Process for the synthesis of modified P-chiral nucleotide analogues
First Claim
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1. A process for the synthesis of modified P-chiral nucleotide analogues of Formula 1:
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wherein;
R1 is a protecting group;
R2 is selected from hydrogen, protected hydroxyl group, vinyl, halogen, nitrile, azide, protected amine group, chloroalkyl, perfluoroalkyl, perfluoroalkoxyl, alkoxyalkyl, ethynyl, OQ1, SQ1, NHQ1, where Q1 stands for alkyl, aryl, alkenyl or alkynyl;
B is a purine or pyrimidine base;
Z is selected from alkyl, aryl, alkenyl, alkynyl, vinyl, ethynyl, aminomethyl or aminoethyl substituents;
X is an oxygen, sulfur or selenium atom;
Rx is a protecting group;
said process comprising the steps of;
(a) providing the following compound of Formula 2;
whereinR1, R2 and B are as defined above;
Y is an XR3 substituent, wherein X is an oxygen, sulfur or selenium atom and R3 is an acyl group of formula COR4, in which R4 is an alkyl group, perfluoroalkyl group, or an aryl substituent containing six to fifteen carbon atoms and having an aromatic ring comprising mono-, di- or tri-substituted aromatic substituents activating the aromatic ring;
(b) providing the following compound of Formula 6;
wherein;
B, R2 and Rx are as defined above;
(c) reacting the compound of Formula 6 with the compound of Formula 2 under anhydrous conditions, in an aprotic organic solvent, in the presence of at least one activating reagent, to yield the compound of Formula 1; and
(d) isolating the compound of Formula 1.
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Abstract
The process of the instant invention is drawn to the synthesis of modified P-chiral nucleotide analogues in the form of pure diastereomers possessing preselected configuration at the P-atom. Oligonucleotides prepared by the method of the invention containing P-chiral compounds have enhanced hybridization and transporting properties.
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Citations
23 Claims
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1. A process for the synthesis of modified P-chiral nucleotide analogues of Formula 1:
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wherein; R1 is a protecting group;
R2 is selected from hydrogen, protected hydroxyl group, vinyl, halogen, nitrile, azide, protected amine group, chloroalkyl, perfluoroalkyl, perfluoroalkoxyl, alkoxyalkyl, ethynyl, OQ1, SQ1, NHQ1, where Q1 stands for alkyl, aryl, alkenyl or alkynyl;
B is a purine or pyrimidine base;
Z is selected from alkyl, aryl, alkenyl, alkynyl, vinyl, ethynyl, aminomethyl or aminoethyl substituents;
X is an oxygen, sulfur or selenium atom;
Rx is a protecting group;
said process comprising the steps of;
(a) providing the following compound of Formula 2;
wherein R1, R2 and B are as defined above;
Y is an XR3 substituent, wherein X is an oxygen, sulfur or selenium atom and R3 is an acyl group of formula COR4, in which R4 is an alkyl group, perfluoroalkyl group, or an aryl substituent containing six to fifteen carbon atoms and having an aromatic ring comprising mono-, di- or tri-substituted aromatic substituents activating the aromatic ring;
(b) providing the following compound of Formula 6;
wherein; B, R2 and Rx are as defined above;
(c) reacting the compound of Formula 6 with the compound of Formula 2 under anhydrous conditions, in an aprotic organic solvent, in the presence of at least one activating reagent, to yield the compound of Formula 1; and
(d) isolating the compound of Formula 1. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
(i) phosphorylating with a phosphorylating reagent, a substrate of Formula 3;
wherein R1, R2 and B are as defined previously, said phosphorylating reagent comprising a compound of Formula 4;
wherein; Z is as defined previously, and W is a halogen; and
(ii) followed by hydrolysis without isolation of the intermediate of Formula 5;
where R1, R2, B, Z and X are as defined in claim 1, and Y is an oxygen atom, to yield the compound of Formula 2.
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6. The process according to claim 1, wherein the aprotic organic solvent comprises tetrahydrofuran or acetonitrile.
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7. The process according to claim 1, wherein the at least one activating reagent is an organic base amine.
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8. The process according to claim 1, wherein the reacting in step (c) comprises an additional activating reagent which is a lithium salt.
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9. The process according to claim 1, wherein the compound of Formula 2 is a diastereoisomer which possesses an absolute configuration at the P-atom identical with that desired the analogues of Formula 1, said diastereoisomer being reacted with the compound of Formula 6 under anhydrous conditions, in the aprotic organic solvent, in the presence of the at least one activating reagent, to yield the compound of Formula 1.
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10. The process according to claim 9, wherein the compound of Formula 2 is obtained by phosphorylation of a substrate of Formula 3:
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with a phosphorylating reagent of Formula 4;
followed by hydrolysis without isolation of an intermediate of Formula 5;
to yield a compound of Formula 2 wherein R1, R2, B, Z and X are as defined previously, and Y is an oxygen atom.
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11. The process according to claim 9, wherein the aprotic organic solvent is tetrahydrofaran or acetonitrile.
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12. The process according to claim 9, wherein the at least one activating reagent is an organic base.
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13. The process according to claim 9, wherein the reacting in step (c) is in the presence of an additional activating reagent which is a lithium salt.
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14. A process for the synthesis of modified P-chiral nucleotide analogues of Formula 1 in the form of a pure diastereoisomer possessing a preselected configuration at the P-atom:
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wherein; R1 is a protecting group;
R2 is selected from hydrogen, protected hydroxyl group, vinyl, halogen, nitrile, azide, protected amine group, chloroalkyl, perfluoroalkyl, perfluoroalkoxyl, alkoxyalkyl, vinyl, ethynyl, OQ1, SQ1, NHQ1, where Q1 stands for alkyl, aryl, alkenyl or alkynyl;
B is a purine or pyrimidine base;
Z is selected from alkyl, aryl, alkenyl, alkynyl, vinyl, ethynyl, aminomethyl or aminoethyl substituents;
Rx is a protecting group;
Y is an XR3 substituent, where X is an oxygen, sulfur or selenium atom and R3 is an acyl group of formula COR4, in which R4 is an alkyl group, perfluoroalkyl group, or an aryl substituent containing six to fifteen carbon atoms and having an aromatic ring comprising mono-, di- or tri-substituted aromatic substituents activating the aromatic ring;
said process comprising the steps of;
(a) hydrolyzing a diastereoisomer of the compound of Formula 2;
where; R1, R2 and B are as defined above;
Y is an oxygen;
X is a sulfur or selenium atom, wherein the compound of Formula 2 possesses an absolute configuration at the P-atom opposite to that desired for the analogues of Formula 1, said hydrolyzing being done in the presence of an activator that inverts the absolute configuration of the P-atom resulting in a product of Formula 2, (b) reacting the product of step (a) with a compound of Formula 7;
wherein R4 is an alkyl, perfluoroalkyl, or a aroyl substituent containing six to fifteen carbon atoms, and having an aromatic ring comprising mono-, di- or tri-substituted aromatic substituents activating the aromatic ring; and
W is a chlorine, bromine or iodine atom;
to yield the compound of Formula 2, where R1, R2, B and Z are as defined above, X is a sulfur or selenium atom, and Y is R4C(O)O—
, in which R4 is as defined above, wherein the compound of Formula 7 possesses an absolute configuration at the P-atom opposite to that of the diastereoisomer of Formula 2, and identical to that which is desired for the analogues of Formula 1.- View Dependent Claims (15, 17, 18, 19, 20, 23)
(i) phosphorylating with a phosphorylating reagent, a substrate of Formula 3;
wherein R1, R2 and B are as defined previously;
said phosphorylating reagent comprising a compound of Formula 4;
wherein Z and X are as defined previously, W is a halogen;
(ii) followed by hydrolysis without isolation of the intermediate of Formula 5;
where R1, R2, B, Z and X are defined previously, and Y is an oxygen atom to yield the compound of Formula 2.
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18. The process according to claim 17, further comprising:
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(i) separating chromatographically the compound of Formula 2 into two diastereoisomers; and
(ii) reacting the diasterosiomer with compound of Formula 7;
wherein; R4 is an alkyl group, perfluoroalkyl group, or an aroyl substituent containing six to fifteen carbon atoms and having an aromatic ring comprising mono-, di- or tri-substituted aromatic substituents activating the aromatic ring, and W is a halogen.
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19. The process according to claim 14, wherein the activator is an organic base amine.
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20. The process according to claim 14, wherein the aprotic organic solvent is tetrahydrofuran.
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23. The process according to claim 14, wherein X is an oxygen, sulfur or selenium atom, and Y of Formula 2 is R4C(O)O—
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16. A process for the synthesis of modified P-chiral nucleotide analogues of Formula 1 in the form of a pure diastereoisomer possessing a preselected configuration at the P-atom:
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wherein; R1 is a protecting group;
R2 is selected from hydrogen, protected hydroxyl group, vinyl, halogen, nitrile, azide, protected amine group, chloroalkyl, perfluoroalkyl, perfluoroalkoxyl, alkoxyalkyl, vinyl, ethynyl, OQ1, SQ1, NHQ1, where Q1 stands for alkyl, aryl, alkenyl or alkynyl;
B is a purine or pyrimidine base;
Z is selected from alkyl, aryl, alkenyl, alkynyl, vinyl, ethynyl, aminomethyl or aminoethyl substituents;
Rx is a protecting group;
Y is an XR3 substituent, where X is an oxygen, sulfur or selenium atom and R3 is an acyl group of formula COR4, in which R4 is an alkyl group, perfluoroalkyl group, or an aryl substituent containing six to fifteen carbon atoms and having an aromatic ring comprising mono-, di- or tri-substituted aromatic substituents activating the aromatic ring;
said process comprising the steps of;
(a) hydrolyzing a diastereoisomer of the compound of Formula 2;
wherein;R1, R2 and B are as defined above;
Y is an oxygen;
X is a sulfur or selenium atom, wherein the compound of Formula 2 possesses an absolute configuration at the P-atom opposite to that desired for the analogues of Formula 1, said hydrolyzing being done in the presence of an activator that inverts the absolute configuration of the P-atom resulting in a product of Formula 2, (b) reacting the product of step (a) with a compound of Formula 7;
whereinR4 is an alkyl, perfluoroalkyl, or a aroyl substituent containing six to fifteen carbon atoms, and having an aromatic ring comprising mono-, di- or tri-substituted aromatic substituents activating the aromatic ring; and
W is a chlorine;
bromine or iodine atom, to yield the compound of Formula 2, where R1, R2, B and Z are as defined above, X is a sulfur or selenium atom, andY is R4C(O)O—
, in which R4 is as defined above, wherein the compound of Formula 7 possesses an absolute configuration at the P-atom opposite to that of the diastereoisomer of Formula 2, and identical to that which is desired for the analogues of Formula 1; and
(c) reacting the product with compound of Formula 6;
wherein B, R2 and Rx are as defined previously, under anhydrous conditions, in an aprotic organic solvent, in the presence of an activating reagent, to yield the compound of Formula 1 and isolating the compound of Formula 1. - View Dependent Claims (21)
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22. A process for the synthesis of modified P-chiral nucleotide analogues of Formula 1 in the form of a pure diastereoisomer possessing a preselected configuration at the P-atom:
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wherein; R1 is a protecting group;
R2 is selected from hydrogen, protected hydroxyl group, vinyl, halogen, nitrile, azide, protected amine group, chloroalkyl, perfluoroalkyl, perfluoroalkoxyl, alkoxyalkyl, vinyl, ethynyl, OQ1, SQ1, NHQ1, where Q1 stands for alkyl, aryl, alkenyl or alkynyl;
Y is an oxygen;
X is a sulfur or selenium atom wherein the compound of Formula 2 possesses an absolute configuration at the P-atom opposite to that desired for the analogues of Formula 1, comprising the steps of;
(a) hydrolizing one of two diastereoisomers of formula 2 in the presence of an activator that inverts the absolute configuration of the P-atom resulting in a product of Formula 2 (b) reacting the product of step (a) with a compound of Formula 7;
wherein R4 is an alkyl, perfluoroalkyl, or a aroyl substituent containing six to fifteen carbon atoms, and having an aromatic ring comprising mono-, di- or tri-substituted aromatic substituents activating the aromatic ring; and
W is a chlorine, bromine or iodine atom, to yield the compound of Formula 2, where R1, R2, B and Z are as defined above, X is a sulfur or selenium atom, and Y is R4C(O)O—
, in which R4 is as defined above, wherein the compound of Formula 7 possesses an absolute configuration at the P-atom opposite to that of the diastereoisomer of Formula 2, and identical to that which is desired for the analogues of Formula 1; and
(c) providing the following compound of Formula 6;
wherein B, R2 and Rx are as defined previously;
(d) reacting the compound of Formula 6 with the compound of Formula 2 under anhydrous conditions, in an aprotic organic solvent, in the presence of an activating reagent, to yield compound of Formula 1 of desired absolute configuration at the P-atom; and
(e) isolating the compound of Formula 1.
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Specification