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Microfluidic systems and methods of genotyping

DC
  • US 6,403,338 B1
  • Filed: 06/27/2000
  • Issued: 06/11/2002
  • Est. Priority Date: 04/04/1997
  • Status: Expired due to Term
First Claim
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1. A method of genotyping a sample material in a microscale device, the method comprising:

  • flowing a sample comprising a nucleic acid into a microscale chamber or channel in the microscale device, the microscale chamber or channel having at least one reaction region having a cross sectional dimension of between about 0.1 μ

    m and about 500 μ

    m;

    amplifying a plurality of sequences from the sample in the reaction region of the device to form an amplified sample; and

    detecting a plurality of polymorphisms in the amplified sample.

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