Integrated portable biological detection system
First Claim
1. An integrated system for the analysis of eukaroytic and/or prokaryotic cells in a biological sample comprising:
- a. an electronic system for cell separation, cell lysis, sample preparation, and sample analysis further comprising;
at least one input and at least one output port connected to a flow cell, a plurality of individually addressable electrodes positioned within said flow cell wherein each electrode is capable of being electrically biased separately from the other electrodes, and a heating element connected to said flow cell, said flow cell adapted to receive said sample via the input, said electrodes being operatively coupled to a power source adapted to electronically disrupt cell membranes within said sample, said electrodes further comprising an active matrix array adapted to bind to predetermined molecules of interest within said sample for analysis of said molecules;
b. a detector operatively positioned to detect said molecules bound to said active matrix array by a detectable signal; and
c. a power source.
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Accused Products
Abstract
We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.
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Citations
139 Claims
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1. An integrated system for the analysis of eukaroytic and/or prokaryotic cells in a biological sample comprising:
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a. an electronic system for cell separation, cell lysis, sample preparation, and sample analysis further comprising;
at least one input and at least one output port connected to a flow cell, a plurality of individually addressable electrodes positioned within said flow cell wherein each electrode is capable of being electrically biased separately from the other electrodes, and a heating element connected to said flow cell, said flow cell adapted to receive said sample via the input, said electrodes being operatively coupled to a power source adapted to electronically disrupt cell membranes within said sample, said electrodes further comprising an active matrix array adapted to bind to predetermined molecules of interest within said sample for analysis of said molecules;
b. a detector operatively positioned to detect said molecules bound to said active matrix array by a detectable signal; and
c. a power source. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44)
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45. An integrated system for the analysis of a biological sample containing eukaryotic and/or prokaryotic cells, comprising:
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a. a first electronic system for sample preparation, said system further comprising;
at least one input port, a first flow cell connected to the input, a plurality of electrodes positioned within said flow cell wherein each electrode is capable of being electrically biased separately from the other electrodes, and at least one output port, said flow cell adapted to receive said sample via the input, said electrodes being operatively coupled to a power source adapted to electronically disrupt cell membranes within said sample;
b. a second electronic system for sample analysis, said system further comprising;
at least one input port, a second flow cell connected to the input, a plurality of electrodes positioned within said second flow cell wherein each electrode is capable of being electrically biased separately from the other electrodes, said electrodes comprising an active matrix array adapted to bind to predetermined molecules of interest within said sample for analysis of said molecules, and at least one output port, said input of the second electronic system being coupled to at least one output port of the first electronic system;
c. a detector operatively positioned to detect said molecules bound to said active matrix array by a detectable signal; and
d. a power source. - View Dependent Claims (46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94)
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95. An integrated system for the analysis of a biological sample containing eukaroytic and/or prokaryotic cells, comprising:
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a. a first nonelectronic system for sample preparation, said system further comprising;
at least one input port, a first flow cell connected to the input, a heater element, and at least one output port, said flow cell adapted to receive said sample via the input, said heater being operatively coupled to a power source adapted to heat said flow cell to a temperature sufficient to induce disruption of cell membranes within said sample;
b. a second electronic system for sample analysis, said system further comprising;
at least one input port, a second flow cell connected to the input, a plurality of electrodes positioned within said second flow cell wherein each electrode is capable of being electrically biased separately from the other electrodes, said electrodes comprising an active matrix array adapted to bind to predetermined molecules of interest within said sample for analysis of said molecules, and at least one output port, said input of the second electronic system being coupled to at least one output port of the first nonelectronic system;
c. a detector operatively positioned to detect said molecules bound to said active matrix array by a detectable signal; and
d. a power source. - View Dependent Claims (96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139)
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Specification