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Method and apparatus for DNA sequencing and DNA identification

  • US 6,403,315 B1
  • Filed: 02/14/2000
  • Issued: 06/11/2002
  • Est. Priority Date: 12/09/1994
  • Status: Expired due to Fees
First Claim
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1. A method for analyzing a target nucleic acid by hybridization, comprising the steps of:

  • contacting an array of probes fixed on a substrate with the target nucleic acid under hybridization conditions discriminating between full complementarity and a one base mismatch, the fixed probes being shorter than the target nucleic acid, wherein a horizontal electrical field is applied to the array, parallel to the substrate, during at least a portion of said contacting step;

    exposing the array of probes and the target nucleic acid with a labeled probe under hybridization conditions discriminating between full complementarity and a one base mismatch;

    covalently joining fixed and labeled probes that hybridize contiguously to the same target nucleic acid molecule; and

    analyzing the result.

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