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Determination of light absorption pathlength in a vertical-beam photometer

  • US 6,404,501 B1
  • Filed: 09/27/2000
  • Issued: 06/11/2002
  • Est. Priority Date: 07/25/1994
  • Status: Expired due to Term
First Claim
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1. A photometric method for determining an optical pathlength of a sample having an anaylte dissolved or suspended in a solvent in the wells of a multiassay plate wherein a relationship between light absorption of the analyte and the optical pathlength of the same are unknown, the method comprising:

  • measuring a first light signal resulting from transmission of light, having a first predetermined wavelength, vertical through the sample, and measuring a second light signal resulting from transmission of light, having a second predetermined wavelength, vertically through the sample and determining the optical pathlength of the sample from a predetermined relationship between the first and second light signal, and the optical pathlength of the solvent, wherein the first and second wavelength are in a near-infrared region of an electromagnetic spectrum of from 750 nanometers to 2500 nanometers wavelength.

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