Time-resolved optoacoustic method and system for noninvasive monitoring of glucose

• US 6,405,069 B1
• Filed: 10/06/1999
• Issued: 06/11/2002
• Est. Priority Date: 01/31/1996
• Status: Expired due to Term

• Current Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Original Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Inventors: Karabutov, Alexander A., Oraevsky, Alexander A.
• Primary Examiner(s): Lateef, Marvin M.
• Assistant Examiner(s): SHAW, SHAWNA JEANNINE
• Application Number:
  US09/412,852
• Time in Patent Office: 979 Days
• Field of Search: 600/407, 600/437, 600/316, 600/310, 600/586, 600/322, 600/438, 600/478, 600/319, 606/3, 606/10, 606/11, 606/13, 735/87, 736/06, 367/7
• US Class Current: 600/407
• CPC Class Codes: A61B 2017/00128 related to intensity or progress of surgical action
• CPC Class Codes: A61B 5/0095 by applying light and detecting acoustic waves, i.e. photoacoustic measurements
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/6821 Eye
• CPC Class Codes: A61B 8/08 Detecting organic movements or changes, e.g. tumours, cysts, swellings A61B8/02 - A61B8/06 take precedence
• CPC Class Codes: A61B 8/0825 for diagnosis of the breast, e.g. mammography
• CPC Class Codes: A61B 90/36 Image-producing devices or illumination devices not otherwise provided for
• CPC Class Codes: A61F 2009/00844 Feedback systems
• CPC Class Codes: A61F 2009/00865 Sclera
• CPC Class Codes: A61F 2009/00897 Scanning mechanisms or algorithms
• CPC Class Codes: A61F 9/008 using laser
• CPC Class Codes: A61K 49/225 Microparticles, microcapsules gas-filled to be classified in A61K49/223
• CPC Class Codes: G01N 21/1702 with opto-acoustic detection, e.g. for gases or analysing solids

Abstract

The present invention is directed to a method/system of monitoring in real time changes in concentration of glucose in tissues. Laser-induced profiles of absorbed optical energy distribution in tissues are determined via measurements of spatial (in-depth) profile of optically-induced acoustic (pressure) transients using a wide-band optoacoustic transducer. Such technique can be applied for monitoring of glucose concentration in various human or nonhuman tissues, cell cultures, solutions or emulsions.

Claims

1. A method of noninvasive monitoring of glucose concentration in real time using laser optoacoustic imaging, comprising the steps of:

irradiating a tissue of interest with at least one optical pulse to create a distribution of absorbed optical energy in said tissue;

generating an optothermally-induced pressure profile under conditions of temporal pressure confinement in the irradiated tissue;

detecting said pressure profile with at least one wide-band acoustic transducer, wherein said acoustic transducer is capable of detecting the entire range of ultrasonic frequencies contained in said pressure profile;

recording an amplitude and temporal profile of said pressure profile by an electronic system;

analyzing the ultrasonic frequency of said pressure profile, wherein a change in the ultrasonic frequency indicates a change in tissue scattering coefficient, which in turn is proportional to a change in glucose concentration in said tissue, which correlates with a change of glucose concentration in blood.

2. The method of claim 1, wherein said tissue is selected from the group consisting of sclera, ear lobe and finger.

3. The method of claim 1, wherein said optical pulse is generated from a laser or other pulsed optical source.

4. The method of claim 1, wherein said optical pulse is in the spectral range from about 0.2 μ

m to about 20 μ

m.

5. The method of claim 1, wherein said optical pulse is in the range of nanoseconds.

6. The method of claim 1, wherein said acoustic transducer is a wide-band acoustic transducer.

7. The method of claim 1, wherein said pressure profile detection is in forward signal propagation mode as said irradiation and said acoustic detection are performed at different sites in said tissue.

8. The method of claim 1, wherein said pressure profile detection is in backward signal propagation mode as said irradiation and said acoustic detection are performed at a same site in said tissue.

9. The method of claim 1, wherein said irradiating is in a spectral range of from about 300 nm to about 2400 nm.

10. The method of claim 1, further comprising the step of:

administering an exogenous substance to said tissue, wherein said substance reacts selectively with glucose and enhances the effect of glucose on tissue scattering.

11. The method of claim 10, wherein said exogenous substance is a molecules that change optical refractive index of tissue.

12. The method of claim 1, wherein said detecting step is performed by scanning of a single transducer along said tissue.

13. The method of claim 1, wherein said detecting step is performed by scanning of an array of transducers along said tissue.

14. The method of claim 1, wherein said pressure profile is recorded simultaneously from a number of sites along a surface of said tissue in order to reconstruct a two-dimensional or three-dimensional topographic image of glucose distribution in tissue.

15. The method of claim 1, wherein said irradiating is carried out with at least one optical pulse from each of two or more optical sources and/or wherein said irradiating is carried out with at least two optical pulses having at least two different wavelengths.

16. A system comprising:

a pulsed optical source to produce a pressure profile confined in a volume of tissue of interest;

a light delivery system for delivery of radiation to said tissue;

at least one wide-band acoustic transducer operating in the frequency range of from 900 kHz to 200 MHz to detect the pressure profile in said tissue, wherein said acoustic transducer is capable of detecting the entire range of ultrasonic frequencies contained in the pressure profile; and

an electronic system for recording and processing of said detected pressure profile.

17. The system of claim 16, wherein said pulsed optical source generates optical pulses as short as measured in nanoseconds.

18. The system of claim 16, wherein said system is used to determine the distribution of glucose in the monitored tissue.

19. A system of claim 16, wherein said optoacoustic profile is used to monitor changing optical properties in human tissue, in order to determine optimal parameters for therapeutic or surgical treatment.