deoxyribooligonucleotide.

19. The method of claim 1, wherein the bioactive agent is selected from the group consisting of genetic material, a peptide, a beta-agonist, an anti-asthmatic, a steroid, a cholinergic agent, a 5-lipoxygenase inhibitor, a leukotriene inhibitor, an antineoplastic agent, an antibiotic, an anti-tumor drug, and a mitotic inhibitor.

20. The method of claim 1, wherein the composition further comprises a targeting ligand.

21. The method of claim 20, wherein the targeting ligand is selected from the group consisting of peptides, proteins and saccharides.

22. The method of claim 1, wherein the composition further comprises a gas, a gaseous precursor or a gas and a gaseous precursor.

23. The method of claim 22, wherein the gas and gaseous precursor are a fluorinated compound.

24. The method of claim 23, wherein the fluorinated compound is selected from the group consisting of a perflurocarbon, sulfur hexafluoride and a perfluoroether.

25. The method of claim 24, wherein the fluorinated compound is a perfuorocarbon selected from the group consisting of perfluoromethane, perfluoroethane, perfluoropropane, perfluorocyclopropane, perfluorobutane, perfluorocyclobutane, perfluoropentane and perfluorocyclopentane.

26. The method of claim 24, wherein the fluorinated compound is a perfluoroether selected from the group consisting of perfluorotetrahydropyran, perfluoromethyltetrahydrofuran, perfluorobutylmethyl ether, perfluoropropylethyl ether, perfluorocyclobutylmethyl ether, perfluorocyclopropylethyl ether, perfluoropropylmethyl ether, perfluorodiethyl ether, perfluorocyclopropylmethyl ether, perfluoromethylethyl ether and perfluorodimethyl ether.

27. The method of claim 1, wherein the composition further comprises a fluorinated liquid.

28. The method of claim 27, wherein the fluorinated liquid is selected from the group consisting of perfluorohexane, perfluoroheptane, perfluorooctane, perfluorononane, perfluorodecane, perfluorododecane, perfluorocyclohexane, perfluorodecalin, perfluorododecalin, perfluorooctyliodide, perfluorooctylbromide, perfluorotripropylamine, perfluorotributylamine, perfluorobutylethyl ether, bis(perfluoroisopropyl) ether and bis(perfluoropropyl) ether.

61. A method according to claim 1 or 29 wherein said lipid covalently bonded to a polymer is present in an amount from about 5 mole % to about 25 mole %, based on the total amount of lipid in the composition.

62. A method according to claim 1 or 29, wherein said vesicles are under 0.5 μ

m in size.

63. A method according to claim 62, wherein said vesicles are under 200 nm in size.

29. A method of delivering a bioactive agent to a patient comprising:

administering to the patient, by intravenous or intra-arterial infusion, a composition comprising cochleate vesicles comprising a charged lipid, a counter ion, a lipid covalently bonded to a polymer, a bioactive agent, and a targeting ligand, wherein said cochleate vesicles are under 2 μ

m in size.

30. The method of claim 29, further comprising applying therapeutic ultrasound to the patient to facilitate delivery of the bioactive agent in a desired region of the patient.

31. The method of claim 29, further comprising imaging the patient to monitor the location of the composition prior to applying the therapeutic ultrasound.

32. The method of claim 29, wherein the charged lipid is a cationic lipid and the counter ion is an anionic counter ion.

33. The method of claim 32, wherein the cationic lipid is a fluorinated cationic lipid.

34. The method of claim 32, wherein the cationic lipid is selected from the group consisting of phosphatidylethanolamine, phosphatidylcholine, glycero-3-ethyl-phosphatidylcholine, a fatty acyl ester of glycero-3-ethylphosphatidylcholine, dimethyl ammonium propane, trimethyl ammonium propane, diethyl ammonium propane, triethyl ammonium propane, a fatty acyl ester of dimethyl ammonium propane, a fatty acyl ester of trimethyl ammonium propane, a fatty acyl ester of diethyl ammonium propane, a fatty acyl ester of triethyl ammonium propane, N,N′

-Bis (dodecyamino-carbonylmethylene)-N,N′

-bis (β

-N,N,N-trimethylammoniumethylaminocarbonyl-methylene)-ethylenediamine tetraiodide, N,N″

-Bis (hexadecylaminocarbonylmethylene)-N,N′

,N″

-tris (β

-N,N,N-trimethylammoniumethylaminocarbonylmethylenediethylenetriamine hexaiodide, N,N′

-Bis (dodecylaminocarbonylmethylene)-N,N″

-bis(β

-N,N,N-trimethylammoniumethylaminocarbonylmethylene)cyclohexylene-1,4-diamine tetraiodide, 1,1,7,7-tetra-(β

-N,N,N,N-tetramethylammoniumethyl-aminocarbonylmethylene)-3-hexadecylamino-carbonylmethylene- 1,3,7-triaazaheptane heptaiodide, and N,N,N′

N′

-tetra (β

-N,N,N-trimethylammoniumethylamino-carbonylmethylene)-N′

-(1,2-dioleoylglycero-3-phosphoethanolaminocarbonylmethylene)diethylenetriamine tetraiodide.

35. The method of claim 32, wherein the anionic counter ion is selected from the group consisting of ethylene diamine tetraacetic acid, diethylene triamine pentaacetic acid, 1, 4, 7, 10-tetraazocyclododecane-N′

, N′

, N″

, N″

-tetraacetic acid, a dicarboxylic acid, a teraphthalic acid, a sulfide ion, a sulfite ion, a sulfate ion, an oxide ion, a nitride ion, a carbonate ion, a phosphate ion, a polymer of acrylic acid, a copolymer of acrylic acid, a polymer of methacrylic acid, a copolymer of methacrylic acid, and a polymer with at least one pendant SO₃H group.

36. The method of claim 29, wherein the charged lipid is an anionic lipid and the counter ion is a cationic counter ion.

37. The method of claim 36, wherein the anionic lipid is a fluorinated anionic lipid.

38. The method of claim 36, wherein the anionic lipid is selected from the group consisting of a phosphatidic acid, a phosphatidyl glycerol, a phosphatidyl glycerol fatty acid ester, a phosphatidyl ethanolamine anandamide, a phosphatidyl ethanolamine methanandamide, a phosphatidyl serine, a phosphatidyl inositol, a phosphatidyl inositol fatty acid ester, a cardiolipin, a phosphatidyl ethylene glycol, an acidic lysolipid, a sulfolipid, a sulfatide, a saturated free fatty acid, an unsaturated free fatty acid, a palmitic acid, a stearic acid, an arachidonic acid, an oleic acid, a linolenic acid, a linoleic acid, and a myristic acid.

39. The method of claim 36, wherein the cationic counter ion is selected from the group consisting of Be²⁺, Mg²⁺, Ca²⁺, Sr²⁺, Ba²⁺, Al³⁺, Ga³⁺, Ge³⁺, Sn⁴⁺, Pb²⁺, Pb⁴⁺, Ti³⁺, Ti⁴⁺, V²⁺, V³⁺, Cr2+, Cr³⁺, Mn²⁺, Mn³⁺, Fe²⁺, Fe³⁺, Co²⁺, Co³⁺, Ni²⁺, Ni²⁺, Cu²⁺, Zn²⁺, Zr⁴⁺, Nb³⁺, Mo²⁺, Mo³⁺, Cd²⁺, In³⁺, W²⁺, W⁴⁺, Os²⁺, O³⁺, Os⁴⁺, Ir²⁺, Ir³⁺, Ir⁴⁺, Hg²⁺, Bi³⁺, La³⁺, and Gd³⁺.

40. The method of claim 39, wherein the cationic counter ion is selected from the group consisting of Ca²⁺, Mg²⁺, Zn²⁺, Mn²⁺ and Gd³⁺.

41. The method of claim 40, wherein the cationic counter ion is Ca²⁻

.

42. The method of claim 29, wherein the lipid covalently bonded to the polymer is a fluorinated lipid covalently bonded to the polymer.

43. The method of claim 29, wherein, in the lipid covalently bonded to the polymer, the polymer is selected from the group consisting of polyethylene glycol, polyvinyl pyrrolidone, polyvinyl alcohol, polypropylene glycol, a polyvinylalkylether, a polyacrylamide, a polyalkyloxazoline, a polyhydroxyalkyloxazoline, a polyphosphazene, a polyoxazolidine, a polyaspartamide, a polymer of sialic acid, a polyhydroxyalkyl(meth)acrylate and a poly(hydroxyalkylcarboyxlic acid).

44. The method of claim 43, wherein, in the lipid covalently bonded to the polymer, the polymer is polyethylene glycol.

45. The method of claim 44, wherein the polyethylene glycol has a molecular weight of from about 1,000 to about 10,000.

46. The method of claim 29, wherein the lipid covalently bonded to the polymer is selected from the group consisting of dipalmitoylphosphatidylethanolamine-polyethylene glycol, dioleoylphosphatidylethanolamine-polyethylene glycol and distearylphosphatidylethanolamine-polyethylene glycol.

47. The method of claim 36, wherein the anionic lipid is dipalmitoylphosphatidic acid, the cationic counter ion is Ca²⁺ and the lipid covalently bonded to the polymer is dipalmitoylphosphatidylethanolamine-polyethylene glycol.

48. The method of claim 29, wherein the composition further comprises at least one lipid having a neutral charge.

49. The method of claim 48, wherein the lipid having a neutral charge is a fluorinated lipid having a neutral charge.

50. The method of claim 48, wherein the lipid having a neutral charge is selected from the group consisting of a phosphocholine, a sphingolipid, a glycolipid, a glycosphingolipid, a phospholipid and a polymerized lipid.

51. The method of claim 29, wherein the targeting ligand is selected from the group consisting of peptides, proteins and saccharides.

52. The method of claim 29, wherein the bioactive agent is selected from the group consisting of a diagnostic agent, genetic material, a peptide, a beta-agonist, an anti-asthmatic, a steroid, a cholinergic agent, a 5-lipoxygenase inhibitor, a leukotriene inhibitor, an antineoplastic agent, an antibiotic, an anti-tumor drug, and a imitotic inhibitor.

53. The method of claim 52, wherein the bioactive agent is genetic material selected from the group consisting of a nucleic acid, RNA, DNA, recombinant RNA, recombinant DNA, antisense RNA, antisense DNA, hammerhead RNA, a ribozyme, a hammerhead ribozyme, an antigene nucleic acid, a ribooligonucleotide, a deoxyribooligonucleotide, an antisense ribooligonucleotide, and an antisense deoxyribooligonucleotide.

54. The method of claim 29, wherein the composition further comprises a gas, a gaseous precursor or a gas and a gaseous precursor.

55. The method of claim 54, wherein the gas and gaseous precursor are a fluorinated compound.

56. The method of claim 55, wherein the fluorinated compound is selected from the group consisting of a perflurocarbon, sulfur hexafluoride and a perfluoroether.

57. The method of claim 56, wherein the fluorinated compound is a perfluorocarbon selected from the group consisting of perfluoromethane, perfluoroethane, perfluoropropane, perfluorocyclopropane, perfluorobutane, perfluorocyclobutane, perfluoropentane and perfluorocyclopentane.

58. The method of claim 56, wherein the fluorinated compound is a perfluoroether selected from the group consisting of perfluorotetrahydropyran, perfluoromethyltetrahydrofuran, perfluorobutylmethyl ether, perfluoropropylethyl ether, perfluorocyclobutylmethyl ether, perfluorocyclopropylethyl ether, perfluoropropylmethyl ether, perfluorodiethyl ether, perfluorocyclopropylmethyl ether, perfluoromethylethyl ether and perfluorodimethyl ether.

59. The method of claim 29, wherein the composition further comprises a fluorinated liquid.

60. The method of claim 59, wherein the fluorinated liquid is selected from the group consisting of perfluorohexane, perfluoroheptane, perfluorooctane, perfluorononane, perfluorodecane, perfluorododecane, perfluorocyclohexane, perfluorodecalin, perfluorododecalin, perfluorooctyliodide, perfluorooctylbromide, perfluorotripropylamine, perfluorotributylamine, perfluorobutylethyl ether, bis(perfluoroisopropyl) ether and bis(perfluoropropyl) ether.